哮喘患儿支气管肺泡灌洗液GM-CSF和MPO水平的变化及意义

doi:10.3969/j.issn.1673-8640.2017.07.010

摘要/Abstract

摘要：

目的探讨哮喘患儿支气管肺泡灌洗液（BALF）粒细胞-巨噬细胞集落刺激因子（GM-CSF）和髓过氧化物酶（MPO）在哮喘发生、发展中的作用。方法收集29例哮喘患儿哮喘发作期和缓解期以及25例对照患儿的BALF样本,在显微镜下计数中性粒细胞（NEU）百分率,采用酶联免疫吸附试验检测GM-CSF和MPO水平。结果哮喘组哮喘发作期和缓解期及对照组BALF GM-CSF的水平分别为（164.8±34.0）、（129.5±30.4）和（123.0±25.7）ng/L,MPO水平分别为（25.1±9.7）、（15.5±4.4）和（13.8±3.5） ng/ L,NEU百分率分别为（35.1±7.6）%、（26.3±6.2）%和（23.8±5.5）%。哮喘组哮喘发作期BALF GM-CSF和MPO水平及NEU百分率均高于哮喘缓解期和对照组（P<0.01）,而哮喘组哮喘缓解期BALF GM-CSF和MPO水平及NEU百分率与对照组比较差异均无统计学意义（P>0.05）。哮喘组哮喘发作期GM-CSF水平与NEU百分率、MPO水平呈正相关（r 值分别为0.504、0.665,P <0.01）,NEU百分率与MPO水平呈正相关（r =0.669,P<0.01）。结论哮喘患儿BALF GM-CSF和MPO水平升高与疾病的发生、发展关系密切,BALF GM-CSF和MPO可作为评价和监测哮喘患儿病情的有效指标。

关键词: 粒细胞-巨噬细胞集落刺激因子, 髓过氧化物酶, 中性粒细胞百分率, 支气管肺泡灌洗液, 哮喘, 儿童

Abstract:

Objective To investigate the roles of granulocyte-macrophage colony-stimulating factor （GM-CSF）and myeloperoxidase （MPO） in bronchoalveolar lavage fluid（BALF）among asthmatic children. Methods A total of 29 asthmatic children and 25 controls were enrolled. By microscopy,the percentage of neutrophil（NEU）was determined. The levels of GM-CSF and MPO were determined by enzyme-linked immunosorbent assay. Results The levels of GM-CSF in BALF were （164.8±34.0） ng/L in attack stage group,（129.5±30.4） ng/L in remission stage group and（123.0±25.7）ng/L in control group. The levels of MPO were （25.1±9.7）,（15.5±4.4）and（13.8±3.5）ng/L in the 3 groups,respectively. The percentages of NEU were （35.1±7.6）%,（26.3±6.2）% and（23.8±5.5）% in the 3 groups,respectively. The levels of GM-CSF and MPO in BALF and the percentages of NEU were higher in attack stage group than those in remission stage and control groups（P<0.01）,but there was no statistical significance between remission stage and control groups （P>0.05）. GM-CSF level was correlated positively with MPO level and the percentage of NEU（r = 0.665 and 0.504,P<0.01）,and the percentage of NEU was correlated positively with MPO level（r = 0.669,P<0.01）in attack stage group. Conclusions Both GM-CSF and MPO levels increase among asthmatic children,and they might be involved in the pathological process of asthma. GM-CSF and MPO in BALF may be indices for evaluating and monitoring the status of children with asthma.

Key words: Granulocyte-macrophage colony-stimulating factor, Myeloperoxidase, Neutrophil percentage, Bronchoalveolar lavage fluid, Asthma, Children

中图分类号:

R446.62

徐庆雷, 周红, 马小波, 张巍, 刘玲玲, 刘刚, 张敏. 哮喘患儿支气管肺泡灌洗液GM-CSF和MPO水平的变化及意义[J]. 检验医学, 2017, 32(7): 603-606.

XU Qinglei, ZHOU Hong, MA Xiaobo, ZHANG Wei, LIU Lingling, LIU Gang, ZHANG Min. Changes of granulocyte-macrophage colony-stimulating factor and myeloperoxidase levels in bronchoalveolar lavage fluid among asthmatic children[J]. Laboratory Medicine, 2017, 32(7): 603-606.

图/表 2

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组别	例数	GM-CSF（ng/L）	MPO（ng/L）	NEU百分率（%）
哮喘组	29
哮喘发作期		164.8±34.0^*#	25.1±9.7^*#	35.1±7.6^*#
哮喘缓解期		129.5±30.4	15.5±4.4	26.3±6.2
对照组	25	123.0±25.7	13.8±3.5	23.8±5.5
F值		46.58	72.81	52.34
P值		0.000	0.000	0.000

组别	例数	GM-CSF（ng/L）	MPO（ng/L）	NEU百分率（%）
哮喘组	29
哮喘发作期		164.8±34.0^*#	25.1±9.7^*#	35.1±7.6^*#
哮喘缓解期		129.5±30.4	15.5±4.4	26.3±6.2
对照组	25	123.0±25.7	13.8±3.5	23.8±5.5
F值		46.58	72.81	52.34
P值		0.000	0.000	0.000