FIB基因rs6056位点单核苷酸多态性及FIB浓度与糖尿病足的相关性研究

doi:10.3969/j.issn.1673-8640.2015.04.019

摘要/Abstract

摘要：

目的探讨纤维蛋白原(FIB)基因rs6056位点单核苷酸多态性(SNP)的分布频率与糖尿病足遗传易感性的关系以及对血浆FIB浓度的影响。方法采用多重聚合酶链反应-连接酶检测反应法(PCR-LDR)检测123例糖尿病足患者(DF组)、97例糖尿病患者(T2DM组)及80名健康体检者(正常对照组)FIB基因rs6056位点的SNP,同时收集一般临床资料并检测空腹血糖(FPG)、餐后2 h血糖(2 hPG)、白细胞(WBC)计数及FIB等指标。结果DF组WBC和FIB水平明显高于T2DM组及正常对照组(P均<0.05),DF组糖尿病病程较T2DM组长,3组间年龄和性别构成差异无统计学意义(P>0.05)。DF组中Wagner Ⅴ级患者的FIB和WBC水平均明显高于Wagner Ⅳ级和Wagner 0~Ⅲ级患者。DF组TT基因型的FIB水平明显高于CC和CT基因型(P均<0.05);Wagner 0~Ⅲ级组TT和CT基因型的FIB水平均明显高于CC基因型(P均<0.05),Wagner Ⅳ级组TT基因型的FIB水平明显高于CC基因型(P<0.05),Wagner Ⅴ级组3种基因型之间FIB水平差异无统计学意义(P>0.05)。单因素Logistic回归分析显示,WBC、FIB、CT基因型、TT基因型、T等位基因为DF的危险因素。多因素Logistic回归分析显示校正WBC和FIB后,TT基因型和T等位基因不是DF发生的独立危险因素。结论TT基因型与FIB水平升高相关,FIB基因rs6056位点可能与DF的发生、发展有关。

关键词: 纤维蛋白原, 单核苷酸多态性, rs6056位点, 糖尿病足

Abstract:

Objective To explore the association between the rs6056 single nucleotide polymorphism (SNP) of fibrinogen (FIB) gene distribution rate and gentic predisposition with diabetic foot and the influence on functional expression of plasma FIB. Methods Using the polymerase chain reaction-ligation detection reaction (PCR-LDR) method, rs6056 polymorphism of FIB gene was studied in 123 patients with diabetic foot (DF group), 97 patients with type 2 diabetes mellitus (T2DM group) and 80 controls (control group). Serum samples were collected at the recruit time for the determination of fasting blood glucose (FPG), 2 hour postprandial blood glucose (2 hPG), white blood cell (WBC) count, FIB and other indicators. Results The levels of WBC and FIB in DF group were significantly higher than those in T2DM group and control group (P<0.05). The diabetic duration of DF group was longer than that of T2DM group. Age and gender had no significant difference among three groups (P>0.05). The serum FIB and WBC of Wagner Ⅴgrade group were significantly higher than those of Wagner Ⅳ grade group and Wagner 0~Ⅲ grade group (P<0.05). Serum FIB of TT genotype was significantly higher than CT and CC genotype in DF group (P<0.05). The serum levels of FIB of TT and CT genotype were significantly higher than those of CC gerotype in Wagner 0~Ⅲ grade group (P<0.05). The serum FIB of TT genotype was significantly higher than CT and CC genotype in Wagner Ⅳgrade group (P<0.05). The serum levels of FIB had no significant difference among three genotypes with WagnerⅤgrade group (P>0.05). Univariate Logistic regression analysis showed that WBC, FIB, CT genotype, TT genotype and T allele were risk factors for DF occurrence. Multivariate Logistic regression analysis showed that TT genotype and T allele were not independent risk factors of diabetic foot, after it was adjusted for WBC and FIB. Conclusions TT genotype is associated with the level of FIB. The FIB gene rs6056 polymorphism may relate to the occurrence and development of diabetic foot.

Key words: Fibrinogen, Single nucleotide polymorphism, rs6056, Diabetic foot

中图分类号:

R446.1

赵静静, 王伟灵, 周丽霞, 杨沁彤, 张莹, 赵延荣, 邢嘉翌. FIB基因rs6056位点单核苷酸多态性及FIB浓度与糖尿病足的相关性研究[J]. 检验医学, 2015, 30(4): 376-380.

ZHAO Jingjing, WANG Weiling, ZHOU Lixia, YANG Qintong, ZHANG Ying, ZHAO Yanrong, XING Jiayi. Study on correlationship of FIB concentration and rs6056 polymorphism of FIB gene with diabetic foot[J]. Laboratory Medicine, 2015, 30(4): 376-380.

图/表 8

参考文献 10

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组别	例数	年龄(岁)	病程(年)	FPG(mmol/L)	WBC(×10⁹/L)	FIB(g/L)
DF组	123	70±11	14.72±8.32^#	8.06±3.82^*	8.31±6.84^*#	3.95±1.20^*#
T2DM组	97	67±9	10.11±7.73	8.44±3.79^*	6.38±1.65	2.81±0.75^*
正常对照组	80	69±10	-	5.47±0.48	5.83±1.53	2.15±0.81

组别	例数	年龄(岁)	病程(年)	FPG(mmol/L)	WBC(×10⁹/L)	FIB(g/L)
DF组	123	70±11	14.72±8.32^#	8.06±3.82^*	8.31±6.84^*#	3.95±1.20^*#
T2DM组	97	67±9	10.11±7.73	8.44±3.79^*	6.38±1.65	2.81±0.75^*
正常对照组	80	69±10	-	5.47±0.48	5.83±1.53	2.15±0.81

Wagner分级	例数	年龄(岁)	病程(年)	BMI(kg/m²)	WBC(10⁹/L)	FIB(g/L)
0~Ⅲ级组	57	70±11	14.63±8.59	23.29±3.57	6.81±1.98	3.28±0.95
Ⅳ级组	56	70±12	14.50±8.26	23.54±4.64	8.88±9.43^*	4.33±1.08^*
Ⅴ级组	10	66±12	16.30±7.86	21.18±3.55	13.56±3.90^*#	5.63±0.36^*#

Wagner分级	例数	年龄(岁)	病程(年)	BMI(kg/m²)	WBC(10⁹/L)	FIB(g/L)
0~Ⅲ级组	57	70±11	14.63±8.59	23.29±3.57	6.81±1.98	3.28±0.95
Ⅳ级组	56	70±12	14.50±8.26	23.54±4.64	8.88±9.43^*	4.33±1.08^*
Ⅴ级组	10	66±12	16.30±7.86	21.18±3.55	13.56±3.90^*#	5.63±0.36^*#

组别	例数	基因型			等位基因
组别	例数	CC	CT	TT	C	T
DF组	123	63(51.2)	48(39.0)	12(9.8)	174(70.7)	72(29.3)
T2DM组	97	51(52.6)	36(37.1)	10(10.3)	138(71.1)	56(28.9)
正常对照组	80	60(75.0)	18(22.5)	2(2.5)	138(86.2)	22(13.8)
χ²值		13.90			14.737
P值		0.008			0.001