Diagnostic roles of serum syndecan-1 and eNAMPT levels in patients with severe pneumonia complicated with sepsis

doi:10.3969/j.issn.1673-8640.2025.09.005

Abstract

Abstract:

Objective To investigate the diagnostic roles of serum syndecan-1 and extracellular nicotinamide phosphoribosyltransferase（eNAMPT）levels in patients with severe pneumonia（SP）complicated with sepsis. Methods A total of 168 SP patients admitted to Zhangjiakou First Hospital from June 2019 to October 2023 were enrolled. They were classified into SP with sepsis group（52 cases）and SP without sepsis group（116 cases）based on whether they developed sepsis after admission. The SP with sepsis group was further classified into sepsis group（38 cases）and septic shock group（14 cases）according to the sequential organ failure assessment（SOFA）score. Additionally，they were classified into survival group（35 cases）and death group（17 cases）based on the prognosis. Serum syndecan-1 and eNAMPT levels were determined by enzyme-linked immunosorbent assay（ELISA）. Pearson correlation analysis was used to evaluate the correlation between syndecan-1，eNAMPT levels and inflammatory indicators. Multivariate Logistic regression analysis was used to assess the factors influencing sepsis in SP. Receiver operating characteristic（ROC）curve was used to analyze the diagnostic value of serum syndecan-1 and eNAMPT levels for sepsis in SP. Results The levels of serum C-reactive protein（CRP），interleukin-6（IL-6），cardiac troponin I（cTnI），procalcitonin（PCT），white blood cell（WBC）count，syndecan-1 and eNAMPT in SP with sepsis group were higher than those in SP without sepsis group（P<0.001）. The levels of serum syndecan-1 and eNAMPT in septic shock group were higher than those in sepsis group（P<0.001）. Compared with survival group，the levels of serum syndecan-1 and eNAMPT in death group were increased（P<0.001）. Serum syndecan-1 and eNAMPT levels were positively correlated with CRP，IL-6，cTnI，PCT and WBC count（P<0.001）. Serum syndecan-1，eNAMPT，CRP，IL-6，cTnI，PCT and WBC count were all risk factors for sepsis in SP（P<0.05）. The areas under curves（AUC）of serum syndecan-1，eNAMPT single and combined determinations for diagnosing sepsis in SP were 0.660，0.812 and 0.844，respectively. The AUC of the combined determination determination diapnosis medel for SP with sepsis based on multivariate Logistic regression analysis was 0.981. Conclusions The levels of serum syndecan-1 and eNAMPT in SP patients with sepsis are increased，which are related to the severity and prognosis. The combined determination with conventional inflammatory indicators has a better diagnostic efficiency for sepsis in SP.

Key words: Syndecan-1, Extracellular nicotinamide phosphoribosyltransferase, Severe pneumonia, Sepsis

CLC Number:

R446.1

ZHAO Lixiao, YANG Xiaoting, LI Dan, ZHANG Nan, SU Haiyan, WANG Yan. Diagnostic roles of serum syndecan-1 and eNAMPT levels in patients with severe pneumonia complicated with sepsis[J]. Laboratory Medicine, 2025, 40(9): 856-861.

Figures/Tables 7

References 25

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组别	例数	性别		年龄/岁	体重指数/（kg·m^-2）	吸烟史/[例（%）]	饮酒史/[例（%）]
组别	例数	男/[例（%）]	女/[例（%）]	年龄/岁	体重指数/（kg·m^-2）	吸烟史/[例（%）]	饮酒史/[例（%）]
并发脓毒症组	52	31（59.62）	21（40.38）	60.35±5.47	22.16±1.59	15（28.85）	9（17.31）
未并发脓毒症组	116	53（45.69）	63（54.31）	60.28±5.79	21.87±2.13	26（22.41）	24（20.69）
统计值		2.785		0.074	0.878	0.805	0.260
P值		0.095		0.941	0.381	0.370	0.610
组别	糖尿病史/[例（%）]		高血压史/[例（%）]		CRP/（mg·L^-1）	IL-6/（ng·L^-1）	cTnI/（ng·mL^-1）
并发脓毒症组	9（17.31）		6（11.54）		48.73±15.26	96.13±27.68	1.94±0.54
未并发脓毒症组	11（9.48）		6（5.17）		19.23±5.66	41.21±12.66	0.23±0.07
统计值	2.096		2.194		18.258	17.681	33.602
P值	0.148		0.139		<0.001	<0.001	<0.001
组别	PCT/（ng·mL^-1）		WBC计数/（×10⁹L^-1）		syndecan-1/（μg·L^-1）		eNAMPT/（ng·mL^-1）
并发脓毒症组	9.35±2.44		17.59±4.62		67.59±19.46		28.37±7.65
未并发脓毒症组	3.42±0.67		11.36±2.47		51.24±16.38		21.43±6.12
统计值	24.289		11.368		5.636		6.274
P值	<0.001		<0.001		<0.001		<0.001

组别	例数	性别		年龄/岁	体重指数/（kg·m^-2）	吸烟史/[例（%）]	饮酒史/[例（%）]
组别	例数	男/[例（%）]	女/[例（%）]	年龄/岁	体重指数/（kg·m^-2）	吸烟史/[例（%）]	饮酒史/[例（%）]
并发脓毒症组	52	31（59.62）	21（40.38）	60.35±5.47	22.16±1.59	15（28.85）	9（17.31）
未并发脓毒症组	116	53（45.69）	63（54.31）	60.28±5.79	21.87±2.13	26（22.41）	24（20.69）
统计值		2.785		0.074	0.878	0.805	0.260
P值		0.095		0.941	0.381	0.370	0.610
组别	糖尿病史/[例（%）]		高血压史/[例（%）]		CRP/（mg·L^-1）	IL-6/（ng·L^-1）	cTnI/（ng·mL^-1）
并发脓毒症组	9（17.31）		6（11.54）		48.73±15.26	96.13±27.68	1.94±0.54
未并发脓毒症组	11（9.48）		6（5.17）		19.23±5.66	41.21±12.66	0.23±0.07
统计值	2.096		2.194		18.258	17.681	33.602
P值	0.148		0.139		<0.001	<0.001	<0.001
组别	PCT/（ng·mL^-1）		WBC计数/（×10⁹L^-1）		syndecan-1/（μg·L^-1）		eNAMPT/（ng·mL^-1）
并发脓毒症组	9.35±2.44		17.59±4.62		67.59±19.46		28.37±7.65
未并发脓毒症组	3.42±0.67		11.36±2.47		51.24±16.38		21.43±6.12
统计值	24.289		11.368		5.636		6.274
P值	<0.001		<0.001		<0.001		<0.001

组别	例数	syndecan-1/（μg·L^-1）	eNAMPT/（ng·mL^-1）
脓毒症组	38	62.64±11.58	26.16±6.54
脓毒性休克组	14	81.03±15.84	34.38±7.15
t值		4.601	3.922
P值		<0.001	<0.001

组别	例数	syndecan-1/（μg·L^-1）	eNAMPT/（ng·mL^-1）
脓毒症组	38	62.64±11.58	26.16±6.54
脓毒性休克组	14	81.03±15.84	34.38±7.15
t值		4.601	3.922
P值		<0.001	<0.001

组别	例数	syndecan-1/ （μg·L^-1）	eNAMPT/（ng·mL^-1）
生存组	35	55.86±12.24	24.37±5.04
死亡组	17	91.74±25.37	36.61±7.64
t值		6.917	6.905
P值		<0.001	<0.001