Abstract:

Objective To study the quality of mass spectrometry（MS） bacterium identification of medical laboratories in Shanghai and several regions. Methods The feedback results of MS bacterium identification external quality assessment in Shanghai Center for Clinical Laboratory（SCCL） from 2015 to 2017 were collected,and the consistency of bacterium identification was analyzed. Results The number of laboratories participating SCCL microbial MS bacterium identification external quality assessment increased from 10 in 2015 to 40 in 2019. The qualification rate remained 100.0% during the 5 years. A total of 50 isolates of bacteria were delivered（covering 27 genera and 43 species）. The consistency rate was 100.0%. The isolates with incorrect identification results included Aeromonas veronii,Aeromonas sobria,Staphylococcus intermedius,Staphylococcus epidermidis and Streptococcus pneumoniae,and the consistency rates were 60%,94.4%,95.5%,89.7% and 92.5%,respectively. Conclusions The number of laboratories,mainly in Grade 3 hospitals,setting MS bacterium identification in Shanghai and several regions,increases year by year. The overall detection ability and accuracy are good and high,while some laboratories' performance still should be improved. Quality control in clinical laboratories is essential to assure accuracy.

Key words: Microbial mass spectrum, External quality assessment, Quality control