Correlation of Staphylococcus aureus drug resistance with drug resistance genes and virulence genes

doi:10.3969/j.issn.1673-8640.2023.04.011

Abstract

Abstract:

Objective To analyze the distribution of drug resistance genes and virulence genes of different drug resistant Staphylococcus aureus，and to investigate the correlation between drug resistance genes，virulence genes and drug resistance. Methods Totally，220 isolates of Staphylococcus aureus isolated in the Second Affiliated Hospital of Wannan Medical College from January 2018 to January 2021 were collected and classified into methicillin-resistant Staphylococcus aureus（MRSA，110 isolates） and methicillin-sensitive Staphylococcus aureus（MSSA，110 isolates）. The drug resistance genes（mecA，mecC and ermA） and virulence gene（SEA） were determined by polymerase chain reaction（PCR）. Results Among the 220 isolates of Staphylococcus aureus，MSSA had a high drug resistance rate to penicillin，there were low drug resistance rates to chloramphenicol，tetracycline，quinolone，sulfonamide and aminoglycoside. MRSA had high drug resistance rates to many types of antibacterial drugs，and the drug resistance rates to oxacillin and penicillin were 100%. Among MRSA isolates，the rates of carrying drug resistance genes and virulence genes were higher than those of MSSA isolates（P<0.01）. The drug resistance of Staphylococcus aureus with mecA drug resistance gene was higher than that of Staphylococcus aureus without mec A（P<0.01）. The drug resistance rates of Staphylococcus aureus with SEA virulence gene and ermA drug resistance gene，except for compound trimethoprim，were higher than those of Staphylococcus aureus without SEA virulence gene and ermA drug resistance gene（P<0.05）. Conclusions Drug resistance genes，mecA，mecC and ermA，and virulence gene SEA are related to the drug resistance of Staphylococcus aureus.

Key words: Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Methicillin-sensitive Staphylococcus aureus, Drug resistance gene, Virulence gene, Drug resistance

CLC Number:

R446.5

FANG Yongmei, ZHANG Yan, XU Xueying, ZHONG Feng. Correlation of Staphylococcus aureus drug resistance with drug resistance genes and virulence genes[J]. Laboratory Medicine, 2023, 38(4): 357-361.

Figures/Tables 5

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基因名称	引物序列（5'~3'）	产物大小/bp
mecA	F：ACGAGTAGATGCTCAATATAA	293
	R：CTTAGTTCTTTAGCGATTGC
mecC	F：GAAAAAAAGGCTTAGAACGCCTC	138
	R：GAGATCTTTTCCGTTTTCAGC
SEA	F：GGTTATCAATGTGCGGGTGG	232
	R：CGGCACTTTTTTCTCTTCGG
ermA	F：GTTCAAGAACAATCACAGAG	533
	R：GGATCAGGAAAAGGACATTTTAC

基因名称	引物序列（5'~3'）	产物大小/bp
mecA	F：ACGAGTAGATGCTCAATATAA	293
	R：CTTAGTTCTTTAGCGATTGC
mecC	F：GAAAAAAAGGCTTAGAACGCCTC	138
	R：GAGATCTTTTCCGTTTTCAGC
SEA	F：GGTTATCAATGTGCGGGTGG	232
	R：CGGCACTTTTTTCTCTTCGG
ermA	F：GTTCAAGAACAATCACAGAG	533
	R：GGATCAGGAAAAGGACATTTTAC

抗菌药物	MRSA/%	MSSA/%	χ²值	P值
苯唑西林	100.00	0	220.000	0.000
青霉素	100.00	86.36	16.098	0.000
红霉素	77.27	47.27	21.069	0.000
克林霉素	64.55	35.45	18.618	0.000
利福平	17.27	0	20.796	0.000
氯霉素	33.66	12.73	13.503	0.000
四环素	53.66	7.27	55.821	0.000
莫西沙星	55.45	10.91	49.224	0.000
万古霉素	0	0
利奈唑胺	0	0
环丙沙星	57.29	24.55	24.369	0.000
左氧氟沙星	67.27	30.91	29.101	0.000
复方磺胺甲噁唑	13.64	2.73	8.713	0.003
奎奴普丁-达福普丁	0	0
庆大霉素	32.73	6.36	24.310	0.000

抗菌药物	MRSA/%	MSSA/%	χ²值	P值
苯唑西林	100.00	0	220.000	0.000
青霉素	100.00	86.36	16.098	0.000
红霉素	77.27	47.27	21.069	0.000
克林霉素	64.55	35.45	18.618	0.000
利福平	17.27	0	20.796	0.000
氯霉素	33.66	12.73	13.503	0.000
四环素	53.66	7.27	55.821	0.000
莫西沙星	55.45	10.91	49.224	0.000
万古霉素	0	0
利奈唑胺	0	0
环丙沙星	57.29	24.55	24.369	0.000
左氧氟沙星	67.27	30.91	29.101	0.000
复方磺胺甲噁唑	13.64	2.73	8.713	0.003
奎奴普丁-达福普丁	0	0
庆大霉素	32.73	6.36	24.310	0.000

基因名称	MRSA（110株）		MSSA（110株）		χ²值	P值
基因名称	阳性/[株（%）]	阴性/[株（%）]	阳性/[株（%）]	阴性/[株（%）]	χ²值	P值
mecA	110（100.00）	0（0）	5（4.55）	105（95.45）	76.364	0.000
ermA	65（59.09）	45（40.91）	8（7.27）	102（92.73）	66.609	0.000
SEA	93（84.55）	17（15.45）	17（15.45）	93（84.55）	105.018	0.000
mecC	39（35.45）	71（64.55）	12（10.91）	98（89.09）	18.608	0.000