Abstract:

Objective To establish the reference ranges for fluorescence-based platelet count（PLT-F）and platelet-related parameters for healthy adults in Wuhan. Methods A total of 1 067 healthy subjects aged ≥18 years who underwent physical examinations at Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology from November 2021 to August 2023 were enrolled. Venous whole blood samples were collected，and the mean platelet volume（MPV），platelet distribution width（PDW），platelet-larger cell ratio（P-LCR），platelet hematocrit（PCT）and immature platelet fraction（IPF）were determined using automatic hematology analyzer. The data were grouped by gender and age for comparison，and the reference ranges were established. Results There was no statistical significance in IPF and PDW between different genders and ages（P>0.05）. The reference ranges were（0.90-9.86）fL and（9.5-18.1）%，respectively. The reference ranges of MPV and P-LCR% for males were（9.11-12.69）fL and（17.90-46.09）%，while those for females were（9.27-13.00）fL and（18.05-48.87）%. The reference ranges of PLT-F and PCT for males in the young，middle-aged and elderly groups were（161-384）×10⁹L^-1，（147-374）×10⁹L^-1 and（118-366）×10⁹L^-1，respectively. For females，the reference ranges of PLT-F and PCT were（149-404）×10⁹L^-1，（157-375）×10⁹L^-1 and（124-356）×10⁹L^-1，respectively. The reference ranges of PCT for males in the young，middle-aged and elderly groups were（0.19-0.36）%，（0.17-0.37）% and（0.15-0.38）%，respectively. For females，the reference ranges of PCT were（0.17-0.43）%，（0.18-0.40）% and（0.15-0.36）%，respectively. The reference ranges were verified and showed a 100% accuracy rate. Conclusions The reference ranges for PLT-F and PLT-related parameters for adults in Wuhan have preliminarily been established.

Key words: Platelet count, Platelet parameter, Reference range, Fluorescence method