Abstract:

Objective To compare different detection methods of severe acute respiratory syndrome coronavirus 2（SARS-CoV-2） IgM and IgG antibodies in the diagnosis of corona virus disease 2019（COVID-19）. Methodse Serum samples were collected from 25 patients diagnosed as COVID-19,and 27 patients without SARS-CoV-2 infection were enrolled as control group. SARS-CoV-2 IgM and IgG antibodies were detected by chemiluminescence and colloidal gold method. The procalcitonin（PCT）,C-reactive protein（CRP） and ferritin were also determined. Results The sensitivities of SARS-CoV-2 IgM and IgG antibodies by chemiluminescence were 48% and 56%,respectively,while those by colloidal gold method were 88% and 76%,respectively. The specificities were both 100% for the 2 methods. The total coincidence rates of IgM and IgG antibodies between the 2 methods were 68.9% and 73.3%,respectively. Serum PCT levels were increased in 36% COVID-19 patients,serum CRP levels were increased in 72% COVID-19 patients,and serum ferritin were increased in 84% COVID-19 patients. Conclusions The clinical performance of different detection methods of SARS-CoV-2 IgM and IgG antibodies are varied,which should be considered comprehensively in the clinical diagnosis of COVID-19.

Key words: Severe acute respiratory syndrome coronavirus 2, Immunoglobulin, Antibody, Chemiluminescence, Colloidal gold method, Corona virus disease 2019