鄂尔多斯地区蒙古族人群耐甲氧西林金黄色葡萄球菌基因分型

doi:10.3969/j.issn.1673-8640.2014.02.005

检验医学 ›› 2014, Vol. 29 ›› Issue (2): 110-114.DOI: 10.3969/j.issn.1673-8640.2014.02.005

鄂尔多斯地区蒙古族人群耐甲氧西林金黄色葡萄球菌基因分型

王艳海, 段宝生, 巴特尔, 张静, 赵娜

内蒙古医科大学鄂尔多斯临床医学院, 内蒙古鄂尔多斯 017000

收稿日期:2013-01-09 出版日期:2014-02-20 发布日期:2014-02-20
作者简介:王艳海, 男, 1982年生, 硕士, 主管技师, 主要从事耐甲氧西林金黄色葡萄球菌的基因分型研究。
基金资助:
鄂尔多斯草原英才基金项目(20121011);内蒙古自治区自然科学基金项目(2013MS1116)

Genotype of methicillin-resistant Staphylococcus aureus from Mongolian in Ordos

WANG Yanhai, DUAN Baosheng, BA Teer, ZHANG Jing, ZHAO Na.

The Clinical Medicine College of Ordos, Inner Mongolia Medical University, Inner Mongolia Autonomous Region Ordos 017000, China

Received:2013-01-09 Online:2014-02-20 Published:2014-02-20

摘要/Abstract

摘要：

目的探讨鄂尔多斯地区蒙古族人群耐甲氧西林金黄色葡萄球菌(MRSA)的分子流行病学特点, 明确本地区MRSA基因型别及其分布规律。方法收集2009年1月至2011年8月临床分离的MRSA菌株54株。应用多重聚合酶链反应(PCR)对MRSA菌株进行葡萄球菌染色体mec(SCCmec)基因分型、杀白细胞毒素(PVL)毒力基因(pvl)和多位点序列分型(MLST)检测。结果 MRSA菌株的SCCmec Ⅰ~Ⅴ基因分型分别占0.00%、50.00%、46.30%、1.85%和1.85%;检测出1株pvl基因阳性;24株MRSA菌株MLST分型显示, ST239 13株(54.17%), ST59株(37.50%), ST5 9和ST7各1株(4.17%)。结论鄂尔多斯地区蒙古族人群MRSA菌株以SCCmec Ⅱ型和SCCmecⅢ型为主。

关键词: 耐甲氧西林金黄色葡萄球菌, 染色体mec基因盒, 多位点序列分型, 杀白细胞毒素基因

Abstract:

Objective To investigate the molecular prevalence characteristics of methicillin-resistant Staphylococcus aureus (MRSA) from Mongolian in Ordos, and to investigate the genotypes and distribution pattern of MRSA isolates. Methods A total of 54 MRSA isolates were collected from January 2009 to August 2011.The multiplex polymerase chain reaction(PCR) was used to determine Staphylococcal cassette chromosome mec (SCCmec) genotypes, Panton-Valentine leukocidin (PVL) gene(pvl) and multi-locus sequence typing (MLST) of MRSA isolates. Results The main SCCmec Ⅰ-Ⅴ genotypes of MRSA isolates, accounted for 0.00%, 50.00%, 46.30%, 1.85% and 1.85%, respectively. The pvl gene had been discovered in only 1 isolate. Among 24 MRSA isolates, MLST results showed that 13 isolates were ST239(54.17%), 9 isolates were ST5(37.50%), 1 isolate was ST59 (4.17%), and 1 isolate was ST7 (4.17%). Conclusions The main genotypes of MRSA are SCCmec Ⅱ and SCCmec Ⅲ from Mongolian in Ordos.

Key words: Methicillin-resistant Staphylococcus aureus, Staphylococcal cassette chromosome mec, Multi-locus sequence typing, Panton-Valentine leukocidin

中图分类号:

R378.1

王艳海, 段宝生, 巴特尔, 张静, 赵娜. 鄂尔多斯地区蒙古族人群耐甲氧西林金黄色葡萄球菌基因分型[J]. 检验医学, 2014, 29(2): 110-114.

WANG Yanhai, DUAN Baosheng, BA Teer, ZHANG Jing, ZHAO Na.. Genotype of methicillin-resistant Staphylococcus aureus from Mongolian in Ordos[J]. , 2014, 29(2): 110-114.

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鄂尔多斯地区蒙古族人群耐甲氧西林金黄色葡萄球菌基因分型

Genotype of methicillin-resistant Staphylococcus aureus from Mongolian in Ordos

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