自贡地区表观健康成人血清PGⅠ、PGⅡ和PGR参考区间的建立

doi:10.3969/j.issn.1673-8640.2022.01.004

摘要/Abstract

摘要：

目的建立自贡地区表观健康成人血清胃蛋白酶原Ⅰ（PGⅠ）、胃蛋白酶原Ⅱ（PGⅡ）和胃蛋白酶原Ⅰ/胃蛋白酶原Ⅱ比值（PGR）的参考区间,为临床相关疾病诊治提供依据。方法选取自贡市第一人民医院表观健康体检者2 880名,采用胶乳增强免疫比浊法检测PGⅠ、PGⅡ水平,并计算PGR,同时采用¹³C尿素呼气试验筛查幽门螺杆菌（HP）的感染情况。采用百分位数法（P_2.5~P_97.5）建立参考区间。采用受试者工作特征（ROC）曲线评价PGⅠ、PGⅡ判断HP阳性的效能。采用Kendall's tau-b相关分析评价 PGⅠ、PGⅡ与HP阳性的相关性。结果男性血清PGⅠ、PGⅡ水平均高于女性（P<0.05）,PGR低于女性（P<0.05）。18~44岁组、45~59岁组、>59岁组血清PGⅠ和PGⅡ水平依次升高,各组间差异均有统计学意义（P<0.05）;各年龄组PGR差异均无统计学意义（P>0.05）。血清PGⅠ、PGⅡ根据性别和年龄分别建立参考区间,PGR按性别建立参考区间。以¹³C尿素呼气试验阳性作为HP阳性的依据,ROC曲线分析结果显示,PGⅠ、PGⅡ和PGR判断HP阳性的曲线下面积分别为0.712、0.825和0.799。相关性分析结果显示,PGⅠ、PGⅡ与HP阳性均呈正相关（r值分别为0.286、0.440,P<0.01）,PGR与HP阳性呈负相关（r=-0.403,P<0.01）。结论初步建立了自贡地区表观健康成人血清PGⅠ、PGⅡ及PGR的参考区间。

关键词: 胃蛋白酶原Ⅰ, 胃蛋白酶原Ⅱ, 参考区间

Abstract:

Objective To establish the reference intervals of serum pepsinogenⅠ（PGⅠ）, pepsinogenⅡ（PGⅡ） and the ratio of pepsinogenⅠ/pepsinogenⅡ（PGR） for apparently healthy subjects in Zigong,and to provide a reference for clinical diagnosis and treatment. Methods The levels of PGⅠ and PGⅡ were determined by latex enhanced immunoturbidimetry,¹³C urea breath test was used to screen Helicobacter pylori（HP） infection in 2 880 apparently healthy subjects from Zigong First People's Hospital,and PGR was calculated. The reference interval was established by percentile method（P_2.5-P_97.5）. The efficacy of PGⅠand PG Ⅱin judging HP positivity was evaluated by receiver operating characteristic（ROC） curve. Kendall's tau-b correlation analysis was used to evaluate the correlation between PGⅠ,PGⅡ and HP positivity. Results The levels of serum PGⅠand PGⅡin males were higher than those in females（P<0.05）,and PGR was lower（P<0.05）. The levels of serum PGⅠand PGⅡ in 18-44-year-old group,45-59-year-old group and >59-year-old group increased in turn（P<0.05）. There was no statistical significance for PGR among the age groups（P>0.05）. The reference intervals of serum PGⅠand PGⅡ were established according to sex and age,and the reference intervals of PGR were established according to sex. Taking the positive ¹³C urea breath test as the basis for HP positivity,the areas under curves for HP positivity judged by PGⅠ,PGⅡ and PGR were 0.712,0.825 and 0.799,respectively. There were correlations between PGⅠ,PGⅡ and HP positivity（r=0.286 and 0.440,respectively,P<0.01）,while PGR was negatively correlated with HP positivity（r=-0.403,P<0.01）. Conclusions The reference intervals of serum PGⅠ,PGⅡ and PGR in apparently healthy subjects in Zigong have been established.

Key words: Pepsinogen Ⅰ, Pepsinogen Ⅱ, Reference interval

中图分类号:

R446.1

余建洪, 彭庆远, 殷明刚. 自贡地区表观健康成人血清PGⅠ、PGⅡ和PGR参考区间的建立[J]. 检验医学, 2022, 37(1): 23-27.

YU Jianhong, PENG Qingyuan, YIN Minggang. Establishment of reference intervals of serum PGⅠ,PGⅡ and PGR for apparently healthy subjects in Zigong[J]. Laboratory Medicine, 2022, 37(1): 23-27.

图/表 6

参考文献 19

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性别	例数	PGⅠ/（ng/mL）	PGⅡ/（ng/mL）	PGR
男性	1 804	63.20（50.70~77.80）	14.30（9.70~23.70）	4.68（2.93~5.87）
女性	1 076	50.90（41.43~64.55）	10.70（7.63~17.20）	4.92（3.71~5.93）
Z值		-6.941	-5.781	-2.207
P值		0.000	0.000	0.027

性别	例数	PGⅠ/（ng/mL）	PGⅡ/（ng/mL）	PGR
男性	1 804	63.20（50.70~77.80）	14.30（9.70~23.70）	4.68（2.93~5.87）
女性	1 076	50.90（41.43~64.55）	10.70（7.63~17.20）	4.92（3.71~5.93）
Z值		-6.941	-5.781	-2.207
P值		0.000	0.000	0.027

组别	例数	PGⅠ/（ng/mL）	PGⅡ/（ng/mL）	PGR
18~44岁组	1 468	52.30（43.00~67.30）	10.90（7.90~19.20）	4.94（3.36~5.91）
45~59岁组	1 160	63.80（50.78~84.10）^*	14.00（10.00~23.70）^*	4.59（3.09~5.90）
>59岁组	252	66.95（56.20~87.88）^*#	15.95（11.48~23.20）^*#	4.70（3.47~5.52）
H值		54.574	26.212	2.073
P值		0.000	0.000	0.355

组别	例数	PGⅠ/（ng/mL）	PGⅡ/（ng/mL）	PGR
18~44岁组	1 468	52.30（43.00~67.30）	10.90（7.90~19.20）	4.94（3.36~5.91）
45~59岁组	1 160	63.80（50.78~84.10）^*	14.00（10.00~23.70）^*	4.59（3.09~5.90）
>59岁组	252	66.95（56.20~87.88）^*#	15.95（11.48~23.20）^*#	4.70（3.47~5.52）
H值		54.574	26.212	2.073
P值		0.000	0.000	0.355

组别	例数	PGⅠ/（ng/mL）	PGⅡ/（ng/mL）	PGR
18~44岁组
男性	936	57.70（47.08~71.73）	13.25（9.18~21.43）	4.83（2.99~5.85）
女性	532	43.50（38.35~54.35）^△	8.50（6.75~14.75）^△	5.22（3.98~6.08）^△
45~59岁组
男性	724	67.30（53.75~86.25）^*	15.10（10.30~26.15）^*	4.57（2.71~6.09）
女性	436	58.70（47.60~76.35）^*△	12.10（9.30~20.20）^*△	4.66（3.56~5.73）
>59岁组
男性	144	69.60（58.53~94.33）^*#	16.70（13.90~23.85）^*#	4.18（3.46~5.29）
女性	108	57.35（51.95~77.20）^*#	11.35（9.80~22.30）^*#△	4.84（4.21~5.89）