Abstract:

Objective To investigate the determination and drug resistance gene of methicillin-resistant Staphylococcus aureus （MRSA） isolated from adults in Jiangsu Province Hospital on Integration of Chinese and Western Medicine from 2011 to 2017,and to provide a reference for clinical rational drug use. Methods According to the Clinical and Laboratory Standards Institute （CLSI） guidelines,MRSA were identified. The drug susceptibility was determined by disk diffusion method. The β-lactam resistance gene mecA,aminoglycoside modifying enzyme genes aac（6′）/aph（2″）and aph（3）-Ⅲ,macrolide 23s rRNA methylase gene ermA/C,tetracycline-like ribosomal protective protein gene tetM/K,quinolone resistance gene qnrA/B and disinfectant resistance gene qacA/B were determined by polymerase chain reaction （PCR）. Results The determination rate of MRSA was 31.95%,and the isolates of MRSA were mainly isolated from sputum and wound secretion samples. The top 3 departments were respiratory department,surgery department and intensive care unit. There was no MRSA isolate being resistant to vancomycin,linezolid and tigecycline. There were 0.49% MRSA isolates being resistant to quinupristin. The drug resistance rates to cotrimoxazole,rifampicin,ciprofloxacin,tetracycline,gentamicin,clindamycin and erythromycin were 11.18%,11.57%,21.47%,63.63%,44.31%,59.41% and 62.35%,respectively. The determination rate of mecA was 100%. The determination rates of aac（6′）/aph（2″）,aph（3）-Ⅲ,ermA/C,tetM/K,qnrA/B and qacA/B were 36.67 %,27.94%,68.43%,57.35%,23.63% and 52.45%,respectively.Conclusions From 2011 to 2017,the determination rate of MRAS multidrug resistance gene is high. MRSA shows various degrees of drug resistance to many antibiotics. Drug susceptibility test's results can be used reference for clinical medication.

Key words: Methicillin-resistant Staphylococcus aureus, Multidrug resistance, Gene