Abstract:

Objective To establish the reference ranges for the 4 lipid parameters of adults in Lhasa by indirect method，and to provide a reference for the prevention and treatment of lipid disorders in the population of the plateau region. Methods Healthy individuals aged 18-80 years from Lhasa People's Hospital from October 2023 to October 2024 were enrolled. Serum total cholesterol（TC），triglyceride（TG），low-density lipoprotein cholesterol（LDL-C）and high-density lipoprotein cholesterol（HDL-C）were determined. The groups were classified by gender and age（18-29 years old，30-39 years old，40-49 years old，50-59 years old，60-69 years old，70-80 years old）. The original data were normalized，and outliers were removed using the Turkey method. The 95% reference ranges for the 4 lipid parameters were established using the Hoffmann method. Results The serum TG，TC and LDL-C levels in males were higher than those in females（P<0.001），while the serum HDL-C level was lower in males（P<0.001）. There was statistical significance in serum TG，TC and LDL-C levels between 18-29 years old group，30-39 years old group and 40-80 years old groups（P<0.001），while there was no statistical significance among 40-80 years old groups（P>0.05）. For females，the differences in serum TG and LDL-C levels were not statistically significant except for 60-69 years old group and 70-80 years old group（P>0.05），while the differences were statistically significant among the other age groups（P<0.05）. The serum TC levels showed no statistical significance among 50-59，60-69 and 70-80 years old groups （P>0.05），while significant differences were observed among the other age groups （P<0.05）. There was no statistical significance in serum HDL-C levels among males and females in each age group（P>0.05）. The reference ranges for TG，TC and LDL-C were established for males in 3 age groups（18-29 years old，30-39 years old and 40-80 years old），while the reference ranges for TG and LDL-C were established for females in 5 age groups（18-29 years old，30-39 years old，40-49 years old，50-59 years old and 60-80 years old）. The reference ranges for TC were established for females in 4 age groups（18-29 years old，30-39 years old，40-49 years old and 50-80 years old）. HDL-C reference ranges were established for males and females. The relative deviations between the self-established reference ranges and the current reference ranges for the 4 lipid parameters were calculated，and there were differences of varying degrees with respect to the reference change value（RCV）of adults item. Conclusions There are differences of varying degrees between the reference ranges of the 4 lipid parameters for adults in the plateau region and the current reference ranges. Appropriate reference ranges for lipid parameters should be established for different regions and populations.

Key words: Total cholesterol, Triglyceride, Low-density lipoprotein cholesterol, High-density lipoprotein cholesterol, Reference range, Hoffmann method, Plateau region