Influence of Del-1 on ICAM-1 expression of eosinophils in atopic allergy children

doi:10.3969/j.issn.1673-8640.2018.01.013

Abstract

Abstract:

Objective To evaluate the influence of developmental endothelial locus-1 （Del-1） on intercellular cell adhesion molecule-1 （ICAM-1）expression of eosinophils in atopic allergy children.Methods Peripheral blood samples were collected from atopic allergy children （35 cases）,and eosinophils were isolated by magnetic-activated cell sorting （MACS）. ICAM-1 expression was determined by reverse transcription polymerase chain reaction （PCR） after administrating Del-1,anti-Del-1 antibody or interleukin 17 （IL-17）. Results Compared with control group treated with phosphate-buffered saline （PBS）,ICAM-1 expression was down-regulated after administrating Del-1（P<0.05）,and it was up-regulated after administrating anti-Del-1 antibody （P<0.05）. ICAM-1 expression was down-regulated after administrating Del-1 and IL-17 compared with that after administrating IL-17 only （P<0.05）. There was no statistical significance in non-eosinophils after administrating Del-1 or anti-Del-1 antibody （P>0.05）.Conclusions Del-1 can regulate the expression of ICAM-1,indicating that Del-1 can negatively regulate the intercellular adhesion of eosinophils,thereby limiting atopic allergy of children.

Key words: Developmental endothelial locus-1, Intercellular cell adhesion molecule-1, Interleukin 17, Atopic allergy

CLC Number:

R446.63

YAN Shu, ZHANG Hong. Influence of Del-1 on ICAM-1 expression of eosinophils in atopic allergy children[J]. Laboratory Medicine, 2018, 33(1): 67-71.

Figures/Tables 4

References 10

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