银屑病患者Th17和Treg细胞及其相关细胞因子的表达

doi:10.3969/j.issn.1673-8640.2015.07.011

检验医学 ›› 2015, Vol. 30 ›› Issue (7): 715-719.DOI: 10.3969/j.issn.1673-8640.2015.07.011

银屑病患者Th17和Treg细胞及其相关细胞因子的表达

杨婷婷¹, 王青¹, 李志¹, 朴君², 朴敬爱²

1. 大连市中心医院检验科,辽宁大连 116033
2.辽宁师范大学生命科学学院,辽宁大连 116033

收稿日期:2014-09-05 出版日期:2015-07-30 发布日期:2015-08-28
作者简介:null
作者简介:杨婷婷,女,1987年生,硕士,技师,主要从事临床检验工作。

通讯作者:李志,联系电话:0411-84412001。
基金资助:
大连市医药卫生科学研究项目

Study on Th17, Treg cell and the expression of relative cytokines in psoriasis patients

YANG Tingting¹, WANG Qing¹, LI Zhi¹, PIAO Jun², PIAO Jing'ai²

1.Department of Clinical Laboratory, Dalian Municipal Central Hospital, Liaoning Dalian 116033, China
2. Life Science College of Liaoning Normal University, Dalian Liaoning 116033, China

Received:2014-09-05 Online:2015-07-30 Published:2015-08-28

摘要/Abstract

摘要： 目的

探讨银屑病患者辅助性T细胞17(Th17)和调节性T (Treg)细胞及其相关因子表达的临床意义。

方法

用流式细胞术检测28例轻度和25例重度银屑病患者及25名健康对照者外周血中Th17和Treg细胞的比例;用逆转录聚合酶链反应(PCR)检测维甲酸相关孤儿受体γt(RORγt)和抗人叉头状转录因子(Foxp3) mRNA的表达;用酶联免疫吸附试验(ELISA)检测血浆Th17和Treg相关细胞因子白细胞介素17(IL-17)和转化生长因子β(TGF-β)的水平。

结果

与健康对照组比较,银屑病患者组Th17 细胞比例、RORγt的mRNA水平及IL-17的浓度显著上升,且重度组显著高于轻度组(P<0.05);而重度银屑病患者Treg细胞、Foxp3 mRNA和血浆TGF-β浓度均较健康对照组和轻度组显著降低(P<0.05)。

结论

Th17和Treg细胞的比例及其相关因子的表达可作为银屑病病情进展的标志。

关键词: 辅助性T细胞17, 调节性T细胞, 银屑病

Abstract: Objective

To investigate the clinical significance of T helper cell 17(Th17), regulatory T (Treg) cell and the expression of relative cytokines in psoriasis patients.

Methods

Flow cytometry was used to analyze the percentages of Th17 and Treg cells in 28 patients with mild psoriasis, 25 patients with severe psoriasis and 25 healthy controls. The mRNA expressions of retinoid-related orphan receptor gamma t (RORγt) and forkhead bos p3 (Foxp3) were detected by reverse transcription polymerase chain reaction (PCR). The concentrations of interleukin 17 (IL-17) and transforming growth facfor beta (TGF-β) in plasma of psoriasis patients were detected by enzyme-linked immunosorbent assay (ELISA).

Results

The percentages of Th17,RORγt mRNA and IL-17 in psoriasis patients increased compared with healthy controls,and there was a significantly higher levels in severe group than mild group (P<0.05). The percentage of Treg cells, Foxp3 mRNA and TGF-β in severe group were significantly lower than those in healthy controls and mild group(P<0.05).

Conclusions

The percentages of Th17 and Treg cells and their relative cytokines can be used as markers of psoriasis progression.

Key words: T helper cell 17, Regulatory T cell, Psoriasis

中图分类号:

R446.63

杨婷婷, 王青, 李志, 朴君, 朴敬爱. 银屑病患者Th17和Treg细胞及其相关细胞因子的表达[J]. 检验医学, 2015, 30(7): 715-719.

YANG Tingting, WANG Qing, LI Zhi, PIAO Jun, PIAO Jing'ai. Study on Th17, Treg cell and the expression of relative cytokines in psoriasis patients[J]. Laboratory Medicine, 2015, 30(7): 715-719.

图/表 5

参考文献 12

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组别	例数	IL-17(pg/mL)	TGF-β(pg/mL)
健康对照组	25	12.08±3.39	30.74±2.66
银屑病
轻度组	28	23.08±5.13^*	28.62±5.02
重度组	25	48.26±7.56^*#	20.60±2.61^*#

组别	例数	IL-17(pg/mL)	TGF-β(pg/mL)
健康对照组	25	12.08±3.39	30.74±2.66
银屑病
轻度组	28	23.08±5.13^*	28.62±5.02
重度组	25	48.26±7.56^*#	20.60±2.61^*#

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银屑病患者Th17和Treg细胞及其相关细胞因子的表达

Study on Th17, Treg cell and the expression of relative cytokines in psoriasis patients

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Metrics

组别	例数	RORγt	Foxp3
健康对照组	25	0.60±0.07	0.51±0.05
银屑病
轻度组	28	0.77±0.12^*	0.48±0.07
重度组	25	0.89±0.19^*#	0.43±0.08^*#