检验医学 ›› 2013, Vol. 28 ›› Issue (3): 229-232.DOI: 10.3969/j.issn.1673-8640.2013.03.016

• 技术研究与评价.论著 • 上一篇    下一篇

两种电泳方法血红蛋白分析一致性的比较

陈永秀   

  1. 1.横县人民医院检验科，广西 横县 530300；2.横县人民医院产科，广西 横县 530300；3.横县人民医院血液科，广西 横县 530300；4.广西医科大学第一附属医院血红蛋白研究室，广西 南宁 530021
  • 收稿日期:2012-12-31 修回日期:2012-05-07 出版日期:2013-03-30 发布日期:2013-03-14
  • 作者简介:陈永秀，女，1968年生，主管技师，主要从事临床地中海贫血筛查工作。

Comparison on the consistency of two electrophoresis methods in the detection of hemoglobin

  1. 1.Department of Clinical Laboratory，Hengxian People′s Hospital，Guangxi Hengxian 530300，China；2. Department of Obstetrics，Hengxian People′s Hospital，Guangxi Hengxian 530300，China；3. Department of Hematology，Hengxian People′s Hospital，Guangxi Hengxian 530300，China；4.Hemoglobin Laboratory，the First Affiliated Hospital，Guangxi Medical University，Guangxi Nanning 530021，China
  • Received:2012-12-31 Revised:2012-05-07 Online:2013-03-30 Published:2013-03-14

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摘要： 目的　比较琼脂糖凝胶电泳与醋酸纤维素薄膜电泳血红蛋白检测结果的一致性。  方法　同时采用Sebia Hydrasys LC全自动琼脂糖凝胶电泳系统和醋酸纤维素薄膜电泳（pH值8.6）对4 126例进行地中海贫血筛查的样本进行血红蛋白各组分的检测。用分光光度计比色测定HbA2、HbH、HbBart′s含量，用碱变性试验检测HbF含量。对2种电泳方法的检测结果进行对比，不一致的样本采用基因分析确诊。 结果　4 126例样本中琼脂糖凝胶电泳共检出β-地中海贫血686例（HbA2和/或HbF增高）、α-地中海贫血321例（HbA2降低）；醋酸纤维素薄膜电泳检出β-地中海贫血683例、α-地中海贫血 323例。2种电泳方法对HbA2和HbF区带的检测能力基本一致。琼脂糖凝胶电泳检出HbH 103例、HbBart′s 71例、HbCS 63例；醋酸纤维素薄膜电泳检出HbH 141例、Hb Bart′s 101例、HbCS 65例。经基因分析确诊，琼脂糖凝胶电泳漏检HbH 38例、HbBart′s 30例、HbCS 2例。 结论　琼脂糖凝胶电泳可准确检出HbA2和HbF区带，对HbH、HbBart′s区带的检测能力不足。当HbA2降低、异丙醇试验阳性、红细胞脆性试验（一管法）阳性时需用醋酸纤维素薄膜电泳进行检测并定量测定HbH、Hb Bart′s值。在筛查和普查地中海贫血时可使用电泳方法，但在临床确诊和产前诊断时必须采用基因分析。

关键词: 血红蛋白, 琼脂糖凝胶电泳, 醋酸纤维素薄膜电泳, 地中海贫血

Abstract: Objective　To compare the results′consistency of agarose gel electrophoresis and cellulose acetate membrane electrophoresis in the detection of hemoglobin.  Methods　By Sebia Hydrasys LC automated agarose gel electrophoresis and cellulose acetate membrance electrophoresis，the hemoglobin levels of 4 126 patients with Mediterranean anaemia were detected（pH value ＝8.6）. The HbA2，HbH，and HbBart′s contents were determined by spectrophotometry. The HbF content was determined by alkaline denaturation test. The results of the 2 methods were compared. The cases with different results by the 2 methods were confirmed by genetic analysis.  Results　A total of 4 126 cases by agarose gel electrophoresis had 686 cases of beta-Mediterranean anaemia （HbA2 and/or HbF increasing）and 321 cases of alpha-Mediterranean anaemia（HbA2 decreasing）. Cellulose acetate membrane electrophoresis had 683 cases of beta-Mediterranean anaemia and 323 cases of alpha-Mediterranean anaemia. Basically，the results were consistent forHbA2 and HbF by the 2 methods. Agarose gel electrophoresis detected 103 cases of HbH，71 cases of HbBart′s and 63 cases of HbCS. Cellulose acetate membrane electrophoresis detected 141 cases of HbH，101 cases of HbBart′s and 65 cases of HbCS. After being confirmed by genetic analysis，agarose gel electrophoresis missed 38 cases of HbH，30 cases of HbBart′s and 2 cases of HbCS.  Conclusions　Agarose gel electrophoresis can accurately detect HbA2 and HbF，and it has deficiencies for HbH and HbBart′s. While HbA2 decreasing，isopropyl alcohol-test positive and erythrocyte fragility test （one tube method） positive，cellulose acetate membrane electrophoresis should be used to the quantitive determination of HbH and HbBart′s. Electrophoresis can be used in the screening and general investigation of Mediterranean anaemina，and genetic analysis should be used in clinical confirmation and antepartum diagnosis.

Key words: Hemoglobin, Agarose gel electrophoresis, Cellulose acetate membrane electrophoresis, Mediterranean anaemia

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