Roles of IP-10 and MCP-1 in cerebrospinal fluid of children with viral encephalitis

doi:10.3969/j.issn.1673-8640.2024.05.007

Abstract

Abstract:

Objective To investigate the roles of interferon-inducible protein-10（IP-10） and monocyte chemotactic protein-1（MCP-1） in the cerebrospinal fluid of children with viral encephalitis. Methods Totally，60 children with viral encephalitis（viral encephalitis group） were enrolled from Xingtai People's Hospital from July 2019 to December 2020，which included 26 mild cases and 34 severe cases，and 40 children with fever excluding intracranial infection were enrolled as control group. The levels of IP-10，MCP-1 and tumor necrosis factor-alpha（TNF-α） in cerebrospinal fluid were determined by enzyme-linked immunosorbent assay. The correlations of IP-10 and MCP-1 with TNF-α levels were evaluated by Pearson correlation analysis. Results The levels of cerebrospinal fluid IP-10，MCP-1 and TNF-α in viral encephalitis group were higher than those in control group（P<0.001）. The levels of cerebrospinal fluid IP-10，MCP-1 and TNF-α in severe group were higher than those in mild group（P<0.05）. The cerebrospinal fluid levels of IP-10 and MCP-1 were positively correlated with TNF-α levels in viral encephalitis group（r values were 0.742 and 0.696，P<0.05）. Conclusions IP-10 and MCP-1 are correlated with the severity of viral encephalitis in children and can be used as one of the indicators of disease surveillance.

Key words: Interferon-inducible protein-10, Monocyte chemotactic protein-1, Viral encephalitis, Children

CLC Number:

R446.1

ZHANG Na, LI Jing, YAN Xiaojing, ZHANG Lan, LIU Weixiao. Roles of IP-10 and MCP-1 in cerebrospinal fluid of children with viral encephalitis[J]. Laboratory Medicine, 2024, 39(5): 454-457.

Figures/Tables 3

References 16

[1]	COSTA B K D, SATO D K. Viral encephalitis:a practical review on diagnostic approach and treatment[J]. J Pediatr(Rio J), 2020, 96(Suppl 1):12-19.
[2]	CARBO E C, BUDDINGH E P, KARELIOTI E, et al. Improved diagnosis of viral encephalitis in adult and pediatric hematological patients using viral metagenomics[J]. J Clin Virol, 2020, 130:104566.
[3]	范伟光, 苏苗苗, 孟娟, 等. AIDS患者抗病毒治疗中IP-10水平的变化及临床意义[J]. 检验医学, 2022, 37(3):221-225. DOI
[4]	胡艮娣, 王晓泉, 陈祥, 等. 趋化因子IP-10在介导病毒感染性疾病中的研究进展[J]. 病毒学报, 2019, 35(4):672-678.
[5]	SINGH S, DIXIT A, RAVICHANDIRAN V. MCP-1:function,regulation,and involvement in disease[J]. Int Immunopharmacol, 2021, 101(Pt B):107598.
[6]	何梅, 尚彪, 杨雪利, 等. 单核细胞趋化蛋白-1、可溶性细胞黏附分子-1诊断新生儿缺氧缺血性脑病的价值[J]. 实用临床医药杂志, 2022, 26(6):47-50.
[7]	江载芳, 申昆玲, 沈颖. 诸福棠实用儿科学[M]. 8版. 北京: 人民卫生出版社, 2015.
[8]	杜开先, 胡会会, 李林, 等. 脑脊液中纤维连接蛋白、血管内皮生长因子及乳酸脱氢酶水平在儿童细菌性脑膜炎和病毒性脑炎中的诊断价值[J]. 实用临床医药杂志, 2021, 25(11):26-30.
[9]	ZHANG W, HUANG Z, HUANG M, et al. Predicting severe enterovirus 71-infected hand,foot,and mouth disease:cytokines and chemokines[J]. Mediators Inflamm, 2020, 2020:9273241.
[10]	YGBERG S, FOWLER Å, WICKSTRÖM R. Age-related changes in the inflammatory responses to viral infections in the central nervous system during childhood[J]. Pediatr Res, 2022, 91(1):204-208.
[11]	LIU J, LI S, CAI C, et al. Cerebrospinal fluid chemokine patterns in children with enterovirus 71-related encephalitis[J]. Sci Rep, 2018, 8(1):1658. DOI PMID
[12]	温世斌, 夏家敏, 郭百海, 等. 脑脊液IP-10、IFN-α、Eotaxin水平对病毒性脑膜炎诊断价值的研究[J]. 中国病原生物学杂志, 2020, 15(9):1093-1097.
[13]	周芳伊, 李媛媛, 王诚伍, 等. 1型单纯疱疹病毒性脑炎患儿的炎性细胞因子水平[J]. 临床神经病学杂志, 2018, 31(5):321-323.
[14]	周瑜, 曾艳平, 罗瑛, 等. MMP-9及MCP-1在单纯疱疹病毒性脑炎患者脑脊液中的表达及意义[J]. 临床急诊杂志, 2020, 21(3):188-191.
[15]	董进浪, 谭炜炜, 杨春旺. 流行性乙型脑炎患儿血清及脑脊液中白细胞家族成员、MMP-9及MCP-1的表达水平及临床意义[J]. 国际医药卫生导报, 2019, 25(13):2101-2105.
[16]	THEPMANKORN P, BACH J, LASFAR A, et al. Cytokine storm induced by SARS-CoV-2 infection:the spectrum of its neurological manifestations[J]. Cytokine, 2021, 138:155404.

组别	例数	性别		年龄/岁	病程/d	IP-10/ （pg·mL^-1）	MCP-1/ （pg·mL^-1）	TNF-α/ （pg·mL^-1）
组别	例数	男/例	女/例	年龄/岁	病程/d	IP-10/ （pg·mL^-1）	MCP-1/ （pg·mL^-1）	TNF-α/ （pg·mL^-1）
病毒性脑炎组	60	33	27	6.02±1.34	1.84±0.55	169.84±38.31	192.91±46.79	36.34±6.57
对照组	40	21	19	6.45±1.02	1.90±0.59	117.40±27.10	138.56±31.90	10.97±2.18
统计值		0.060		1.723	0.519	7.492	6.414	23.539
P值		0.806		0.088	0.605	<0.001	<0.001	<0.001

组别	例数	性别		年龄/岁	病程/d	IP-10/ （pg·mL^-1）	MCP-1/ （pg·mL^-1）	TNF-α/ （pg·mL^-1）
组别	例数	男/例	女/例	年龄/岁	病程/d	IP-10/ （pg·mL^-1）	MCP-1/ （pg·mL^-1）	TNF-α/ （pg·mL^-1）
病毒性脑炎组	60	33	27	6.02±1.34	1.84±0.55	169.84±38.31	192.91±46.79	36.34±6.57
对照组	40	21	19	6.45±1.02	1.90±0.59	117.40±27.10	138.56±31.90	10.97±2.18
统计值		0.060		1.723	0.519	7.492	6.414	23.539
P值		0.806		0.088	0.605	<0.001	<0.001	<0.001

组别	例数	IP-10/（pg·mL^-1）	MCP-1/（pg·mL^-1）	TNF-α/（pg·mL^-1）
轻症组	26	148.83±32.68	173.72±31.46	26.48±5.22
重症组	34	185.91±41.32	207.58±38.02	43.89±6.37
t值		3.761	3.677	11.323
P值		<0.001	0.001	<0.001

组别	例数	IP-10/（pg·mL^-1）	MCP-1/（pg·mL^-1）	TNF-α/（pg·mL^-1）
轻症组	26	148.83±32.68	173.72±31.46	26.48±5.22
重症组	34	185.91±41.32	207.58±38.02	43.89±6.37
t值		3.761	3.677	11.323
P值		<0.001	0.001	<0.001