活动性肺结核患者外周血产IL-37b淋巴细胞比例

doi:10.3969/j.issn.1673-8640.2017.05.011

检验医学 ›› 2017, Vol. 32 ›› Issue (5): 394-398.DOI: 10.3969/j.issn.1673-8640.2017.05.011

活动性肺结核患者外周血产IL-37b淋巴细胞比例

增加及其临床意义

刘淦斌¹,张俊爱²,代友超², 庄泽岗³,陈晨³,易来龙¹,徐军发²（1.东莞市第六人民医院,广东东莞 523122
2.广东医科大学检验医学研究所,广东东莞 523808
3.广东省医学分子诊断重点实验室,广东东莞 523808

收稿日期:2016-06-14 出版日期:2017-05-20 发布日期:2017-06-06
作者简介:null
作者简介：刘淦斌,男,1981年生,学士,主治医师,主要从事结核免疫研究。

通信作者：徐军发,联系电话：0769-22896372。
基金资助:
国家自然科学基金面上项目（81570009、81273237）;广东省自然科学基金项目（2015A030313513）;广东医科大学面上培育项目（2XK14058）

Elevation of interleukin-37b-producing lymphocyte proportion in patients with active pulmonary tuberculosis

LIU Ganbin¹, ZHANG Junai², DAI Youchao², ZHUANG Zegang³, CHEN Chen³, YI Lailong¹, XU Junfa²

1. Dongguan Sixth People's Hospital,Dongguan 523122,Guangdong,China
2. Institute of Laboratory Medicine,Guangdong Medical University,Dongguan 523808,Guangdong,China
3. Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics,Dongguan 523808,Guangdong,China

Received:2016-06-14 Online:2017-05-20 Published:2017-06-06

摘要/Abstract

摘要：

目的检测活动性肺结核（ATB）患者外周血产白细胞介素37b（IL-37b）淋巴细胞比例,探讨其临床意义。方法采用流式细胞术对42例ATB患者外周血产IL-37b淋巴细胞进行检测,并对8例患者进行治疗后跟踪检测,以10名健康志愿者作为对照组。结果 ATB患者外周血产IL-37b淋巴细胞比例明显高于对照组,8例跟踪患者治疗后产IL-37b淋巴细胞比例显著下降;结核抗体阳性患者外周血产IL-37b淋巴细胞比例高于结核抗体阴性患者;痰涂片阳性患者产IL-37b淋巴细胞比例显著高于痰涂片阴性患者;复治ATB患者产IL-37b淋巴细胞比例显著高于初治患者。体外卡介苗刺激可致人外周血单个核细胞（PBMC）中产IL-37b淋巴细胞比例显著增加,且在ATB组增加更显著。结论 ATB患者外周血中产IL-37b淋巴细胞比例增加与ATB的严重程度及预后相关,检测外周血产IL-37b淋巴细胞比例有可能成为ATB疗效评估的指标。

关键词: 白细胞介素37b, 淋巴细胞, 流式细胞术, 活动性肺结核

Abstract:

Objective To investigate the proportion of interleukin （IL）-37b-producing lymphocytes in patients with active pulmonary tuberculosis （ATB）. Methodse IL-37b-producing lymphocytes in peripheral blood from 42 patients with ATB were determined by flow cytometry,and the 8 patients were followed up. A total of 10 healthy subjects were enrolled as healthy control group. Results The proportion of IL-37b-producing lymphocytes in patients with ATB was higher than that in healthy control group,and it was decreased after treatment in the 8 followed-up patients. The proportion of IL-37b-producing lymphocytes in patients with positive tuberculosis antibody and positive sputum smear were higher than those in patients with negative tuberculosis antibody and negative sputum smear,respectively. The proportion of IL-37b-producing lymphocytes in patients received treatment again was higher than that in patients received initial treatment. The proportion of IL-37b-producing lymphocytes in peripheral blood mononuclear cell （PBMC）increased after the stimulation by bacillus Calmette-Guerin vaccination,especially for ATB patients. Conclusions The proportion of IL-37b-producing lymphocytes in patients with ATB is correlated with the severity and prognosis of ATB. The proportion of IL-37b-producing lymphocytes might be a parameter for evaluating the curative effect in ATB.

Key words: Interleukin-37b, Lymphocytes, Flow cytometry, Active pulmonary tuberculosis

中图分类号:

R446.62

增加及其临床意义. 活动性肺结核患者外周血产IL-37b淋巴细胞比例[J]. 检验医学, 2017, 32(5): 394-398.

LIU Ganbin, ZHANG Junai, DAI Youchao, ZHUANG Zegang, CHEN Chen, YI Lailong, XU Junfa. Elevation of interleukin-37b-producing lymphocyte proportion in patients with active pulmonary tuberculosis[J]. Laboratory Medicine, 2017, 32(5): 394-398.

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参考文献 16

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活动性肺结核患者外周血产IL-37b淋巴细胞比例

Elevation of interleukin-37b-producing lymphocyte proportion in patients with active pulmonary tuberculosis

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