三维适形放射治疗联合化疗对食管癌患者外周血Treg的影响

doi:10.3969/j.issn.1673-8640.2017.05.010

检验医学 ›› 2017, Vol. 32 ›› Issue (5): 390-393.DOI: 10.3969/j.issn.1673-8640.2017.05.010

三维适形放射治疗联合化疗对食管癌患者外周血Treg的影响

徐鑫鑫¹, 王运刚², 蔡花¹, 沈培¹, 褚福营³

1. 南通大学附属医院检验科,江苏南通 226000
2. 江苏大学临床医学院,江苏镇江 212013
3. 南通市第一人民医院检验科,江苏南通 226000

收稿日期:2016-05-31 出版日期:2017-05-20 发布日期:2017-06-06
作者简介:null
作者简介：徐鑫鑫,女,1989年生,硕士,技师,主要从事临床检验工作。

通信作者：褚福营,联系电话：0513-85061219。

Influence of three dimensional conformal radiation therapy combined with chemotherapy on Treg in peripheral blood of esophagus carcinoma patients

XU Xinxin¹, WANG Yungang², CAI Hua¹, SHEN Pei¹, CHU Fuying³

1. Department of Clinical Laboratory,Affiliated Hospital of Nantong University,Nantong 226000,Jiangsu,China
2. Jiangsu University School of Medicine,Zhenjiang 212013,Jiangsu,China
3. Department of Clinical Laboratory,Nantong First People' s Hospital,Nantong 226000,Jiangsu,China

Received:2016-05-31 Online:2017-05-20 Published:2017-06-06

摘要/Abstract

摘要：

目的观察三维适形放射治疗（3D-CRT）联合化疗对食管癌患者外周血CD4⁺Foxp⁺调节性T细胞（Treg）的比例及转化生长因子β1（TGF-β1）水平的影响,探讨3D-CRT联合化疗治疗食管癌的内在机制。方法应用流式细胞术检测87例术后食管癌患者和30名健康者Treg比例,酶联免疫吸附试验（ELISA）检测血清TGF-β1水平,分析3D-CRT联合化疗患者、3D-CRT患者及健康者外周血Treg比例和TGF-β1水平差异。结果放化疗后,3D-CRT联合化疗组患者外周血单个核细胞（PBMC）中Treg占CD4⁺T细胞的比例及血清TGF-β1水平较3D-CRT组显著下降（P<0.05）。结论 3D-CRT联合化疗能有效下调食管癌患者外周血Treg比例和血清TGF-β1水平,对食管癌治疗具有重要意义。

关键词: 调节性T细胞, 转化生长因子β1, 三维适形放射治疗, 化疗, 食管癌

Abstract:

Objective To investigate the influence of three dimensional conformal radiation therapy （3D-CRT） combined with chemotherapy on CD4⁺Foxp⁺ regulatory T cell （Treg） proportion and transforming growth factor-beta 1 （TGF-β1） level in peripheral blood of esophagus carcinoma patients,and to investigate the internal influence mechanism of 3D-CRT combined with chemotherapy for the treatment of esophagus carcinoma. Methodse Flow cytometry was used to determine Treg proportion in 87 esophagus carcinoma patients and 30 healthy subjects. Serum TGF-β1 level was determined by enzyme-linked immunosorbent assay （ELISA）. The difference of Treg proportion and TGF-β1 level was analyzed among patients undergoing 3D-CRT combined with chemotherapy,patients with single 3D-CRT and healthy subjects. Results Compared to single 3D-CRT group,the Treg proportion and TGF-β1 level in peripheral blood decreased in 3D-CRT combined with chemotherapy group after patients undergoing radiochemotherapy （P<0.05）. Conclusions The 3D-CRT combined with chemotherapy could reduce Treg proportion and TGF-β1 level in peripheral blood of esophagus carcinoma patients,and plays a role for the treatment of esophagus carcinoma.

Key words: Regulatory T cell, Transforming growth factor-beta 1, Three dimensional conformal radiation therapy, Chemotherapy, Esophagus carcinoma

中图分类号:

R446.63

徐鑫鑫, 王运刚, 蔡花, 沈培, 褚福营. 三维适形放射治疗联合化疗对食管癌患者外周血Treg的影响[J]. 检验医学, 2017, 32(5): 390-393.

XU Xinxin, WANG Yungang, CAI Hua, SHEN Pei, CHU Fuying. Influence of three dimensional conformal radiation therapy combined with chemotherapy on Treg in peripheral blood of esophagus carcinoma patients[J]. Laboratory Medicine, 2017, 32(5): 390-393.

图/表 2

参考文献 13

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组别	例数	Treg占CD4⁺ T细胞的比例（%）		TGF-β1水平（ng/L）
组别	例数	放化疗前	放化疗后	放化疗前	放化疗后
正常对照组	30	1.35±1.32	1.35±1.32	49.70±6.20	49.70±6.20
3D-CRT组	39	6.30±1.73^**	4.20±1.78^**	134.70±15.47^**	76.40±5.48^*
3D-CRT联合化疗组	48	5.80±1.64^**	2.20±1.81^{* #}	138.30±18.95^**	54.30±4.81^#

组别	例数	Treg占CD4⁺ T细胞的比例（%）		TGF-β1水平（ng/L）
组别	例数	放化疗前	放化疗后	放化疗前	放化疗后
正常对照组	30	1.35±1.32	1.35±1.32	49.70±6.20	49.70±6.20
3D-CRT组	39	6.30±1.73^**	4.20±1.78^**	134.70±15.47^**	76.40±5.48^*
3D-CRT联合化疗组	48	5.80±1.64^**	2.20±1.81^{* #}	138.30±18.95^**	54.30±4.81^#

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三维适形放射治疗联合化疗对食管癌患者外周血Treg的影响

Influence of three dimensional conformal radiation therapy combined with chemotherapy on Treg in peripheral blood of esophagus carcinoma patients

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