Application of candidate reference method of apolipoprotein A1 and apolipoprotein B in external quality assessment

doi:10.3969/j.issn.1673-8640.2021.03.006

Abstract

Abstract:

Objective To investigate the application of candidate reference method of apolipoprotein（apo） A1 and apo B in external quality assessment（EQA）. Methods Five levels of frozen human serum products were used for EQA samples. Immunoturbidimetry （ITA）was used for the detection. EQA samples were quantified by isotope dilution liquid chromatography-tandem mass spectrometry（ID-LC-MS/MS）. Samples underwent denaturing,alkalization and tryps in digestion,and were then separated by C18 column and quantified by positive ion mode and multiple reaction monitoring. Results ITA was used in 340 participants. As for apo A1,ID-LC-MS/MS gave values lower than the median of ITA for EQA2-EQA4,but not for EQA1 and EQA5. As for apo B,except EQA4,all ID-LC-MS/MS values were lower than the median of ITA. Conclusions Candidate reference method based on ID-LC-MS/MS can offer application value of assessment based on target value.

Key words: Apolipoprotein A1, Apolipoprotein B, Candidate reference method, External quality assessment

CLC Number:

R446

LI Qing, JU Yi, TANG Liping, JIN Zhonggan, SUN Hewei. Application of candidate reference method of apolipoprotein A1 and apolipoprotein B in external quality assessment[J]. Laboratory Medicine, 2021, 36(3): 259-262.

Figures/Tables 4

References 13

[1]	CONTOIS J H, DELATOUR V. Apolipoprotein B measurement:need for standardization[J]. J Clin Lipidol, 2018,12(2):264-265. DOI URL PMID
[2]	JELLINGER P S, HANDELSMAN Y, ROSENBLIT P D, et al. American association of clinical endocrinologists and american college of endocrinology guidelines for management of dyslipidemia and prevention of cardiovascular disease[J]. Endocr Pract, 2017,23(Suppl 2):1-87. URL PMID
[3]	VAN DEN BROEK I, SMIT N P, ROMIJN F P, et al. Evaluation of interspecimen trypsin digestion efficiency prior to multiple reaction monitoring-based absolute protein quantification with native protein calibrators[J]. J Proteome Res, 2013,12(12):5760-5774. DOI URL PMID
[4]	SMIT N P, ROMIJN F P, VAN DEN BROEK I, et al. Metrological traceability in mass spectrometry-based targeted protein quantitation:a proof-of-principle study for serum apolipoproteins A-I and B100[J]. J Proteomics, 2014,109:143-161. URL PMID
[5]	VAN DEN BROEK I, ROMIJN F P, NOUTA J, et al. Automated multiplex LC-MS/MS assay for quantifying serum apolipoproteins A-I,B,C-I,C-II,C-III,and E with qualitative apolipoprotein E phenotyping[J]. Clin Chem, 2016,62(1):188-197. URL PMID
[6]	李卿, 居漪, 孙贺伟, 等. ID-LC-MS检测血清载脂蛋白A-I和B载脂蛋白的多肽释放研究[J]. 中华检验医学杂志, 2018,11(41):870-874.
[7]	PAGANO S, CARBONE F, BURGER F, et al. Anti-apolipoprotein A-1 auto-antibodies as active modulators of atherothrombosis[J]. 2016,116(3):554-564.
[8]	DITTRICH J, ADAM M, MAAS H, et al. Targeted on-line SPE-LC-MS/MS assay for the quantitation of 12 apolipoproteins from human blood[J]. Proteomics, 2018,18(3-4). DOI URL PMID
[9]	PARKS B A, SCHIELTZ D M, ANDREWS M L, et al. High throughput quantification of apolipoproteins A-I and B-100 by isotope dilution MS targeting fast trypsin releasable peptides without reduction and alkylation[J]. Proteomics. Clin Appl, 2017,11(7-8):1600128.
[10]	PANTEGHINI M, CERIOTTI F, JONES G, et al. Strategies to define performance specifications in laboratory medicine：3 years on from the Milan Strategic Conference[J]. Clin Chem Lab Med, 2017,55(12):1849-1856. DOI URL PMID
[11]	冯仁丰. 临床实验室分析质量目标的共识(下)[J]. 检验医学, 2016,31(2):83-86.
[12]	中国成人血脂异常防治指南修订联合委员会. 中国成人血脂异常防治指南(2016年修订版)[J]. 中国循环杂志, 2016,31(10):937-950.
[13]	DÍAZ-GARZÓN J, FERNÁNDEZ-CALLE P, MINCHINELA J, et al. Biological variation data for lipid cardiovascular risk assessment biomarkers. A systematic review applying the biological variation data critical appraisal checklist(BIVAC)[J]. Clin Chim Act, 2019,495:467-475.

蛋白名称	肽段用途	肽段（简写）	一级结构中位置	母离子/（m/z）	子离子/（m/z）	碰撞能量/V
apo A1	定量	VQPYLDDFQK、	121-130	626.8	1025.5	26
		SIL^①-VQPYLDDFQK		629.7	1028.5	18
	定性	AKPALEDLR	231-239	506.8	813.4	24
apo B	定量	TEVIPPLIENR、	950-960	640.9	838.5	30
		SIL^①-TEVIPPLIENR		644	841.5	25
	定性	TGISPLALIK	220-229	506.8	741.5	30

蛋白名称	肽段用途	肽段（简写）	一级结构中位置	母离子/（m/z）	子离子/（m/z）	碰撞能量/V
apo A1	定量	VQPYLDDFQK、	121-130	626.8	1025.5	26
		SIL^①-VQPYLDDFQK		629.7	1028.5	18
	定性	AKPALEDLR	231-239	506.8	813.4	24
apo B	定量	TEVIPPLIENR、	950-960	640.9	838.5	30
		SIL^①-TEVIPPLIENR		644	841.5	25
	定性	TGISPLALIK	220-229	506.8	741.5	30

项目	EQA1		EQA2		EQA3		EQA4		EQA5
项目	中位数/（g/L）	变异系数/ %	中位数/（g/L）	变异系数/ %	中位数/（g/L）	变异系数/ %	中位数/（g/L）	变异系数/ %	中位数/（g/L）	变异系数/ %
apo A1	2.17	6.86	1.78	7.57	2.54	7.71	0.90	8.49	1.46	8.21
apo B	1.39	17.08	1.12	19.46	1.56	16.18	0.54	16.67	0.92	22.28

项目	EQA1		EQA2		EQA3		EQA4		EQA5
项目	中位数/（g/L）	变异系数/ %	中位数/（g/L）	变异系数/ %	中位数/（g/L）	变异系数/ %	中位数/（g/L）	变异系数/ %	中位数/（g/L）	变异系数/ %
apo A1	2.17	6.86	1.78	7.57	2.54	7.71	0.90	8.49	1.46	8.21
apo B	1.39	17.08	1.12	19.46	1.56	16.18	0.54	16.67	0.92	22.28

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