Changes of serum 1,25-dihydroxyvitamin D3,IL-17 and IL-10 in patients with osteoarthritis

doi:10.3969/j.issn.1673-8640.2019.03.003

Abstract

Abstract:

Objective To investigate the changes of serum 1,25-dihydroxyvitamin D₃ [1,25（OH）₂D₃],interleukin （IL）-17 and IL-10 in patients with osteoarthritis （OA）. Methods A total of 91 OA patients and 80 healthy subjects （healthy control group） were enrolled. The levels of 1,25（OH）₂D₃,IL-17 and IL-10 were determined by enzyme-linked immunosorbent assay （ELISA）. The level of 25-hydroxyvitamin D₃ [25（OH）D₃] was determined by electrochemiluminescence. Visual analogue scale （VAS） was used for the scoring of OA patients. The correlations among 1,25（OH）₂D₃,VAS,IL-17 and IL-10 were analyzed by Spearman correlation analysis. Results Serum levels of 1,25（OH）₂D₃,25（OH）D₃ and IL-10 were lower in OA group than those in healthy control group （P<0.05）,and serum level of IL-17 in OA group was higher （P<0.05）. According to the levels of 25（OH）D₃,the patients were classified into 3 groups,vitamin D deficiency,vitamin D insufficient and vitamin D adequacy groups. The VAS score of vitamin D deficiency group was higher than those of vitamin D insufficient and vitamin D adequacy groups （P<0.05）,and there was no statistical significance for the VAS score between vitamin D insufficient and vitamin D adequacy groups （P>0.05）. According to VAS scores,the patients were classified into mild pain,moderate pain and severe pain groups. Serum levels of 1,25（OH）₂D₃ in severe pain and moderate pain groups were lower than that in mild pain group （P<0.05）,and there was no statistical significance between severe pain and moderate pain groups （P>0.05）. Spearman correlation analysis showed that 1,25（OH）₂D₃ was positively correlated with IL-10 （r=0.778,P=0.002）,and it was negatively correlated with VAS and IL-17 （r=-0.691 and -0.735,P=0.01 and 0.007）. VAS score was negatively correlated with IL-10 （r=-0.725,P=0.008） and positively correlated with IL-17 （r=0.699,P=0.010）. Serum levels of 1,25（OH）₂D₃ and IL-10 in patients with OA after treatment were higher than those before treatment （P<0.05）,but they were lower than those in healthy control group （P<0.05）. Serum levels of IL-17 after treatment were lower than those before treatment （P<0.05）,but they were higher than those in healthy control group （P<0.05）. Conclusions Serum 1,25（OH）₂D₃ plays a role in the occurrence and severity of OA.

Key words: 1,25-Dihydroxyvitamin D₃, Interleukin 17, Interleukin 10, Osteoarthritis

CLC Number:

R446.1

JIANG Wenjia. Changes of serum 1,25-dihydroxyvitamin D₃,IL-17 and IL-10 in patients with osteoarthritis[J]. Laboratory Medicine, 2019, 34(3): 202-205.

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References 18

[1]	GLYN-JONES S, PALMER A J, AGRICOLA R, et al.Osteoarthritis[J]. Lancet, 2015, 386(9991):376-387.
[2]	XIA B,DI CHEN,ZHANG J,et al.Osteoarthritis pathogenesis: a review of molecular mechanisms[J]. Calcif Tissue Int,2014,95(6):495-505.
[3]	PAPALIA R,VADALÀ G,TORRE G,et al.The cytokinome in osteoarthritis,a new paradigm in diagnosis and prognosis of cartilage disease[J]. J Biol Regul Homeost Agents,2016,30(4 Suppl 1):77-83.
[4]	LIN Z,LI W.The roles of vitamin D and its analogs in inflammatory diseases[J]. Curr Top Med Chem,2016,16(11):1242-1261.
[5]	中华医学会风湿病学分会. 骨关节炎诊断及治疗指南[J]. 中华风湿病学杂志,2010,14(6):416-419.
[6]	周白玉,廖千姿,王兴敏. 体检检测338人血清1,25-二羟基维生素D水平的分析[J]. 贵州医药,2015,39(8):740-741.
[7]	周韵娇,张丽红,李鹏,等. IL-17和 IL-23在成人原发性肾病综合征患者中的变化及意义[J]. 检验医学,2015,30(6):554-558.
[8]	杨婷婷,王青,李志,等. 银屑病患者Th17和Treg细胞及其相关细胞因子的表达[J]. 检验医学,2015,30(7):715-719.
[9]	LIU Y,PENG H,MENG Z,et al.Correlation of IL-17 level in synovia and severity of knee osteoarthritis[J]. Med Sci Monit,2015,21:1732-1736.
[10]	LI S,WAN J,ANDERSON W,et al.Downregulation of IL-10 secretion by Treg cells in osteoarthritis is associated with a reduction in Tim-3 expression[J]. Biomed Pharmacother,2016 ,79:159-165.
[11]	潘建科,郭达,刘军,等. 白细胞介素-10与骨关节炎相关性的研究进展[J]. 广东医学,2016,37(1):146-148.
[12]	吴爱成,王礼,唐恒锋,等. 2型糖尿病患者25-羟基维生素D与叶酸、同型半胱氨酸的相关性[J]. 检验医学,2017,32(10):849-852.
[13]	CRESCIOLI C,MINISOLA S.Vitamin D:autoimmunity and gender[J]. Curr Med Chem,2017,24(24):2671-2686.
[14]	JEFFERY L E,BURKE F,MURA M,et al.1,25-Dihydroxyvitamin D3,and IL-2 combine to inhibit T cell production of inflammatory cytokines and promote development of regulatory T cells expressing CTLA-4 and FoxP3[J]. J Immunol,2009,183(9):5458-5467.
[15]	王文志,徐健,王露,等. 盐酸氨基葡萄糖联合钙、维生素D治疗膝骨关节炎的疗效观察[J]. 华西医学,2016,31(7):1178-1180.
[16]	KOECKHOVEN E,VAN DER LEEDEN M,ROORDA L D,et al. The association between serum 25-hydroxy vitamin D level and upper leg strength in patients with knee osteoarthritis: results of the amsterdam osteoarthritis cohort[J]. J Rheumatol,2016,43(7):1400-1405.
[17]	BRUCE D,YU S,OOI J H,et al.Converging pathways lead to overproduction of IL-17 in the absence of vitamin D signaling[J]. Int Immunol,2011,23(8):519-528.
[18]	CHAMBERS E S,SUWANNASAEN D,MANN E H,et al.1α,25-Dihydroxyvitamin D3 in combination with transforming growth factor-β increases the frequency of Foxp3+ regulatory T cells through preferential expansion and usage of interleukin-2[J]. Immunology,2014,143(1):52-60.

组别	例数	1,25（OH）₂D₃（ng/mL）	25（OH）D₃（ng/mL）	IL-10 （pg/mL）	IL-17（pg/mL）
OA组	91	18.23±8.25^*	24.02±9.53^*	19.51±4.07^*	122.40±38.51^*
正常对照组	80	36.58±5.72	34.95±7.95	37.49±9.21	59.33±17.85

组别	例数	1,25（OH）₂D₃（ng/mL）	25（OH）D₃（ng/mL）	IL-10 （pg/mL）	IL-17（pg/mL）
OA组	91	18.23±8.25^*	24.02±9.53^*	19.51±4.07^*	122.40±38.51^*
正常对照组	80	36.58±5.72	34.95±7.95	37.49±9.21	59.33±17.85

项目	例数	1,25（OH）₂D₃ （ng/mL）	IL-10 （pg/mL）	IL-17 （pg/mL）
治疗前	23	19.34±5.91	16.82±3.57	134.72±27.49
治疗后	23	26.39±6.94	22.78±4.86	107.52±24.03
t值		5.281	4.973	5.017
P值		<0.05	<0.05	<0.05

项目	例数	1,25（OH）₂D₃ （ng/mL）	IL-10 （pg/mL）	IL-17 （pg/mL）
治疗前	23	19.34±5.91	16.82±3.57	134.72±27.49
治疗后	23	26.39±6.94	22.78±4.86	107.52±24.03
t值		5.281	4.973	5.017
P值		<0.05	<0.05	<0.05

Changes of serum 1,25-dihydroxyvitamin D₃,IL-17 and IL-10 in patients with osteoarthritis

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