肠道菌群对慢性荨麻疹患者血清炎症因子的影响

doi:10.3969/j.issn.1673-8640.2021.05.009

摘要/Abstract

摘要：

目的初步探讨肠道菌群对慢性荨麻疹患者血清炎症因子的影响。方法收集60例慢性荨麻疹患者和60名体检健康者空腹静脉血、粪便样本。采用16S rDNA基因实时荧光定量聚合酶链反应（PCR）检测所有研究对象肠道普拉梭菌、柔嫩梭菌、双歧杆菌和乳酸杆菌水平;采用气相色谱检测肠道短链脂肪酸;采用酶联免疫吸附试验（ELISA）检测血清Toll样受体（TLR）4、核因子（NF）-κB、肿瘤坏死因子α（TNF-α）、白细胞介素-6（IL-6）水平。采用Spearman相关性分析分析肠道细菌、短链脂肪酸与血清TLR4、NF-κB、TNF-α和IL-6的关系。结果与体检健康者比较,慢性荨麻疹患者肠道普拉梭菌、柔嫩梭菌、乳酸杆菌丰度及丁酸水平明显下降（P<0.05）;血清TLR4、NF-κB、TNF-α和IL-6水平明显上升（P<0.05）。慢性荨麻疹患者肠道普拉梭菌、柔嫩梭菌、乳酸杆菌、丁酸与血清TLR4、NF-κB、TNF-α和IL-6均呈负相关（P<0.05）。结论慢性荨麻疹患者血清炎症因子水平的升高可能与肠道产丁酸盐细菌丰度降低,导致肠道丁酸水平下降,上调了TLR4/NF-κB信号通路有关。

关键词: 肠道菌群, 短链脂肪酸, Toll样受体4, 肿瘤坏死因子α, 白细胞介素6, 慢性荨麻疹

Abstract:

Objective To investigate the influence of intestinal flora on serum inflammatory factors in patients with chronic urticaria. Methods Totally,60 patients with chronic urticaria and 60 healthy subjects were enrolled,and fasting venous blood and stool samples were collected. Gene 16S rDNA real-time fluorescence quantitative polymerase chain reaction（PCR） was used to determine the levels of Faecalibacterium prausnitzii,Clostridium leptum,Bifidobacterium and Lactobacillus. Gas chromatography was used to determine intestinal short chain fatty acid. Serum Toll-like receptor 4（TLR4）,nuclear factor（NF）-κB,tumor necrosis factor-alpha（TNF-α） and interleukin-6（IL-6） were determined by enzyme-linked immunosorbent assay（ELISA）. Spearman correlation analysis was used to analyze the relationship between intestinal flora,short chain fatty acid and serum TLR4,NF-κB,TNF-α and IL-6. Results Compared with the control group,the levels of Faecalibacterium prausnitzii,Clostridium leptum,Lactobacillus and butyric acid in the intestine of patients with chronic urticaria were decreased（P<0.05）. Serum TLR4,NF-κB,TNF-α and IL-6 levels were increased（P<0.05）. Correlation analysis showed that Faecalibacterium prausnitzii,Clostridium leptum,Lactobacillus and butyric acid were negatively correlated with serum TLR4,NF-κB,TNF-α and IL-6（P<0.05）. Conclusions The increase of serum inflammatory factors in patients with chronic urticaria may be related to the decrease of intestinal butyric acid-producing bacteria,leading to a decrease in intestinal butyric acid levels and up-regulation of TLR4/NF-κB signaling pathway.

Key words: Intestinal flora, Short chain fatty acid, Toll-like receptor 4, Tumor necrosis factor-alpha, Interleukin-6, Chronic urticaria

中图分类号:

R446.5

任媛, 张敏, 殷翔, 曾倩雯. 肠道菌群对慢性荨麻疹患者血清炎症因子的影响[J]. 检验医学, 2021, 36(5): 504-509.

REN Yuan, ZHANG Min, YIN Xiang, ZENG Qianwen. Influence of intestinal flora on serum inflammatory factors in patients with chronic urticaria[J]. Laboratory Medicine, 2021, 36(5): 504-509.

图/表 5

参考文献 25

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指标	TLR4		NF-κB		TNF-α		IL-6
指标	r值	P值	r值	P值	r值	P值	r值	P值
普拉梭菌	-0.813	0.004	-0.773	0.009	-0.721	0.019	-0.705	0.023
柔嫩梭菌	-0.650	0.042	-0.749	0.013	-0.787	0.007	-0.698	0.025
双歧杆菌	0.214	0.553	0.140	0.699	-0.061	0.868	0.041	0.911
乳酸杆菌	-0.724	0.018	-0.648	0.043	-0.620	0.056	-0.727	0.017
乙酸	0.019	0.959	-0.104	0.774	-0.328	0.356	-0.396	0.257
丙酸	-0.246	0.494	-0.159	0.662	-0.024	0.948	0.273	0.445
丁酸	-0.739	0.015	-0.876	0.001	-0.661	0.038	-0.760	0.011

指标	TLR4		NF-κB		TNF-α		IL-6
指标	r值	P值	r值	P值	r值	P值	r值	P值
普拉梭菌	-0.813	0.004	-0.773	0.009	-0.721	0.019	-0.705	0.023
柔嫩梭菌	-0.650	0.042	-0.749	0.013	-0.787	0.007	-0.698	0.025
双歧杆菌	0.214	0.553	0.140	0.699	-0.061	0.868	0.041	0.911
乳酸杆菌	-0.724	0.018	-0.648	0.043	-0.620	0.056	-0.727	0.017
乙酸	0.019	0.959	-0.104	0.774	-0.328	0.356	-0.396	0.257
丙酸	-0.246	0.494	-0.159	0.662	-0.024	0.948	0.273	0.445
丁酸	-0.739	0.015	-0.876	0.001	-0.661	0.038	-0.760	0.011

变量	β值	标准误	Wald值	P值	OR值	95%可信区间
普拉梭菌	-2.085	0.730	8.165	0.004	0.124	0.030~0.520
柔嫩梭菌	-1.886	0.772	5.962	0.015	0.152	0.033~0.689
双歧杆菌	0.499	0.748	0.446	0.504	1.648	0.381~7.131
乳酸杆菌	-2.260	0.970	5.430	0.020	0.104	0.016~0.698
乙酸	-0.023	0.023	0.977	0.323	0.978	0.935~1.022
丙酸	-0.165	0.111	2.217	0.136	0.848	0.683~1.053
丁酸	-0.304	0.127	5.733	0.017	0.738	0.575~0.946

变量	β值	标准误	Wald值	P值	OR值	95%可信区间
普拉梭菌	-2.085	0.730	8.165	0.004	0.124	0.030~0.520
柔嫩梭菌	-1.886	0.772	5.962	0.015	0.152	0.033~0.689
双歧杆菌	0.499	0.748	0.446	0.504	1.648	0.381~7.131
乳酸杆菌	-2.260	0.970	5.430	0.020	0.104	0.016~0.698
乙酸	-0.023	0.023	0.977	0.323	0.978	0.935~1.022
丙酸	-0.165	0.111	2.217	0.136	0.848	0.683~1.053
丁酸	-0.304	0.127	5.733	0.017	0.738	0.575~0.946