Influence of reanalysis time for polymorphonuclear neutrophil elastase on male genital tract infection patients

doi:10.3969/j.issn.1673-8640.2018.05.005

Abstract

Abstract:

Objective To investigate the influence of polymorphonuclear neutrophil elastase （PMNE） on the quality of semen,and to investigate optimal reanalysis time for PMNE. Methods The quality of semen of 763 patients were determined by SCA-H-01 computer-assisted sperm analysis,and PMNE level was determined by enzyme-linked immunosorbent assay （ELISA）. According to PMNE level,genital tract infection was identified. After treatment for inapparent and confirmed infection patients, different reanalysis times were chosen and evaluated. Results Sperm motility of PMNE confirmed infection patients was decreased（P<0.05）. The positive rate of PMNE in patients reanalyzed in <7 d had statistical significance compared with those in 7-14 d （χ²=7.601,P=0.006）and >14 d （χ²=18.530,P<0.001）. Conclusions The increasing of PMNE level may influence on semen quality,and it is important for choosing a proper time to reanalyze PMNE level and obtain exact results.

Key words: Polymorphonuclear neutrophil elastase, Genital tract infection, Reanalysis time

CLC Number:

R446.1

CHENG Zhaojun, ZHOU Wenjing, FENG Ying, WANG Chong. Influence of reanalysis time for polymorphonuclear neutrophil elastase on male genital tract infection patients[J]. Laboratory Medicine, 2018, 33(5): 396-398.

Figures/Tables 2

References 18

[1]	WEIDNER W,COLPI G M,HARGREAVE T B,et al.EAU guidelines on male infertility[J]. Eur Urol,2002,42(4):313-322.
[2]	SAKKAS D,ALVAREZ J G.Sperm DNA fragmentation:mechanisms of origin,impact on reproductive outcome,and analysis[J]. Fertil Steril,2010,93(4):1027-1036.
[3]	EISENBERG M L,LIPSHULTZ L I.Varicocele-induced infertility:newer insights into its pathophysiology[J]. Indian J Urol,2011,27(1):58-64.
[4]	王晟,贺占举,郐艳荣,等. 精液中白细胞与精液质量的关系研究[J]. 中国性科学,2013,22(5):3-6.
[5]	魏小斌,白志明,李春芸,等. 不育症患者精浆弹性蛋白酶检测的临床意义[J]. 中国实验诊断学,2009,13(11):1563-1566.
[6]	ZORN B,SESEK-BRISKI A,OSREDKAR J,et al.Semen polymorphonuclear neutrophil leukocyte elastase as a diagnostic and prognostic marker of genital tract inflammation-a review[J]. Clin Chem Lab Med,2003,41(1):2-12.
[7]	KOPA Z,WENZEL J,PAPP G K,et al.Role of granulocyte elastase and interleukin-6 in the diagnosis of male genital tract inflammation[J]. Andrologia,2005,37(5): 188-194.
[8]	聂尚丹,陈廷,李雷生,等. 精浆弹性蛋白酶水平、白细胞定量与男性不育的关系[J]. 中国医药导报,2010,7(14):16-18.
[9]	石晓星,商学军. 中性粒细胞弹性蛋白酶在男性生殖道感染诊断中的意义[J]. 中华男科学,2003,9(2):136-139.
[10]	秦松林. 男性不育患者精浆中弹性蛋白酶水平和白细胞定量及其与精液质量的关系研究[J]. 航空航天医学杂志,2014,25(1):19-21.
[11]	张涛,陈斌,吕逸清,等. 抗炎治疗对白细胞精液症患者精浆瘦素与活性氧的影响分析[J]. 中国男科学杂志,2014,29(3):52-55.
[12]	林楚怀,周辉谱. 男性泌尿生殖道性病感染的检验与疗效分析[J]. 检验医学与临床,2014,11(2):171-172.
[13]	江传宁. 泌尿外科感染的原因与治疗探讨[J]. 医学信息,2015,28(37):246-247.
[14]	EGGERT-KRUSE W,ZIMMERMANN K,GEISSLER W,et al.Clinical relevance of polymorphonuclear (PMN-) elastase determination in semen and serum during infertility investigation[J]. Int J Androl,2009,32(32):317-329.
[15]	HENKEL R,MAASS G,JUNG A,et al.Age-related changes in seminal polymorphonuclear elastase in men with asymptomatic inflammation of the genital tract[J]. Asian J Androl,2007,9(3):299-304.
[16]	ALLAM J P,FRONHOFFS F,FATHY A,et al.High percentage of apoptotic spermatozoa in ejaculates from men with chronic genital tract inflammation[J]. Andrologia,2008,40(5):329-334.
[17]	毛金观,彭忠英,杨戎威,等. 生殖道感染不育患者精浆生化指标与精液参数的相关分析[J]. 浙江医学,2016,38(18):1519-1520.
[18]	冯瑞祥,卢坤刚,张红烨,等. 精液中性粒细胞弹性蛋白酶的研究进展[J]. 中华男科学杂志,2011,17(11):1023-1028.

组别	例数	精子浓度（×10⁶/mL）	前向活动精子（%）
未感染组	208	71.45±7.39	53.92±11.26
隐性感染组	319	66.17±6.55	51.58±5.69
确证感染组	236	63.27±9.13	43.27±7.43^*

组别	例数	精子浓度（×10⁶/mL）	前向活动精子（%）
未感染组	208	71.45±7.39	53.92±11.26
隐性感染组	319	66.17±6.55	51.58±5.69
确证感染组	236	63.27±9.13	43.27±7.43^*

分组	例数	复查例数	PMNE阳性[例（%）]
隐性感染组	312
早期复查组		231	53（22.9）
中期复查组		61	9（14.8）
晚期复查组		20	2（10.0）
确证感染组	213
早期复查组		162	103（63.6）
中期复查组		30	11（36.7）^*
晚期复查组		21	3（14.3）^*

分组	例数	复查例数	PMNE阳性[例（%）]
隐性感染组	312
早期复查组		231	53（22.9）
中期复查组		61	9（14.8）
晚期复查组		20	2（10.0）
确证感染组	213
早期复查组		162	103（63.6）
中期复查组		30	11（36.7）^*
晚期复查组		21	3（14.3）^*