Abstract:

Objective To prepare quality control materials of Chlamydia trachomatis（CT）DNA,and to evaluate its stability. Methods The weak positive and positive quality control materials wereprepared with pooled secretion samples. The melting stability of the quality control materials was evaluatedby water bath at 37 ℃ and room temperature（22 ℃）. The stability of the quality control materials in freezing storage was evaluated using univariate linear regression model. Results The melting stability evaluation for the weak positive control material showed acceptable results with significant differences（P<0.05）,but positive control material showed acceptable results without significant differences（P>0.05）. The cryopreserved stability evaluation for the weak positive and positive control materials showed that no significant linear trend was found with extended test time（P>0.05）. Conclusion Positive quality control materials of CT DNA have advantages of simple preparation,good stability and low cost,and can be used in laboratory detection. The cryopreserved secretion samples in the laboratory can achieve the beststability when they are melted in 37 ℃ water bath.

Key words: Chlamydia trachomatis, DNA, Quality control materials, Melt, Stability