Table of Content

30 March 2019, Volume 34 Issue 3

Previous Issue    Next Issue

Consensus of method development and validation of liquid chromatography-tandem mass spectrometry in clinical laboratories

Clinical Mass Spectrometry Committee, Chinese Medical Doctor Association of Laboratory Medicine

2019, 34(3):  189-196.  DOI: 10.3969/j.issn.1673-8640.2019.03.001

Asbtract ( 456 )   HTML ( 23)   PDF (837KB) ( 1397 )  

Figures and Tables | References | Related Articles | Metrics

Liquid chromatography-tandem mass spectrometry （LC-MS/MS） is an emerging technology which has developed rapidly in recent years. It has combined the separation properties of liquid chromatography and the high sensitivity and specificity of mass spectrometry,which is widely applied in various areas,such as chemistry,biology,pharmaceutical science,food,clinic and environmental science. Especially in the field of clinical laboratory and diagnostics,LC-MS/MS used as a complement to traditional diagnostic techniques can often provide more accurate and reliable testing results in accurate and rapid diagnosis of diseases. In this review,some internationally published LC-MS/MS method validation guidelines,related literature and practical experience were summarized,and some key processes of LC-MS/MS development were introduced. Using 25-hydroxyvitamin D₃[25（OH）D₃] as an example,the key elements of method validation were reviewed,in order to provide a reference for the establishment,verification and implementation of LC-MS/MS.

Interference of monoclonal immunoglobulin to immunoglobulin determination by immunoturbidimetry and nephelometry

HAN Jianhua, CHENG Xinqi, WU Jie, JI Wei, DI Qian, ZHANG Junbao, JIN Cheng, SU Wei

2019, 34(3):  197-201.  DOI: 10.3969/j.issn.1673-8640.2019.03.002

Asbtract ( 273 )   HTML ( 6)   PDF (787KB) ( 421 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the interference of monoclonal immunoglobulin（M protein） to immunoglobulin determination by immunoturbidimetry and nephelometry. Methods A total of 25 healthy subjects（healthy control group） and 54 patients with different concentrations and different types of M proteins （20 cases of IgG,16 cases of IgA and 18 cases of IgM） were enrolled. Their serum specimens were collected. IgG,IgA and IgM were determined by immunoturbidimetry and nephelometry. The interference of M protein to the other immunoglobulins was evaluated through interference and recovery experiments. Results The results of IgG,IgA and IgM determinations by nephelometry were higher than those by immunoturbidimetry （P<0.05）. The relative differences of immunoturbidimetry and nephelometry for IgG and IgM determinations in healthy control group were 4.86% and -1.00%,respectively,which were less than allowable total error（TEa）. However,the relative difference for IgA （15.42%） was higher than TEa. For IgG type and IgM type M protein patients,the IgG and IgM results' relative differences between the 2 methods were 14.38% and 4.06%,respectively,which were higher than those in healthy control group （P<0.05）. However,for IgA type M protein patients,the relative difference was 11.95%,which was lower than that in healthy control group （P<0.05）. When IgG level of IgG type M protein was ≥35.13 g/L,the IgA recovery results' difference（Diff%） for IgA was near or over TEa by immunoturbidimetry. When IgG level reached 43.64 g/L,the Diff% for IgM was lower than TEa by immunoturbidimetry. The same level of IgG type M protein did not show obvious interference effect on nephelometry. For immunoturbidimetry,IgA and IgM type M proteins interfered on IgG determination,and the Diff% was higher than IgG corresponding TEa. However,for nephelometry,the relative high levels of IgA and IgM interfered on IgG determination. For either immunoturbidimetry or nephelometry,there was interference of IgA type M protein on IgM determination or IgM type M protein on IgA determination,and their Diff% were higher than IgM and IgA corresponding TEa,respectively. Conclusions IgG,IgA and IgM type M proteins interfere on the other 2 immunoglobulins in same specimens,and the interference depends on M protein level. Nephelometry shows better anti-interference of M protein than immunoturbidimetry.

Changes of serum 1,25-dihydroxyvitamin D₃,IL-17 and IL-10 in patients with osteoarthritis

JIANG Wenjia

2019, 34(3):  202-205.  DOI: 10.3969/j.issn.1673-8640.2019.03.003

Asbtract ( 280 )   HTML ( 6)   PDF (770KB) ( 199 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the changes of serum 1,25-dihydroxyvitamin D₃ [1,25（OH）₂D₃],interleukin （IL）-17 and IL-10 in patients with osteoarthritis （OA）. Methods A total of 91 OA patients and 80 healthy subjects （healthy control group） were enrolled. The levels of 1,25（OH）₂D₃,IL-17 and IL-10 were determined by enzyme-linked immunosorbent assay （ELISA）. The level of 25-hydroxyvitamin D₃ [25（OH）D₃] was determined by electrochemiluminescence. Visual analogue scale （VAS） was used for the scoring of OA patients. The correlations among 1,25（OH）₂D₃,VAS,IL-17 and IL-10 were analyzed by Spearman correlation analysis. Results Serum levels of 1,25（OH）₂D₃,25（OH）D₃ and IL-10 were lower in OA group than those in healthy control group （P<0.05）,and serum level of IL-17 in OA group was higher （P<0.05）. According to the levels of 25（OH）D₃,the patients were classified into 3 groups,vitamin D deficiency,vitamin D insufficient and vitamin D adequacy groups. The VAS score of vitamin D deficiency group was higher than those of vitamin D insufficient and vitamin D adequacy groups （P<0.05）,and there was no statistical significance for the VAS score between vitamin D insufficient and vitamin D adequacy groups （P>0.05）. According to VAS scores,the patients were classified into mild pain,moderate pain and severe pain groups. Serum levels of 1,25（OH）₂D₃ in severe pain and moderate pain groups were lower than that in mild pain group （P<0.05）,and there was no statistical significance between severe pain and moderate pain groups （P>0.05）. Spearman correlation analysis showed that 1,25（OH）₂D₃ was positively correlated with IL-10 （r=0.778,P=0.002）,and it was negatively correlated with VAS and IL-17 （r=-0.691 and -0.735,P=0.01 and 0.007）. VAS score was negatively correlated with IL-10 （r=-0.725,P=0.008） and positively correlated with IL-17 （r=0.699,P=0.010）. Serum levels of 1,25（OH）₂D₃ and IL-10 in patients with OA after treatment were higher than those before treatment （P<0.05）,but they were lower than those in healthy control group （P<0.05）. Serum levels of IL-17 after treatment were lower than those before treatment （P<0.05）,but they were higher than those in healthy control group （P<0.05）. Conclusions Serum 1,25（OH）₂D₃ plays a role in the occurrence and severity of OA.

Expression of metastasis associated protein-3 in breast cancer tissue and its relationship with axillary lymph node metastasis

XIONG Yumin, WANG Zhiwei, JIN Xin, HONG Wei, LI Fuguang, YAO Shupeng, ZHAO Zilong, LIU Jun, GAO Xiaopeng

2019, 34(3):  206-210.  DOI: 10.3969/j.issn.1673-8640.2019.03.004

Asbtract ( 204 )   HTML ( 8)   PDF (1229KB) ( 260 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the expression of metastasis associated protein （MTA）-3 in breast cancer patients and its relationship with axillary lymph node metastasis. Methods The tumor tissues of 122 patients with breast cancer （infiltrating ductal carcinoma）and normal breast tissues （control group） of 20 cyclomastopathy patients were collected. Immunohistochemistry was used for determining the expression of MTA-3. Combined with their clinicopathological data,the relations of MTA-3 with clininopathological characteristics,Ki-67 and epithelial cadherin（E-cadherin） were evaluated. According to the status of axillary lymph node metastasis,the patients with breast cancer were classified into metastasis group （55 cases） and non-metastasis group （67 cases）. Results The expression of MTA-3 in breast cancer group （32.8%） was lower than that in control group（85.0%）（P=0.000）. MTA-3 expression was related with histological differentiation,clinical stage,primary tumor size,axillary lymph node metastasis and molecular classification （P<0.05）,without age,menstruation and body mass index （P>0.05）. There was statistical significance in histological differentiation,clinical stage and molecular classification between metastasis and non-metastasis groups（P<0.05）. There was no statistical significance in age,menstruation,body mass index or primary tumor size（P>0.05）. Multivariate Logistic regression analysis showed that MTA-3 expression was a protective factor of axillary lymph node metastasis for patients with breast cancer [odds ratio （OR）=0.662,95% confidence interval （CI） 0.246-0.891]. The expression of MTA-3 had negative correlations with the number of axillary lymph node metastasis（r=-0.197,P=0.030） and Ki-67（r=-0.358,P=0.000）,while there was a positive correlation with E-cadherin （r=0.237,P=0.009）. Conclusions MTA-3 is lowly expressed and is related with axillary lymph node metastasis in breast cancer.

Influence of atorvastatin calcium tablets on small and dense low-density lipoprotein cholesterol in myocardial infarction patients

PAN Yue, HE Yong, JIN Jing, WANG Rongqi

2019, 34(3):  211-214.  DOI: 10.3969/j.issn.1673-8640.2019.03.05

Asbtract ( 166 )   HTML ( 6)   PDF (782KB) ( 264 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the change of small and dense low-density lipoprotein cholesterol （sd-LDL-C） in myocardial infarction patients and the level change of sd-LDL-C after taking atorvastatin calcium tablets. Methods The levels of total cholesterol （TC）,triglyceride （TG）,high-density lipoprotein cholesterol （HDL-C）,low-density lipoprotein cholesterol （LDL-C） and sd-LDL-C in 96 patients with myocardial infarction before treatment and after treatment with atorvastatin calcium tablets orally for 4 weeks and 50 healthy subjects （healthy control group） were determined. Coronary computed tomography （CT） was used to determine the degree of coronary stenosis. The patients with myocardial infarction were classified into 3 groups according to the coronary CT results （<50%,50%-70% and >70%）. Results Compared to healthy control group,blood pressure,TG,LDL-C,sd-LDL-C and sd-LDL-C/LDL-C ratio in myocardial infarction group were increased （P<0.05）,and HDL-C was decreased （P<0.05）. There was no statistical significance for TC between the 2 groups （P>0.05）. TC,sd-LDL-C and sd-LDL-C/LDL-C ratios were increased in turn in coronary stenosis <50%,50%-70% and >70% groups （P<0.05,P<0.01）,and other indicators had no statistical significance （P>0.05）. Logistic regression analysis showed that TC,sd-LDL-C and sd-LDL-C/LDL-C ratio were risk factors for coronary stenosis>70% [odds ratios （OR） were 2.680,2.000 and 7.819,95% confidence intervals （CI） were 1.780-7.030,1.103-3.681 and 1.430-40.263]. In coronary stenosis>70% group,sd-LDL-C and sd-LDL-C/LDL-C ratio after treatment were lower than those before treatment （P<0.05）,and there was no statistical significance for coronary stenosis <50% and 50%-70% groups （P>0.05）. Conclusions Sd-LDL-C and sd-LDL-C/LDL-C ratio can be used as risk factors for myocardial infarction. The atorvastatin calcium tablets can decrease sd-LDL-C and sd-LDL-C-c/LDL-C ratio effectively.

Determination and drug resistance gene of methicillin-resistant Staphylococcus aureus isolated from adults in a hospital of Nanjing

ZHANG Li, SUN Linchun

2019, 34(3):  215-220.  DOI: 10.3969/j.issn.1673-8640.2019.03.006

Asbtract ( 238 )   HTML ( 7)   PDF (1255KB) ( 413 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the determination and drug resistance gene of methicillin-resistant Staphylococcus aureus （MRSA） isolated from adults in Jiangsu Province Hospital on Integration of Chinese and Western Medicine from 2011 to 2017,and to provide a reference for clinical rational drug use. Methods According to the Clinical and Laboratory Standards Institute （CLSI） guidelines,MRSA were identified. The drug susceptibility was determined by disk diffusion method. The β-lactam resistance gene mecA,aminoglycoside modifying enzyme genes aac（6′）/aph（2″）and aph（3）-Ⅲ,macrolide 23s rRNA methylase gene ermA/C,tetracycline-like ribosomal protective protein gene tetM/K,quinolone resistance gene qnrA/B and disinfectant resistance gene qacA/B were determined by polymerase chain reaction （PCR）. Results The determination rate of MRSA was 31.95%,and the isolates of MRSA were mainly isolated from sputum and wound secretion samples. The top 3 departments were respiratory department,surgery department and intensive care unit. There was no MRSA isolate being resistant to vancomycin,linezolid and tigecycline. There were 0.49% MRSA isolates being resistant to quinupristin. The drug resistance rates to cotrimoxazole,rifampicin,ciprofloxacin,tetracycline,gentamicin,clindamycin and erythromycin were 11.18%,11.57%,21.47%,63.63%,44.31%,59.41% and 62.35%,respectively. The determination rate of mecA was 100%. The determination rates of aac（6′）/aph（2″）,aph（3）-Ⅲ,ermA/C,tetM/K,qnrA/B and qacA/B were 36.67 %,27.94%,68.43%,57.35%,23.63% and 52.45%,respectively.Conclusions From 2011 to 2017,the determination rate of MRAS multidrug resistance gene is high. MRSA shows various degrees of drug resistance to many antibiotics. Drug susceptibility test's results can be used reference for clinical medication.

Different methods for the rapid diagnosis of tuberculous pleurisy

SHEN Jie, JIA Li, DONG Xiaoshuai, MENG Cheng

2019, 34(3):  221-224.  DOI: 10.3969/j.issn.1673-8640.2019.03.007

Asbtract ( 209 )   HTML ( 6)   PDF (789KB) ( 359 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the roles of different methods for the rapid diagnosis of tuberculous pleurisy,and to find a method for rapid diagnosis. Methods A total of 50 patients with undiagnosed exudative pleural effusion were enrolled. Using thoracoscopy,the biopsy specimens of parietal pleura were taken under direct vision. Pleural effusion and biopsy specimens were subjected to bacterial culturing,polymerase chain reaction （PCR）,histopathological microscopic examination （directly and after cytocentrifugation）. Mononuclear cells were isolated from pleural effusion and determined by T cell spot test. Results The biopsy specimens proved to have a higher determination rate of Mycobacterium tuberculosis than pleural effusion. Cytocentrifugation improved the sensitivity of microscopic examination for pleural effusion increasing by 20.0% and biopsy specimens increasing by 37.5%. T cell spot test had higher sensitivity （98.6%） and specificity （95.6%）. Conclusions The combination of microbiological examination and histopathological examination of biopsy specimens is the best method for the diagnosis of tuberculous pleurisy,and microbiological examination using biopsy specimens has a higher determination rate than using pleural effusion. T cell spot test is worth the clinical application for the diagnosis of tuberculous pleurisy.

Serum PCT in the treatment of bloodstream infection in children

TIAN Dongxing, PAN Fen, SHI Yingying, JIANG Linlin, GAO Yuan, ZHANG Hong

2019, 34(3):  225-228.  DOI: 10.3969/j.issn.1673-8640.2019.03.008

Asbtract ( 181 )   HTML ( 4)   PDF (783KB) ( 300 )  

Figures and Tables | References | Related Articles | Metrics

Objective To analyze the role of serum procalcitonin （PCT） in the treatment of bloodstream infection in children. Methods Serum PCT of 79 children with bloodstream infection was determined on the 1st,4th and 7th days,respectively,and the clinical data were also collected. Results A total of 37 Gram-positive bacteria and 42 Gram-negative bacteria were isolated. The 1st day PCT of Gram-positive bacteria and 42 Gram-negative bacteria were 7.56（5.33-13.49） ng/mL and 8.96（5.36-21.34） ng/mL,respectively （P>0.05）. On the 4th day,PCT in the 2 groups decreased to 1.10（0.42-3.62） ng/mL and 3.41（1.54-9.95） ng/mL,respectively （P<0.05）. On the 7th day,PCT decreased to 0.13（0.10-0.34） ng/mL and 0.23（0.12-1.92） ng/mL,respectively（P<0.05）. The decreasing rates of PCT differed among different pathogens. Serum PCT of Streptococcus pneumoniae decreased fastly,with 98.57% decreasing on the 4th day and 99.21% decreasing on the 7th day,that of Acinetobacter baumannii decreased slowly,with 33.15% and 46.30% decreasing,respectively. The change of PCT was consistent with the prognosis of children. The antibiotic usage time was 17（11.00-22.50） d. The costs of antibiotics for Gram-positive bacteria and Gram-negative bacteria were 206.34（114.00-389.61） Yuan/d and 297.29（224.62-502.60） Yuan/d,respectively. Conclusions PCT can be used to evaluate the treatment efficiency of antibiotic therapy for bloodstream infection,to adjust the antibiotic usage time and antibiotic therapy timely,which can reduce drug resistance and adverse reactions.

Clinical characteristics for 11 cases of TA-TMA after HSCT

WANG Yuanyuan, FU Shuzhen

2019, 34(3):  229-234.  DOI: 10.3969/j.issn.1673-8640.2019.03.009

Asbtract ( 191 )   HTML ( 5)   PDF (889KB) ( 397 )  

Figures and Tables | References | Related Articles | Metrics

Objective To analyze the clinical characteristics of transplantation-associated thrombotic microangiopathy （TA-TMA） after hematopoietic stem cell transplantation （HSCT）. Methods A total of 185 patients undergoing HSCT were enrolled. Totally,11 cases were confirmed as TA-TMA （case group）,and 30 non-TA-TMA cases with similar age,transplantation ways and human leukocyte antigen matching donor were enrolled as control group. The ratios of broken red blood cells in peripheral blood between the 2 groups were compared,and the laboratory indicators and clinical characteristics of 11 cases of TA-TMA were analyzed. Results Of the 185 patients undergoing HSCT,11 cases （5.9%,11/185） were TA-TMA. The age was 33（7-45） years old,and the onset time after transplantation was 76（22-152） d. The ratio of broken red blood cells in case group was 3.3%,which was higher than that in control group （0.4%）（P<0.05）. In the 11 cases of TA-TMA,hemoglobin （Hb） level and platelet （PLT）count decreased progressively,and lactate dehydrogenase （LDH）,serum creatinine（Cr）,serum total bilirubin（TB）,C-reactive protein（CRP）,procalcitonin （PCT） and cyclosporin A （CsA） were at high levels. There were 8 cases with acute and chronic graft-versus-host diseases（GVHD） and 6 cases with positive 24 h urinary protein. Except 1 case was not detected for virus DNA,and the other cases were associated with more than 1 type of virus disease. Only 4 cases were alive until the end of follow-up period. Conclusions TA-TMA is an extremely serious complication after bone marrow transplantation. The prognosis is poor,when GVHD is combined. The determinations of a number of early laboratory indicators are necessary for the early diagnosis and treatment of TA-TMA to decrease mortality.

Bone marrow cell morphology and immuno-phenotype characteristics of MPAL by flow cytometry

WANG Xia, ZHANG Bin

2019, 34(3):  235-239.  DOI: 10.3969/j.issn.1673-8640.2019.03.010

Asbtract ( 196 )   HTML ( 4)   PDF (1797KB) ( 191 )  

Figures and Tables | References | Related Articles | Metrics

Objective To evaluate the bone marrow cell morphology and immuno-phenotype characteristics of mixed phenotype acute leukemia （MPAL）patients by flow cytometry,and to provide a reference for the diagnosis of leukemia. Methods The clinical data of 9 MPAL patients were collected,and their bone marrow cell morphology and immuno-phenotype characteristics were evaluated. Results The CD34 positive expression rate of 9 MPAL patients was 77.78%. The CD34 positive expression rates of acute myeloid and lymphoid monophenotypic leukemias were 51.74% and 60.00%,respectively. The immuno-phenotypes were mainly expressed in cMPO,cCD79a and CD3. Conclusions The characteristics of MPAL are complex. The results of bone marrow cell morphology and immuno-phenotype by flow cytometry should be analyzed combinedly in order to improve the diagnostic accuracy of MPAL.

Analysis of prenatal gene determination results in 91 couples with same type thalassemia

YU Xia, SHI Mingfang, WANG Shuangjie, ZHOU Yuanyuan, LIANG Xiuyun

2019, 34(3):  240-243.  DOI: 10.3969/j.issn.1673-8640.2019.03.011

Asbtract ( 187 )   HTML ( 4)   PDF (793KB) ( 607 )  

Figures and Tables | References | Related Articles | Metrics

Objective To analyze the results of prenatal gene determination retrospectively in 91 couples with same type thalassemia,and to provide effective genetic counseling. Methods From January 2016 to December 2017,pregnant women and their husbands receiving routine prenatal examination or genetic counseling for eugenics in Nanning Second People's Hospital were enrolled. Totally,74 couples with α-thalassemia,13 couples with β-thalassemia and 4 couples with α- and β-double thalassemia were confirmed. Genomic DNA was extracted from amniotic fluid. Gap polymerase chain reaction （Gap-PCR） and polymerase chain reaction-reverse blot hybridization assay （PCR-RDB） were used to determine the missing and mutation of thalassemia,meanwhile the karyotype was analyzed. Results In 74 couples of α-thalassemia,the main gene types were --^SEA/αα（36.5%）,--^SEA/-- ^SEA（14.9%）,-α^3.7/αα（9.5%） and α^WS α/αα（6.8%）. In 13 couples of β-thalassemia,heterozygous mutation accounted for 61.5%,double heterozygous mutation accounted for 23.1%,and homozygous mutation accounted for 7.7%。There were 4 couples of α- and β-double thalassemia,and α- and β-double thalassemia was also found in their fetuses. There were 15 fetuses of severe thalassemia,and they were all aborted. Conclusions Thalassemia screening before pregnancy and prenatal thalassemia gene determination are effective means on reducing the birth of severe thalassemia in high-risk thalassemia region.

Clinical application of latex immunoturbidimetric assay in the determination of serum Helicobacter pylori antibody

CHEN Wenju, GAO Lin, GU Wanhong, SHEN Bo

2019, 34(3):  244-245.  DOI: 10.3969/j.issn.1673-8640.2019.03.012

Asbtract ( 279 )   HTML ( 3)   PDF (793KB) ( 361 )  

Figures and Tables | References | Related Articles | Metrics

Objective To evaluate the role of latex immunoturbidimetric assay in the determination of serum Helicobacter pylori （HP） antibody. Methods A total of 120 patients with suspected HP infection who underwent gastroscopy and healthy subjects were enrolled,and Hp was determined by silver staining,latex immunoturbidimetric assay and colloidal gold rapid assay. Results Using silver staining as gold standard,the sensitivity,specificity,negative predictive value and positive predictive value of latex immunoturbidimetric assay were 93.3%,96.7%,93.6%,96.6% and 95.0%,respectively. The sensitivity and diagnostic consistency rate were higher than those of colloidal gold rapid assay（P<0.01）. Conclusions Latex immunoturbidimetric assay is simple, rapid,sensitive,specific,reproducible,accurate for the determination of serum HP antibody,especially for large-scale screening of susceptible population.

Results' analysis of human papillomavirus determination of 9 810 patients in Liuzhou,Guangxi

CAI Pengfei, XU Zehui, WANG Jingren, YAN Tizhen

2019, 34(3):  246-248.  DOI: 10.3969/j.issn.1673-8640.2019.03.013

Asbtract ( 173 )   HTML ( 3)   PDF (777KB) ( 266 )  

Figures and Tables | References | Related Articles | Metrics

Objective To analyze the results of human papillomavirus （HPV） determination in Liuzhou,Guangxi,and to provide a reference for the prevention and control of HPV infection and HPV vaccine. Methods DNA was extracted. The types of HPV were analyzed by polymerase chain reaction（PCR）. Results The total positive rate was 14.23% （1 396/9 810）. The positive rates of high-risk HPV subtypes and low-risk HPV subtypes were 10.20%（1 001/9 810） and 4.03%（395/9 810）,respectively. Among different age groups,the highest HPV carrying rate was in <20-year-old group （59.46%）. The HPV carrying rate in >60-year-old group was the lowest （0.00%）. There were 1 202 cases carrying 1 type of HPV and 147 cases carrying 2 or more types of HPV in 1 396 positive samples. In mixed infection,double infection was the majority. Conclusions HPV infection has difference in different ages and types. It should select appropriate HPV vaccine in local region.

Correlation between TSLP gene polymorphism and toddler atopic dermatitis

TANG Lin, ZHU Chenchen, XIAO Ye, WEI Bing, CAI Mei, ZHANG Xiaojing, TANG Zhenhua

2019, 34(3):  249-252.  DOI: 10.3969/j.issn.1673-8640.2019.03.014

Asbtract ( 194 )   HTML ( 2)   PDF (811KB) ( 395 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the correlation between thymic stromal lymphopoietin （TSLP）gene polymorphism in cord blood and toddler atopic dermatitis （AD）. Methods A total of 393 pregnant women in a prospective birth cohort were enrolled. Genomic DNA was extracted from cord blood samples,and polymerase chain reaction （PCR）- ligase detection reaction（LDR） was used to detect rs3806933,rs1545169,rs764917,rs1837253,rs2289276 and rs11466749 mutations in TSLP gene. Totally,285 toddlers received a phone call questionnaire at the age of 2 to determine whether they had AD or not,which was judged by pediatricians after birth, and 162 cases were diagnosed AD. Results There was no statistical significance in the genotype distribution frequencies of rs3806933,rs1545169,rs764917,rs1837253 and rs2289276 between AD group and non-AD group （P>0.05）. There was statistical significance in the genotype distribution frequency of rs11466749 （AA/AG）（AD group：rs11466749 locus 60.0% AA and 34.3% AG;non-AD group：rs11466749 locus 40.0% AA and 65.7% AG. Conclusions The gene polymorphism of TSLP gene rs11466749 in cord blood is correlated with AD.

Comparison of the results' consistency of 3 automated coagulation analyzers for coagulation items

WANG Yue, QU Chenxue, GONG Yan, GAO Xueshuo, WANG Guohong, XU Guobin

2019, 34(3):  253-258.  DOI: 10.3969/j.issn.1673-8640.2019.03.015

Asbtract ( 153 )   HTML ( 4)   PDF (947KB) ( 560 )  

Figures and Tables | References | Related Articles | Metrics

Objective To investigate the consistency of 3 automated coagulation analyzers for coagulation items [prothrombin time（PT）,international normalized ratio（INR）,activated partial thromboplastin time（APTT）,fibrinogen（Fib）,thrombin time（TT）,D-dimer（DD）and fibrin/fibrinogen degradation product（FDP）] and the matrix effects of quality control materials. Methods A total of 100 samples were collected. Totally,PT,PT-INR,APTT,Fib,TT,DD and FDP were determined by 3 automated coagulation analyzers,including CA7000,Top700 and CP2000. Linear regression analysis was used to analyze the correlation of results. The relative deviation between any 2 analyzers was calculated. The matrix effect of 18 quality control materials for PT,Fib and DD was assessed with regression. Results There were good correlations among the 3 automated coagulation analyzers for PT,PT-INR and Fib （r²=0.91-0.99）. The deviations between any 2 analyzers were <15%,15% and 20%. The results of DD among the 3 automated coagulation analyzers had correlations （r²≥0.90）,CA7000 and CP2000 had good consistency,but the results of Top700 were lower than those of CA7000 and CP2000. Moderate correlations were observed for APTT and TT（r²=0.56-0.76）. Medical kits for FDP and matched calibrators were adopted to the 3 analyzers. There was a high correlation for FDP and DD （r²>0.99,r²=0.92-0.99）,and there was difference between any 2 analyzers. There was no compatibility for PT,Fib and DD on the 3 analyzers in 18 quality control materials. Conclusions The consistency of the results of the 3 automated coagulation analyzers is diverse. The matrix effects should be considered,which makes the deviation of quality control materials greater than that of patient.

Establishment of fluorescence quantitation PCR to amplify human adenovirus-B55

DONG Lei, LIU Juan, AI Xianyin, MA Hongyu, QUAN Shouzhen, JIANG Tao

2019, 34(3):  259-262.  DOI: 10.3969/j.issn.1673-8640.2019.03.016

Asbtract ( 241 )   HTML ( 5)   PDF (878KB) ( 351 )  

Figures and Tables | References | Related Articles | Metrics

Objective To establish real-time fluorescence quantitation polymerase chain reaction （PCR）to amplify human adenovirus（HAdV）-B55,and to verify its performance. Methods The specific primers for the Hexon sequence of HAdV-B55 were designed with Beacon Designer 7 software,and the real-time fluorescence quantitation PCR was established. The sensitivity and specificity were evaluated. Results The specific primers for HAdV-B55 were obtained,and the real-time fluorescence quantitation PCR for HAdV-B55 had been established. The sensitivity was 0.08 PFU/reaction,and the specificity was good. There was no cross reaction with HAdVB Conclusions The real-time fluorescence quantitation PCR to detect HAdV-B55 has been established,which lays the foundation for the rapid detection and prevention of HAdV-B55.

Performance evaluation of GN13 and NM38 to determine the drug susceptibility of carbapenems against Klebsiella pneumoniae

ZHANG Baohua, YU Guixiang, JIANG Yan, PAN Hong, JIANG Xiangning

2019, 34(3):  263-266.  DOI: 10.3969/j.issn.1673-8640.2019.03.017

Asbtract ( 152 )   HTML ( 5)   PDF (813KB) ( 371 )  

Figures and Tables | References | Related Articles | Metrics

Objective To evaluate the reliabilities of VITEK 2-Compact GN13 and MicroScan WalkAway 96 Plus NM38 to determine the drug susceptibility of carbapenems against Klebsiella pneumoniae. Methods GN13,NM38 and broth micro-dilution method were used to determine the drug susceptibility of carbapenems to 168 isolates of Klebsiella pneumoniae which were isolated from Huangshan People's Hospital from January 2016 to August 2017. The determination results of GN13 and NM38 were judged by broth micro-dilution method as gold standard. A modified carbapenem inactivation method （mCIM） was used to determine carbapenems and to analyze their reliabilities of advanced expert system（AES） as a predictor or early warning indicator for carbapenem production. Results Using broth micro-dilution method as gold standard,GN13 and NM38 classification categorical agreement （CA） was >90%. Minor errors （MIE） and major errors （ME） for imipenem were reduced by 0.6%,and MIE for ertapenem was increased by 0.6% without very major errors （VME） after AES modification. The MIE,ME and VME determined by NM38 for imipenem accounted for 1.19%,1.19% and 2.97%,and MIE,ME and VME for ertapenem accounted for 3.6%,0.0% and 0.6%. The mCIM phenotype determination showed that the positive rate of carbapenems was 27.4% （46/168）,and the positive rates of carbapenems after GN13 AES modification and NM38 prediction were 31.0% and 30.4%,respectively. Compared with mCIM,there was no statistical significance （P>0.05）. Conclusions The results of GN13 and NM38 are reliable in determining the drug susceptibility of carbapenems against Klebsiella pneumoniae. However,attention should be paid to the modification or early warning of AES.

External quality assessment for the results of hereditary deafness genetic testing in Shanghai

BAO Yun, XIAO Yanqun, JIANG Lingli, WANG Xueliang, YANG Yixiao, WANG Hualiang

2019, 34(3):  267-270.  DOI: 10.3969/j.issn.1673-8640.2019.03.018

Asbtract ( 243 )   HTML ( 9)   PDF (722KB) ( 507 )  

Figures and Tables | References | Related Articles | Metrics

Objective To evaluate the performance of hereditary deafness genetic testing in external quality assessment （EQA） program of Shanghai. Methods In 2017 EQA program, 9 loci on 4 different genes were investigated. The sample panel consisted of 5 different dry blood spots. Participating laboratories were asked to report the results before deadlines. The scores of laboratories were calculated, and the overall consistency rates of different loci as well as different methods and reagents were calculated, and error sorting was evaluated. Results Totally, 18 valid laboratory results were received,and 77.78% laboratories submitted correct results for all samples, 5.56% laboratories reported with errors but were overall qualified, and 16.67% laboratories reported with errors and were overall unqualified. The consistency rate was 100% for negative samples （wild type samples） and 93.59% for positive samples （mutant samples）. There were 10 error results,all of which were false negative. Conclusions The overall accuracy rate of clinical laboratories enrolled in the program of Shanghai is high. Quality controls in clinical laboratories is essential to assure the accuracy of results.

Research progress of gene polymorphisms of ADH1B and ALDH2 and their related diseases

ZHANG Xiaomin, LIU Jing, GAO Shichao, YANG Tingting, WANG Peichang

2019, 34(3):  271-275.  DOI: 10.3969/j.issn.1673-8640.2019.03.019

Asbtract ( 517 )   HTML ( 29)   PDF (807KB) ( 959 )  

Figures and Tables | References | Related Articles | Metrics

Alcohol dehydrogenase （ADH） and aldehyde dehydrogenase （ALDH）are key enzymes of alcohol metabolism in human liver,which constitute ethanol dehydrogenase oxidation system. ADH1B and ALDH2 are 2 important subtypes of ADH and ALDH,respectively. ADH1B and ALDH2 gene polymorphisms are associated with a variety of diseases,such as digestive system diseases,neurodegenerative disorders and metabolic diseases. In this review,the gene polymorphisms of ADH1B and ALDH2 and their related diseases are reviewed to provide new ideas for the prevention and diagnosis of diseases.

Role of gut microbiota in gestational obesity and diabetes mellitus

ZHANG Kainan, ZHANG Zhaoxia

2019, 34(3):  276-279.  DOI: 10.3969/j.issn.1673-8640.2019.03.020

Asbtract ( 252 )   HTML ( 8)   PDF (1130KB) ( 385 )  

Figures and Tables | References | Related Articles | Metrics

Gut microbiota is a general term for all microorganisms and their genes that are colonized in human intestine. In recent years,studies have found that the changes in gut microbiota are related to the development of gestational obesity and diabetes mellitus. The changes in gut microbiota may affect the occurrence and development of related diseases by affecting certain metabolic mechanisms. However,the specific molecular biological mechanisms have not been thoroughly studied. Therefore,this review focuses on the role of gut microbiota in gestational obesity and diabetes mellitus.