外周血DNT细胞及T细胞亚群变化与乙型肝炎慢性化的相关性研究

doi:10.3969/j.issn.1673-8640.2015.07.013

摘要/Abstract

摘要： 目的

探讨外周血中CD3⁺CD4^-CD8^-(DNT)细胞及T细胞亚群变化与乙型肝炎病毒(HBV)感染慢性化的关系。

方法

用流式细胞术测定136例HBV感染者[33例无症状携带者(ASC)、28例急性乙型肝炎(AHB)、28例轻度慢性乙型肝炎(CHB)、25例中度CHB、22例重度CHB患者]及39名健康对照(HC)外周血中的DNT细胞及T细胞亚群。

结果

ASC组、AHB组、HC组DNT细胞比例分别为5.43%±3.31%、4.75%±2.71%和4.82%±3.43%,3组间差异无统计学意义(P>0.05),其中HC组和AHB组均低于CHB各组(由轻到重分别为7.97%±4.12%、8.41%±4.93%、11.36%±5.01%,P均<0.05),但ASC组仅显著低于重度CHB组(P<0.05),与轻度CHB组和中度CHB组间差异均无统计学意义(P>0.05);DNT细胞比例在轻度CHB组与中度CHB组间差异无统计学意义(P>0.05),但二组均显著低于重度CHB组(P均<0.05)。HC组、AHB组、ASC组间T淋巴细胞亚群分布差异无统计学意义(P均>0.05),但随着CHB患者病情加重CD3⁺、CD3⁺CD4⁺CD8^-(CD4⁺)细胞比例降低,CD3⁺CD4^-CD8⁺(CD8⁺)细胞比例升高。

结论

外周血DNT细胞比例的升高与HBV感染者慢性化关系密切,且与CHB患者的疾病进程有关。

关键词: CD3+CD4-CD8-细胞, T淋巴细胞亚群, 急性乙型肝炎, 慢性乙型肝炎

Abstract: Objective

To investigate the changes of CD3⁺CD4^-CD8^-(DNT) cells and T lymphocyte subsets in the chronic progress of hepatitis B virus (HBV) intection.

Methods

DNT cells and T lymphocyte subsets of peripheral blood were detected by flow cytometry in 136 patients with HBV infection, including 33 cases of asymptomatic carrier(ASC), 28 cases of acute hepatitis B(AHB), 28 cases of mild chronic hepatitis B (CHB), 25 cases of moderate CHB, 22 cases of severe CHB, and 39 healthy controls (HC).

Results

DNT cell percentages in ASC, AHB and HC groups were 5.43%±3.31%, 4.75%±2.71% and 4.82%±3.43%, and there was no statistical significance among the 3 groups (P<0.05). DNT cell percentages in HC and AHB group were lower than that in CHB group (from mild group to severe group, DNT cell percentages were 7.97%±4.12%, 8.41%±4.93% and 11.36%±5.01%, P<0.05). The DNT percentage in ASC group was only lower than that in severe CHB group (P<0.05). The DNT percentage had no statistical significance in severe CHB group with those in mild CHB and moderate CHB groups (P>0.05). There was no statistical significance for DNT cell percentages between mild and moderate CHB groups (P>0.05), and they were significantly lower than that in severe group (P<0.05). There was no statistical significance for T lymphocyte subsets in HC group, AHB group and ASC group (P>0.05). With the development of CHB, the percentages of CD3⁺ and CD3⁺CD4⁺CD8^-(CD4⁺) cells decreased, and the percentages of CD3⁺CD4^-CD8⁺ (CD8⁺) cells increased.

Conclusions

The increasing of peripheral blood DNT cell percentage is associated with the progress of CHB.

Key words: CD3+CD4-CD8- cell, T lymphocyte subset, Acute hepatitis B, Chronic hepatitis B

中图分类号:

R446.63

杜振华, 鲍布和, 王仁杰, 任党利, 张明华, 刘冀琴. 外周血DNT细胞及T细胞亚群变化与乙型肝炎慢性化的相关性研究[J]. 检验医学, 2015, 30(7): 723-727.

DU Zhenhua, BAO Buhe, WANG Renjie, REN Dangli, ZHANG Minghua, LIU Jiqin. Correlation analysis on the changes of DNT cells and T lymphocyte subsets in the chronic progress of hepatitis B[J]. Laboratory Medicine, 2015, 30(7): 723-727.

图/表 3

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组别	例数(男/女)	年龄(岁)	HBsAg/HBeAg/抗HBc	ALT(IU/L)	AST(IU/L)
HC组	39(21/18)	42.00±7.52	-/-/-	38.00±8.92	39.00±7.40
ASC组	33(20/13)	46.00±9.15	+/-/-	39.00±9.11	37.00±10.05
CHB
轻度组	28(18/10)	43.00±8.53	+/+/+	73.00±12.94	69.00±12.44
中度组	25(15/10)	44.00±10.11	+/+/+	127.00±11.75	112.00±13.82
重度组	22(11/11)	45.00±6.93	+/+/+	401.00±13.56	345.00±16.13
AHB组	28(16/12)	47.00±8.22	+/+/+	843.00±123.62	796.00±158.71

组别	例数(男/女)	年龄(岁)	HBsAg/HBeAg/抗HBc	ALT(IU/L)	AST(IU/L)
HC组	39(21/18)	42.00±7.52	-/-/-	38.00±8.92	39.00±7.40
ASC组	33(20/13)	46.00±9.15	+/-/-	39.00±9.11	37.00±10.05
CHB
轻度组	28(18/10)	43.00±8.53	+/+/+	73.00±12.94	69.00±12.44
中度组	25(15/10)	44.00±10.11	+/+/+	127.00±11.75	112.00±13.82
重度组	22(11/11)	45.00±6.93	+/+/+	401.00±13.56	345.00±16.13
AHB组	28(16/12)	47.00±8.22	+/+/+	843.00±123.62	796.00±158.71

组别	CD3⁺细胞(%)	CD4⁺细胞(%)	CD8⁺细胞(%)	CD4⁺/CD8⁺
HC组	71.08±7.04	40.58±6.28	24.84±5.96	1.77±0.62
ASC组	70.79±8.92^△▲□	42.03±8.36^△▲□	24.61±4.56^△▲□	1.76±0.49^△▲□
CHB
轻度组	62.38±9.24^*#	33.57±8.36^*	30.29±8.91^*#	1.23±0.49^*#
中度组	57.66±9.97^*#	30.63±4.78^*#	36.02±8.12^*#△	0.93±0.31^*#△
重度组	43.92±8.72^*#△▲	26.1±5.21^*#△	42.63±7.08^*#△▲	0.65±0.19^*#△
AHB组	71.3±6.81	41.54±6.23	24.59±4.55	1.69±0.43

组别	CD3⁺细胞(%)	CD4⁺细胞(%)	CD8⁺细胞(%)	CD4⁺/CD8⁺
HC组	71.08±7.04	40.58±6.28	24.84±5.96	1.77±0.62
ASC组	70.79±8.92^△▲□	42.03±8.36^△▲□	24.61±4.56^△▲□	1.76±0.49^△▲□
CHB
轻度组	62.38±9.24^*#	33.57±8.36^*	30.29±8.91^*#	1.23±0.49^*#
中度组	57.66±9.97^*#	30.63±4.78^*#	36.02±8.12^*#△	0.93±0.31^*#△
重度组	43.92±8.72^*#△▲	26.1±5.21^*#△	42.63±7.08^*#△▲	0.65±0.19^*#△
AHB组	71.3±6.81	41.54±6.23	24.59±4.55	1.69±0.43