Influence of hemolysis on serum glucose determination according to CLSI EP7-A2

doi:10.3969/j.issn.1673-8640.2018.05.007

Abstract

Abstract:

Objective To evaluate the influence of hemolysis on serum glucose （Glu） determination by hexokinase（HK）assay. Methods The hemoglobin （Hb） was identified as an interfering substance by paired-difference experiment according to the Clinical and Laboratory Standards Institute （CLSI） EP7-A2 document. The correlation between Hb level and interference was evaluated by dose-effect experiment. Results Paired-difference experiment showed that Hb at 5 g/L could influence high-level and middle-level Glu determinations. Dose-effect experiment with 5 levels of Hb showed that there existed non-linear interference in Glu determination. The non-linear equations were Y=6.496+0.160 5X-0.013 10X² for middle-level Glu determination （r²=0.983） and Y=10.13+0.211 0X-0.017 83X² for high-level Glu determination （r²=0.973）. Conclusions There exists interference for Glu determination from hemolytic samples by HK assay. It should avoid hemolytic samples if necessary. If another sample is not available,the hemolysis degree and potential interference should be attached to the laboratory report sheets for clinical reference.

Key words: Glucose, Hexokinase assay, Hemolysis, Paired-difference experiment, Dose-effect experiment

CLC Number:

R446.1

SHEN Jianjiang, FU Buqing, ZHAO Chun, XU Tian. Influence of hemolysis on serum glucose determination according to CLSI EP7-A2[J]. Laboratory Medicine, 2018, 33(5): 404-406.

Figures/Tables 3

References 8

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编号	Hb（g/L）	中浓度Glu（mmol/L）			高浓度Glu（mmol/L）
编号	Hb（g/L）	第1次	第2次	第3次	第1次	第2次	第3次
1	0.00	6.46	6.50	6.50	10.17	10.17	10.07
2	1.25	6.71	6.68	6.69	10.31	10.31	10.37
3	2.50	6.82	6.82	6.81	10.53	10.58	10.60
4	3.75	6.90	6.86	6.92	10.68	10.63	10.67
5	5.00	6.96	6.97	7.01	10.70	10.75	10.75

编号	Hb（g/L）	中浓度Glu（mmol/L）			高浓度Glu（mmol/L）
编号	Hb（g/L）	第1次	第2次	第3次	第1次	第2次	第3次
1	0.00	6.46	6.50	6.50	10.17	10.17	10.07
2	1.25	6.71	6.68	6.69	10.31	10.31	10.37
3	2.50	6.82	6.82	6.81	10.53	10.58	10.60
4	3.75	6.90	6.86	6.92	10.68	10.63	10.67
5	5.00	6.96	6.97	7.01	10.70	10.75	10.75

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