›› 2013, Vol. 28 ›› Issue (5): 396-399.DOI: 10.3969/j.issn.1673-8640.2013.05.012

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The changes and clinical significance on the expressions of Th17, Th1 and cytokines in patients with systemic lupus erythematosus

  

  1. 1.Department of Clinical Laboratory,Dalian Municipal Central Hospital,Liaoning Dalian 116033, China; 2. Life Science College, Liaoning Normal University,Liaoning Dalian 116029, China
  • Received:2012-08-27 Revised:2012-10-22 Online:2013-05-15 Published:2013-05-14

Abstract: Objective To investigate the expressions of T helper cell 17 (Th17) , T helper cell 1 (Th1) and cytokines such as interleukin 17 (IL-17), interleukin 23(IL-23) and interferon-gamma (IFN-γ) in peripheral blood from patients with systemic lupus erythematosus(SLE),and their clinical significance. Methods According to SLE disease activity index (SLEDAI),38 patients with SLE were classified into active group(SLEDAI≥10,20 patients) and inactive group(SLEDAI<10,18 patients). Flow cytometry was used to analyze the percentages of Th17 and Th1 in 38 patients with SLE and 20 healthy controls. The expressions of IL-17, IL-23 and IFN-γ were determined by enzyme-linked immunosorbent assay(ELISA),and the results were analyzed statistically. Results The percentage of Th17 in patients with SLE was significantly higher than that in healthy controls(P<0.05),and the percentage of Th17 in active group was higher than that in inactive group(P<0.05), but the expression of Th1 had no statistical significance with that of healthy controls(P>0.05) . The expressions of IL-17, IL-23 and IFN-γ in patients with SLE were higher than those in healthy controls(P<0.05). The expression of Th17 and the levels of IL-17 and IL-23 were positively correlated  with SLEDAI(r=0.537, 0.428 and 0.525, P<0.05).  Conclusions The expression of Th17 and the levels of IL-17, IL-23 and IFN-γ increase in patients with SLE, and Th17 and Th1 may play important roles in the pathogenesis of SLE.

Key words: T helper cell 17, T helper cell 1, Interleukin 17, Interleukin 23, Interferon-gamma, Systemic lupus erythematosus