Abstract: Objective To establish the reference intervals of serum glutathione reductase（GR） in apparently healthy adults. Methods Serum GR activities of 430 healthy adults,including 205 males and 225 females,were determined,and the age- and sex-related reference intervals were determined according to the Clinical and Laboratory Standards Institute（CLSI） C28-A3 document. Results After eliminating 5 outliers,the GR activities showed a non-Gaussian distribution in serum of healthy adults. Serum GR activity in males was 53（47-57） U/L,which was slightly higher than that in females [51（47-55） U/L]（Z=-2.156,P<0.05）. Correlation analysis showed that serum GR activities had positive correlations with age,alanine aminotransferase（ALT）,aspartate aminotransferase（AST）,gamma glutamyltransferase（GGT）,alkaline phosphatase（ALP）,glucose（Glu）,triglyceride（TG）,total cholesterol（TC） and low-density lipoprotein cholesterol（LDL-C）and a negative correlation with high-density lipoprotein cholesterol（HDL-C）. There was no correlation between serum GR activities and total bilirubin（TB）. The GR activities in males showed no statistical significance among all the age groups,and the subjects were combined into male group. The GR activities in ≥51-year-old females were higher than those in 18-50-year-old females,so that the subjects were regrouped into 18-50-year-old and ≥51-year-old groups. The reference intervals of serum GR activities in healthy adults were 36-72 U/L for males,38-65 U/L（18-50-year-old） and 45-67 U/L（≥51-year-old） for females by percentiles. Conclusions The reference intervals of serum GR in apparently healthy adults have initially established,which provides a reference for the clinical application of GR.

Key words: Glutathione reductase, Apparently healthy adult, Reference interval