Abstract:

Objective To understand the epidemiological characteristics of Epstein-Barr virus（EBV） infection in inpatients from Hebei Children's Hospital,and to provide a reference for the diagnosis and prevention of EBV infection in children. Methodse The whole blood samples of children with EBV infection in Hebei Children's Hospital from January to December in 2017 were collected,and anti-viral capsid antigen（VCA） IgG and IgM antibodies,anti-early antigen（EA） antibody and anti-nuclear antigen-1（NA1） IgG antibody were determined by enzyme-linked immunosorbent assay（ELISA）. The antibody spectrum was analyzed. According to the determination results of 4 kinds of EBV antibodies,the primary infection group（anti-VCA-IgM positive,anti-NA1-IgG negative,anti-VCA-IgG positive or negative,anti-EA-IgG positive or negative）,sub-acute infection group （anti-VCA-IgG positive,anti-VCA-IgM positive or negative,anti-NA1-IgG positive or negative,anti-EA-IgG positive or negative）,previous infection group （anti-NA1-IgG positive,anti-VCA-IgG positive or negative,the rest negative） and uninfected group（all antibodies negative）. The positive rate of each group was analyzed according to age,season and sex. Results The antibody spectrums of 3 257 （73.17%） of 4 451 samples met the set requirements,indicating EBV infection,of which 380 cases were primary infection（8.54%）,616 cases of sub-acute infection（13.84%） and 2 261 cases of previous infection（50.80%）. There was statistical significance in the primary positive rate among different age groups（P<0.05）. The positive rate of pre-school age group（>3 years old） was the highest（P<0.05）. The positive rates of different months had statistical significance（P<0.05）,and the positive rate of EBV in July was higher than those in the other months（P<0.05）. There was no statistical significance in the EBV infection rate between females and males（P>0.05）. The disease spectrum of 380 cases of primary infection was mainly hematological diseases （infectious mononucleosis,acute agranulocytosis,thrombocytopenic purpura,EBV-associated hemophilia syndrome）,of which infectious mononucleosis was a common clinical disease;followed by respiratory diseases （acute bronchitis,herpes angina,acute tonsillitis）;the other diseases included neurological diseases and bloodstream infection,nephrotic syndrome and Kawasaki disease. Conclusions The positive rate of EBV in children in Hebei Children's Hospital varies with ages and months. Among the cases of primary infection,the main disease is hematological disease. The hospital should make corresponding preventive measures according to the epidemiological characteristics.

Key words: Epstein-Barr virus, Epidemiology, Children