HIV-1新型重组毒株近似全长基因组结构鉴定分析

doi:10.3969/j.issn.1673-8640.2023.11.002

检验医学 ›› 2023, Vol. 38 ›› Issue (11): 1015-1019.DOI: 10.3969/j.issn.1673-8640.2023.11.002

HIV-1新型重组毒株近似全长基因组结构鉴定分析

路新利¹, 刘勇², 李岩¹, 王莹莹¹, 安宁¹, 刘萌¹

1.河北省疾病预防控制中心艾滋病防治所，河北石家庄 050021
2.河北省邱县中心医院，河北邯郸 057450

收稿日期:2022-05-12 修回日期:2023-01-09 出版日期:2023-11-30 发布日期:2024-01-10
作者简介:路新利，男，1975年生，博士，副主任技师，主要从事人类免疫缺陷病毒分子流行病学研究。
基金资助:
河北省医学科学研究重点课题计划(20160061);河北省自然科学基金项目(H2016303006)

Identification of near full-length genome structures of new HIV-1 recombinant strains

LU Xinli¹, LIU Yong², LI Yan¹, WANG Yingying¹, AN Ning¹, LIU Meng¹

1. AIDS Prevention and Control Institute，Hebei Provincial Center for Disease Control and Prevention，Shijiazhuang 050021，Hebei，China
2. The Central Hospital of Qiuxian，Handan 057450，Hebei，China

Received:2022-05-12 Revised:2023-01-09 Online:2023-11-30 Published:2024-01-10

摘要/Abstract

摘要：

目的对3条人类免疫缺陷病毒（HIV）-1亚型不确定的近似全长基因组序列（NFLG）进行基因镶嵌结构分析。方法用近末端稀释法、系统发育树和基因重组断点分析等方法对3例男男同性恋（MSM）HIV-1感染病例进行HIV-1 NFLG扩增、基因亚型分析和基因重组图谱分析。结果 Neighbor-joining进化树分析结果显示，3条HIV-1序列均为单独分支，可能是新型重组毒株。jpHMM在线软件和SimPlot v3.5.1软件综合分析结果显示，NFLG 110和NFLG 171分别为CRF07_BC和CRF01_AE，NFLG 150为CRF01_AE和CRF07_BC二代重组毒株。基因重组断点分析结果显示，NFLG 150的重组模式是以CRF07_BC全长基因组为骨架，在gag区和env区分别插入了1个长度约为60和80 bp的CRF01_AE基因片段。结论在MSM人群中发现1株HIV-1 CRF01_AE/CRF07_BC二代重组毒株，建议持续监测相关性传播，特别是MSM人群HIV-1新型基因重组毒株。

关键词: 人类免疫缺陷病毒, 二代重组基因, 系统发育树, 基因重组断点分析, 近似全长基因组序列

Abstract:

Objective To analyze the near full-length genome（NFLG） structures of 3 human immunodeficiency virus（HIV）-1 with unknown subtypes. Methods The NFLG of 3 HIV-1 men who have sex with men（MSM） were amplified by near-terminal dilution method，and subtypes and genetic recombinant maps were confirmed by the analysis of phylogenetic tree and gene recombination breakpoints. Results The results of Neighbor-joining evolutionary tree analysis showed that all the 3 HIV-1 sequences were separate branches，which might be new recombinant strains. The comprehensive analysis results of online jpHMM and SimPlot v3.5.1 software showed that NFLG 110 and NFLG 171 were CRF07_BC and CRF01_AE，respectively，and NFLG 150 were CRF01_AE and CRF07_BC second-generation recombinant strains. The results of gene recombination breakpoint analysis showed that the recombination mode of NFLG 150 was based on the full-length CRF07_BC genome as skeleton，and CRF01_AE gene fragments with lengths of about 60 and 80 bp were inserted into gag and env regions，respectively. Conclusions New HIV-1 recombinant strains from CRF01_AE and CRF07_BC have been found in MSM population. It is critical for us to monitor continually new HIV-1 recombinant strains in the sexual contact population especially MSM.

Key words: Human immunodeficiency virus, Second-generation recombinant gene, Phylogenetic tree, Gene recombination breakpoint, Near full-length genome sequence

中图分类号:

R373.9

路新利, 刘勇, 李岩, 王莹莹, 安宁, 刘萌. HIV-1新型重组毒株近似全长基因组结构鉴定分析[J]. 检验医学, 2023, 38(11): 1015-1019.

LU Xinli, LIU Yong, LI Yan, WANG Yingying, AN Ning, LIU Meng. Identification of near full-length genome structures of new HIV-1 recombinant strains[J]. Laboratory Medicine, 2023, 38(11): 1015-1019.

图/表 4

图1 基于NFLG的neighbor-joining进化树注：●代表研究序列；Bootstrap值≥70%被显示。

图2 近似全长基因组的jpHMM重组分析

图3 病例150的NFLG BootScan分析图谱

图4 病例110和171的NFLG BootScan分析图谱注：（a）病例110；（b）病例171。

参考文献 20

[1]	MICHAEL S. Pneumocystis Pneumonia—Los Angeles[J]. Am J Public Health, 2006, 96(6):980-981. DOI URL
[2]	UNAIDS. 2021 Progress Report on the Global AIDS response. Available from: https://www.sohu.com/a/477822682_121106902.
[3]	GASCHEN B, TAYLOR J, YUSIM K, et al. Diversity considerations in HIV-1 vaccine selection[J]. Science, 2002, 296(5577):2354-2360. PMID
[4]	张菲, 梁华悦, 钟姗妹, 等. 广西一株HIV-1 CRF01_AE/CRF07_BC独特重组毒株的鉴定[J]. 中国艾滋病性病, 2021, 27(6):563-569.
[5]	LI X, LI W, ZHONG P, et al. Nationwide trends in molecular epidemiology of HIV-1 in China[J]. AIDS Res Hum Retroviruses, 2016, 32(9):851-859. DOI URL
[6]	LI Z, LIAO L, FENG Y, et al. Trends of HIV subtypes and phylogenetic dynamics among young men who have sex with men in China,2009-2014[J]. Sci Rep, 2015, 5:16708. DOI
[7]	CHEN J, YOUNG N, SUBBARAO S, et al. HIV type 1 subtypes in Guangxi Province,China,1996[J]. AIDS Res Hum Retroviruses, 1999, 15(1):81-84. DOI URL
[8]	CHENG H, ZHANG J, CAPIZZI J, et al. HIV-1 subtype E in Yunnan,China[J]. Lancet, 1994, 344(8927):953-954.
[9]	FENG Y, YUTAKA T, WEI H, et al. Geographic origin and evolutionary history of China's two predominant HIV-1 circulating recombinant forms,CRF07_BC and CRF08_BC[J]. Sci Rep, 2014, 6:19279. DOI
[10]	LU X, ZHAO C, WANG W, et al. HIV-1 genetic diversity and its distribution characteristics among newly diagnosed HIV-1 individuals in Hebei Province,China[J]. AIDS Res Ther, 2016, 13:3. DOI URL
[11]	LU X, KANG X, LIU Y, et al. HIV-1 molecular epidemiology among newly diagnosed HIV-1 individuals in Hebei,a low HIV prevalence province in China[J]. PLoS One, 2017, 12(2):e0171481. DOI URL
[12]	FENG Y, HE X, HSI J H, et al. The rapidly expanding CRF01_AE epidemic in China is driven by multiple lineages of HIV-1 viruses introduced in the 1990s[J]. AIDS, 2013, 27(11):1793-1802. DOI PMID
[13]	LU X, ZHANG J, WANG Y, et al. Large transmission clusters of HIV-1 main genotypes among HIV-1 individuals before antiretroviral therapy in the Hebei Province,China[J]. AIDS Res Hum Retroviruses, 2020, 36(5):427-433. DOI URL
[14]	LIANG B, YANG Y, ZHANG F, et al. Characterization of a novel HIV-1 recombinant form(CRF01_AE/CRF07_BC/CRF08_BC) identified from Guangxi,China[J]. AIDS Res Hum Retroviruses, 2020, 36(2):143-152. DOI URL
[15]	WU L, HUANG L, ZHANG W, et al. Characterization of a novel HIV-1 CRF01_AE/CRF07_BC recombinant virus form in Guizhou,China[J]. AIDS Res Hum Retroviruses, 2019, 35(7):664-667. DOI URL
[16]	HE S, GAO Y, AN M, et al. Characterization of a novel HIV-1 CRF01_AE/CRF07_BC recombinant strain among men who have sex with men in Liaoning,China[J]. AIDS Res Hum Retroviruses, 2021, 37(1):70-74. DOI URL
[17]	ZHOU J, LU X, FENG Y, et al. Genome sequence of a novel HIV-1 circulating recombinant form(CRF103_01B) identified from Hebei Province,China[J]. AIDS Res Hum Retroviruses, 2020, 36(3):234-241. DOI URL
[18]	HAN L, LI H, WANG L, et al. Near full-length genomic characterization of two novel HIV-1 unique recombinant forms(CRF01_AE/CRF07_BC)among MSM in Shijiazhuang city,Hebei province,China[J]. AIDS Res Hum Retroviruses, 2021, 37(12):978-984. DOI URL
[19]	XING Y, GUO Y, WANG L, et al. Identification of two novel HIV-1 second-generation recombinant forms(CRF01_AE/CRF07_BC) in Hebei,China[J]. AIDS Res Hum Retroviruses, 2021, 37(12):967-972. DOI URL
[20]	王伟, 马琳, 吴小雪, 等. 2010—2019年河北省艾滋病流行特征分析[J]. 中国病原生物学杂志, 2021, 16(7):800-804.

HIV-1新型重组毒株近似全长基因组结构鉴定分析

Identification of near full-length genome structures of new HIV-1 recombinant strains

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 20

相关文章 15

编辑推荐

Metrics

[1]	赵静, 李永勤, 李艳, 范伟光, 杨学刚. HIV/AIDS合并机会性感染患者IP-10、SAA、hs-CRP、PCT、CD4⁺ T细胞计数检测的意义[J]. 检验医学, 2023, 38(8): 725-729.
[2]	代芳芳, 鲁辛辛, 于艳华, 陈铭, 孙桂珍. HIV/AIDS患者分枝杆菌检出情况分析[J]. 检验医学, 2023, 38(12): 1173-1176.
[3]	邓卓娜, 杨令芝, 黄茂, 王烤, 宁旭. 疑似疱疹病毒抗体引起HIV初筛假阳性1例报道[J]. 检验医学, 2023, 38(10): 1006-1008.
[4]	孙琦, 曲新艺, 徐莉. 免疫印迹法HIV-1抗体检测结果不确定者的转归分析[J]. 检验医学, 2022, 37(8): 741-744.
[5]	范伟光, 苏苗苗, 孟娟, 石鹏辉, 张珍. AIDS患者抗病毒治疗中IP-10水平的变化及临床意义[J]. 检验医学, 2022, 37(3): 221-225.
[6]	黄山, 孙溪若, 吕松琴, 许宝妹, 聂磊, 王义娜, 李晓非. 干式荧光发光法在HIV感染初筛中的应用价值[J]. 检验医学, 2022, 37(2): 174-176.
[7]	王少华, 苍金荣, 王翠, 崔晓利, 康欢欢. 疑似结核性腹膜炎获得性免疫缺陷综合征患者合并马尔尼菲蓝状菌病1例报道[J]. 检验医学, 2021, 36(7): 773-775.
[8]	王万海, 孟真, 杨若男, 高楠, 王庆芳, 张磊, 王梅芬, 明亮. 免疫印迹法检测HIV抗体条带常见模式分析及在随访中的价值[J]. 检验医学, 2020, 35(2): 95-99.
[9]	杨勇, 蒲伟, 罗小虎. 国产全自动HIV-1核酸定量检测试剂盒的性能评价[J]. 检验医学, 2020, 35(2): 153-155.
[10]	刘懿萱, 郭林, 卢仁泉. 获得性免疫缺陷综合征相关淋巴瘤诊疗的研究进展[J]. 检验医学, 2020, 35(2): 173-177.
[11]	孙斌, 王姗, 钱俊, 唐振华. 63 109例上海地区孕妇部分产前筛查指标分析[J]. 检验医学, 2017, 32(5): 386-389.
[12]	韦善求. HIV感染疾病进程相关免疫因素研究进展[J]. 检验医学, 2015, 30(6): 646-649.
[13]	张春雷, 李忠新, 张书楠, 陈桂冰, 曾学辉. 复合质控血清研制及临床应用评价[J]. 检验医学, 2015, 30(3): 292-294.
[14]	郭川, 纪琳莹, 吴耀波, 林洁敏, 陈婉. 不同临床分期HIV-1感染者/AIDS患者血样的蛋白印迹试验带型分析[J]. 检验医学, 2015, 30(2): 145-148.
[15]	肖敏敏，王毅，邵慧，张艳，李萍. 皖南地区HIV感染者中HCV感染状况的调查[J]. 检验医学, 2014, 29(7): 705-707.