检验医学 ›› 2015, Vol. 30 ›› Issue (2): 145-148.DOI: 10.3969/j.issn.1673-8640.2015.02.010

• 临床应用研究·论著 • 上一篇    下一篇

不同临床分期HIV-1感染者/AIDS患者血样的蛋白印迹试验带型分析

郭川, 纪琳莹, 吴耀波, 林洁敏, 陈婉   

  1. 汕头市疾病预防控制中心卫生检验科,广东 汕头 515041
  • 收稿日期:2014-03-10 出版日期:2015-02-28 发布日期:2015-02-12
  • 作者简介:null

    作者简介:郭川,男,1981年生,硕士,主管技师,主要从事病原微生物研究。

  • 基金资助:
    汕头市医疗科技计划项目(汕府科[2013]88号)

The band patterns of Western blotting in different clinical stages of HIV-1 infection/AIDS patients

GUO Chuan, JI Linying, WU Yaobo, LIN Jiemin, CHEN Wan   

  1. Department of Healthy Laboratory, Center for Disease Control and Prevention of Shantou, Guangdong Shantou 515041, China
  • Received:2014-03-10 Online:2015-02-28 Published:2015-02-12

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摘要： 目的

分析各临床分期的人类免疫缺陷病毒1型(HIV-1)感染者/获得性免疫缺陷综合征(AIDS)患者血清中病毒蛋白抗体的变化,探讨不同临床分期的HIV-1感染者/AIDS患者蛋白印迹试验(WB)的带型特点。

方法

对汕头市2009至2012年确证的部分HIV-1抗体阳性病例进行CD4+T细胞计数,根据计数结果对病例进行分组:原发感染期组(CD4+ ≥500/mm3);感染中期组(CD4+ ≥ 200 /mm3且<500/mm3); AIDS期组(CD4+ <200/mm3);对各组的WB结果进行病毒蛋白(p17、p24、p31、p39、gp41、p51、p55、p66、gp120、gp160)抗体的阳性率统计以及带型分析。

结果

病毒蛋白p24、p31、gp41、p51、p66、gp120和gp160的抗体阳性率较高(总阳性率分别为98.2%、92.0%、90.8%、89.5%、93.3%、98.7%和97.5%),且在3个分期组间未见明显变化(P>0.05);病毒蛋白p17、p39和p55呈现较低的抗体阳性率(总阳性率分别为68.9%、49.1%和42.5%);蛋白p17的阳性率从原发感染期的91.5%降为感染中期的62.8%(P<0.01)及AIDS期59.3%(P<0.01);WB结果中的全带型、缺失p39+p55和缺失p17+p39+p55的带型是3个疾病分期组患者的常见带型;在3个分期组中,原发感染期中全带型出现率最高(60.8%),而缺失p17+p39+p55的出现率最低(6.5%)。

结论

病毒蛋白p17抗体的阴转可以作为疾病从原发感染期进入感染中期/AIDS期的一个潜在判别指标。

关键词: 带型分析, 蛋白印迹试验, 病毒抗体, 阳性率, 人类免疫缺陷病毒, 临床分期

Abstract: Objective

To analyze the change of anti-virus antibodies in serum of patients with different clinical stages of human immunodeficiency virus 1 (HIV-1) infection/ acquired immune deficiency syndrome (AIDS), and to investigate the band pattern characteristics of Western blotting (WB).

Methods

CD4+ T cell counting was determined in the part of HIV-1 confirmed cases in Shantou from 2009 to 2012. According to the results, these cases were classified into 3 groups, primary-stage infection group (CD4+ ≥500 /mm3), middle-stage infection group (CD4+ ≥ 200/mm3 and CD4+ <500 /mm3) and AIDS group (CD4+ <200 /mm3). The WB results of the 3 groups were analyzed and performed to determine the positive rates of antibodies against viral proteins (p17, p24, p31, p39, gp41, p51, p55, p66, gp120 and gp160) and the band patterns of WB.

Results

The antibodies against viral protein p24, p31, gp41, p51, p66, gp120 and gp160 showed high total positive rates (98.2%, 92.0%, 90.8%, 89.5%, 93.3%, 98.7% and 97.5%, respectively) , while the positive rates had no obvious difference among the 3 stages (P>0.05). The antibodies against viral proteins p17, p39 and p55 showed low positive rates (68.9%, 49.1% and 42.5%, respectively). The positive rate of protein p17 showed an obvious declining between primary-stage infection groups (91.5%) and middle-stage infection group 62.8% (P<0.01)as well as AIDS group 59.3% (P<0.01). All bands, missing p39+p55 and missing p17+p39+p55 were the common band patterns of WB among the 3 groups. Primary-stage infection group showed the highest appearance rate (60.8%) of all bands but the lowest appearance rate (6.5%) of missing p17+p39+p55.

Conclusions

Turning negative of antibody against viral protein p17 could be used as a potential distinguish parameter to forecast the disease progression of HIV-1 from primary-stage infection to middle-stage infection group/AIDS group.

Key words: Band pattern, Western blotting, Viral antibody, Positive rate, Human immunodeficiency virus, Clinical stage

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