细菌整合子检测方法研究进展

doi:10.3969/j.issn.1673-8640.2022.09.019

摘要/Abstract

摘要：

细菌整合子具有独特的结构,能够捕获和表达外源基因,通过转座子和质粒可以在细菌间水平传播。多重耐药细菌的出现是临床抗感染治疗面临的巨大挑战,而耐药细菌多携带整合子。因此,整合子的早期检测对尽早判断细菌耐药非常重要。目前,检测细菌整合子的方法主要是基于整合子的整合酶及其基因盒。文章对基于聚合酶链反应（PCR）的各种检测方法和环介导等温扩增（LAMP）、宏基因组测序（mNGS）、基因芯片等细菌整合子检测方法,以及各种检测方法的原理和特点进行介绍,并比较每种方法的优缺点,帮助临床实验室选择更适合的检测方法,及时了解细菌携带整合子的情况,尽早确定细菌耐药特点,为临床诊治提供参考。

关键词: 细菌整合子, 基因盒, 整合酶, 耐药

Abstract:

Bacterial integrons have unique structures that can capture and express foreign genes,and they can be transmitted horizontally between bacteria through transposons and plasmids. The emergence of multi-drug-resistant bacteria is a challenge for clinical treatment,and many drug-resistant bacteria carry integrons. Therefore,the early determination of integrons is important for the early determination of bacterial drug resistance. At present,the methods of bacterial integron determination are mainly to determine the integrase of integron and to determine gene cassettes. This review introduces several bacterial integron determination methods,including the determination methods based on polymerase chain reaction（PCR）,loop-mediated isothermal amplification（LAMP）,metagenomic next generation sequencing（mNGS）,gene chip technology. The principle and characteristics of various determination methods are introduced,and the advantages and disadvantages of methods are compared. This review provides a reference for finding a better determination method for clinical use,so as to grasp the situation of bacterial integron carrying in real time and respond to bacterial drug resistance as soon as possible.

Key words: Bacterial integron, Gene cassette, Integrase, Drug resistance

中图分类号:

R446.5

郝佳慧, 杨泽华. 细菌整合子检测方法研究进展[J]. 检验医学, 2022, 37(9): 887-891.

HAO Jiahui, YANG Zehua. Research progress of bacterial integron determination methods[J]. Laboratory Medicine, 2022, 37(9): 887-891.

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