Abstract:

Objective To investigate the feasibility of using glucose（Glu） reference method to determine the target value for external quality assessment（EQA） samples. Methods The value of Glu was assigned to the samples of EQA in Shanghai using isotope dilution gas chromatography tandem mass spectrometry（ID-GC/MS） which was recommended by the Joint Committee for Traceability in Laboratory Medicine（JCTLM）. The data was used as the target value for statistical analysis of the feedback results of participating laboratories in the 2nd EQA of 2018. Results The data of the 2nd EQA was from 509 laboratories in 2018. When the target value was average from all laboratories,setting 7% as allowable total error,the failure rates of EQA samples were 2.16%,2.95%,3.34%,3.14% and 1.77%,respectively. However,the failure rates were 2.75%,3.14%,2.75%,7.86% and 2.16%,respectively,using the value assigned by reference method as target value. Conclusions The quality assessment institutions should pay attention to the quality of EQA samples,and clinical laboratories should pay attention to the reliability of Glu measurement at low concentration.

Key words: Glucose, Reference method, External quality assessment