Abstract: Objective To study the significance of cholesterol levels on erythrocyte membrane and erythrocyte deformability in patient with great area burn. Method The cholesterol level on erythrocyte membrane and the index of erythrocyte deformability were measured by methods of chemically modified electrode and percolation respectively. Results The amount of cholesterol on erythrocyte membrane in patients with great area burn was higher than that in normal controls and the index of erythrocyte deformability in patients with great area burn was lower than that in normal controls. There was highly negative correlation between patients and controls (r=-0.801). Conclusion Erythrocyte membrane cholestrol may be used as a new index for erythrocyte deformability and the state of microcirculation.