检验医学 ›› 2015, Vol. 30 ›› Issue (6): 626-630.DOI: 10.3969/j.issn.1673-8640.2015.06.020

• 基础研究·论著 • 上一篇    下一篇

耐碳青霉烯类药物鲍曼不动杆菌的碳青霉烯酶、整合酶基因型研究

卜劲松, 王勇   

  1. 绍兴市上虞人民医院检验科,浙江 绍兴 312300
  • 收稿日期:2014-12-19 出版日期:2015-06-30 发布日期:2015-07-03
  • 作者简介:null

    作者简介:卜劲松,女,1969年生,学士,主任技师,主要从事分子生物学方面研究。

  • 基金资助:
    浙江省医药卫生科技计划资助项目(2012KYA176)

Study on carbapenemase and integrase genotypes of carbapenem-resistant Acinetobacter baumannii

BU Jingsong, WANG Yong   

  1. Department of Clinical Laboratory, Shangyu People's Hospital, Zhejiang Shaoxing 312300, China
  • Received:2014-12-19 Online:2015-06-30 Published:2015-07-03

摘要: 目的

研究碳青霉烯类耐药的鲍曼不动杆菌碳青霉烯酶、整合酶基因型情况。

方法

收集93株碳青霉烯类耐药的鲍曼不动杆菌和16株碳青霉烯类敏感的鲍曼不动杆菌,采用改良Hodge实验检测碳青霉烯酶,EDTA协同试验检测金属β-内酰胺酶;采用聚合酶链反应(PCR)扩增和测序分析碳青霉烯酶和整合子基因。

结果

耐药株碳青霉烯酶表型检测阳性率为62.4%,金属酶表型检测均为阴性;PCR结果显示耐药菌中OXA-23、Int1检出率为100%,OXA-51检出率为96.8%,OXA-58检出率为1.1%,未检出OXA-24、VIMIMPInt2,敏感株除Int1检出率为37.5%外,其余基因均未检出。

结论

临床分离的碳青霉烯类耐药的鲍曼不动杆菌主要携带OXA-23、OXA-51碳青霉烯酶基因型,并且均携带Ⅰ类整合酶基因。

关键词: 鲍曼不动杆菌, 碳青霉烯酶, 整合酶, 基因型别

Abstract: Objective

To investigate the genotypes of carbapenemase and integrase by carbapenem-resistant Acinetobacter baumannii (CRAB).

Methods

A total of 93 isolates of CRAB and 16 isolates of carbapenem-sensitive Acinetobacter baumannii (CSAB) were collected. Carbapenemases were detected by modified Hodge testing. Metallo-beta-lactamase was detected by EDTA collaborative testing. Carbapenemase and integrase genotypes were amplified by polymerase chain reaction(PCR)and sequencing.

Results

The carbapenemase phenotype test results showed that 62.4% isolates were positive, and metal enzymes were all negative. The PCR results for CRAB showed the detection rates of OXA-23 and Int1 were 100%, for OXA-51 was 96.8%, for OXA-58 was 1.1%, and OXA-24, VIM, IMP and Int2 were not found. The PCR results for CSAB showed the detection rate of Int1 was 37.5%.

Conclusions

CRAB mainly carrys OXA-23 and OXA-51 carbapenemase genes, and all isolates carry ClassⅠ integrase gene.

Key words: Acinetobacter baumannii, Carbapenemase, Integrase, Genotype

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