Overview of detection methods for Mycoplasma in cell preparations

doi:10.3969/j.issn.1673-8640.2022.07.017

Abstract

Abstract:

Mycoplasma is a common exogenous contaminant during cell culturing. There are many detection methods for Mycoplasma contamination. However,due to the fact that the final product of living cell preparations can not be sterilized,the detection method of Mycoplasma is culturing and indicator cell culturing（DNA staining method）,and the detection time is long. The rapid release test of the final cell preparation has certain limitation,so it is necessary to improve the Mycoplasma detection methods for the final cell preparation. This review briefly introduces the morphology,culturing and nucleic acid detection method of Mycoplasma,hoping to provide a reference for the construction of the evaluation system of cell preparation quality monitoring technology.

Key words: Mycoplasma, Cell preparation, Quality control, Methodology

CLC Number:

R375

CHEN Naihan, SA Yalian. Overview of detection methods for Mycoplasma in cell preparations[J]. Laboratory Medicine, 2022, 37(7): 684-687.

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