Abstract:

Objective To investigate the role of reticulocyte（RET） parameters in the treatment of hemolytic disease of newborns（HDN）,and to provide a reference for evaluating treatment efficiency. Methods Peripheral blood samples of 44 patients with HDN were collected on the days of admission,treatment and discharging,respectively. All samples were determined for reticulocyte percentage（RET%）,immature reticulocyte fraction percentage （IRF%）,low fluorescence reticulocyte percentage （LFR%）,medium fluorescence reticulocyte percentage （MFR%） and high fluorescence reticulocyte percentage （HFR%）. The results on the day of admission were as baselines,and the change trend of the results on the days of treatment and discharging were evaluated. Results The RET%,IRF%,MFR% and HFR% decreased,and LFR% showed an increase with the treatment progressed. RET% was decreased,which accounted for 84.09%. In decreased group,RET%,IRF%,MFR% and HFR% on the day of discharging had statistical significance compared with those on the day of admission（P<0.05）. The decrease of RET% was positively correlated with those of IRF% and HFR% in decreased group（r=0.632 and 0.639,P<0.01）. There was no statistical significance for RET%,IRF%,MFR% and HFR% between the days of admission and discharging （P>0.05）. Conclusions RET% could be used as a sensitive indicator in monitoring the treatment of HDN. The decreasing of RET% indicates that the treatment is effective. IRF%,HFR% and LFR% could be used as auxiliary indicators for monitoring HDN treatment.

Key words: Reticulocyte, Immature reticulocyte, Hemolytic disease of newborn