HBV感染者外周血Th17细胞和Treg细胞的检测及意义

doi:10.3969/j.issn.1673-8640.2014.01.005

检验医学 ›› 2014, Vol. 29 ›› Issue (1): 21-25.DOI: 10.3969/j.issn.1673-8640.2014.01.005

• 乙型肝炎病毒感染者细胞免疫的临床监测专题 • 上一篇下一篇

HBV感染者外周血Th17细胞和Treg细胞的检测及意义

胡月明, 马莉, 李虹

贵阳医学院附属医院中心实验室, 贵州贵阳 550004

收稿日期:2013-05-07 出版日期:2014-01-20 发布日期:2014-01-20
通讯作者: 马　莉, 联系电话:0851-6774109。
作者简介:胡月明, 女, 1986年生, 硕士, 主要从事临床检验工作。
基金资助:
贵州省科技厅资助项目(黔科合LG字[2011]034号)

The detections of peripheral blood Th17 cells and Treg cells in HBV infected patients and their significance

HU Yueming, MA Li, LI Hong.

Central Laboratory, the Affiliated Hospital of Guiyang Medical College, Guizhou Guiyang 550004, China

Received:2013-05-07 Online:2014-01-20 Published:2014-01-20

摘要/Abstract

摘要：

目的探讨乙型肝炎病毒(HBV)感染者外周血中辅助性T细胞17(Th17)细胞和调节性T细胞(Treg)的比例变化及其与HBV感染后肝脏损伤的关系。方法采用流式细胞术检测34例乙型肝炎病毒携带者(ASC)、50例慢性乙型肝炎(CHB)患者、32例肝细胞癌(HCC)伴活动性肝炎(简称HCC)患者及30名健康对照者(正常对照组)外周全血Th17细胞和Treg细胞, 同时采用西门子1650全自动生化分析仪检测血清丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)。结果与正常对照组的Th17细胞比例(1.13%±0.37%)比较, ASC组略有升高(1.30%±0.28%), 但差异无统计学意义(P>0.05);CHB组(2.06%±1.15%)和HCC组(5.89%±3.31%)均明显升高(P<0.01), 且HCC组高于CHB组(P<0.05)。Th17细胞比例与ALT、AST呈正相关[相关系数(r)分别为0.682、0.637, P均＜0.01]。与正常对照组CD4⁺CD25⁺CD127^lowTreg细胞(7.18%±2.28%)比较, ASC组(7.72%±2.05%)略有升高, 但差异无统计学意义(P>0.05);CHB组(9.01%±2.92%)和HCC组(10.86%±1.77%)均明显升高(P<0.01), 且HCC组高于CHB组(P<0.05)。Treg细胞与ALT、AST均呈正相关[r值分别为0.452(P<0.01)、0.307(P<0.05)]。外周血Th17细胞比例与Treg细胞比例呈正相关(r=0.327, P<0.01)。正常对照组Th17/Treg最低, 为0.17±0.16, ASC组略有上升(0.18±0.13), 但差异无统计学意义(P>0.05);CHB组(0.23±0.19)和HCC组(0.52±0.54)明显升高(P<0.05、P<0.01)。结果 HBV感染后随着疾病的发生、发展, 外周血Th17细胞和Treg细胞的比例增加。由于Th17细胞和Treg细胞构成的平衡被打破, 趋向Th17细胞所介导的炎症反应, 引起肝细胞损伤。

关键词: 辅助性T细胞17, 调节性T细胞, 乙型肝炎病毒

Abstract:

Objective To investigate the percentage changes of T helper 17 (Th17) cells and regulatory T (Treg) cells in the peripheral blood of hepatitis B virus(HBV) infected patients and their correlations with liver injury after HBV infection. Methods The peripheral blood of 34 asymptomatic HBV carrier(ASC), 50 chronic hepatitis B(CHB) patients and 32 hepatocellular carcinoma(HCC) patients were collected. A total of 30 healthy controls(control group) were enrolled. The percentages of Th17 and Treg cells were detected by flow cytometry. Alanine aminotransferase (ALT) and aspartate aminotransferase(AST) were detected by Bayer ADVIA 1650 automatic biochemistry analyzer. Results Compared with control group (1.13%±0.37%), the percentage of Th17 cells in the peripheral blood of ASC group (1.30%±0.28%) was higher with no statistical significance (P> 0.05), but it obviously increased in CHB group (2.06%±1.15%) and HCC group (5.89%±3.31%, P<0.01). The percentage of Th17 cells in HCC group was higher than that in CHB group (P<0.05). The percentage of Th17 cells was positively correlated with ALT and AST [coefficients of correlation(r) were 0.682 and 0.637, P<0.01]. Compared with control group (7.18%±2.28%), the percentage of CD4⁺CD25⁺CD127^lowTreg cells in the peripheral blood of ASC group (7.72%±2.05%) was higher with statistical significance (P> 0.05), but it increased obviously in CHB group (9.01%±2.92%) and HCC group (10.86%±1.77%, P<0.01), and that in HCC group was higher than that in CHB group(P<0.05). The correlation of Treg cells was positive with ALT and AST (r=0.452, P<0.01; r=0.307, P<0.05). The correlation of Th17 cell percentage was positive with Treg cell percentage (r=0.327, P<0.01). The rate of Th17/Treg in control group was the lowest (0.17±0.16), that in ASC group(0.18±0.13) increased slightly, and there was no statistical significance between control group and ASC group (P> 0.05). However, there was obviously increasing in CHB group (0.23±0.19, P<0.05) and HCC group (0.52±0.54, P<0.01). Results In HBV infected patients, the percentages of Th17 and Treg cells increase. As for the immune balance of Th17 and Treg cells is broken, the increasing of Th17 cells mediates immune injury and induces the inflammation of liver cells.

Key words: T helper 17 cell, Regulatory T cell, Hepatitis B virus

中图分类号:

R446.63

胡月明, 马莉, 李虹. HBV感染者外周血Th17细胞和Treg细胞的检测及意义[J]. 检验医学, 2014, 29(1): 21-25.

HU Yueming, MA Li, LI Hong.. The detections of peripheral blood Th17 cells and Treg cells in HBV infected patients and their significance[J]. , 2014, 29(1): 21-25.

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HBV感染者外周血Th17细胞和Treg细胞的检测及意义

The detections of peripheral blood Th17 cells and Treg cells in HBV infected patients and their significance

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