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Table of Content
01 March 2001, Volume 16 Issue 03
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微生物学检验论著
Analysis of assay results of antimicrobial zone diameters by sulfomides using six Mueller-Hinton media
2001, 16(03): 133-135.
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Objective Six M-H media were compared for selecting the best M-H in the sulfomides antimicrobialtest. Methods According to NCCLS, paper disks diffusion method were adopetd, 3 standard strains for E. faecalis(ATCC 229212), E.coli(ATCC 25922) and S. aureus(ATCC 25923) each were used to test SMZco 50 times on six M-H media;10 test strains each were used to test SMZco 10 times on six M-H media. Results 50 times inhibitory zone diameters>20 mm when E. faecalis(ATCC 29212) were tested to SMZco by using Difco and Oxoid company M-H media, 50 times inhibitory zone diameters<20 mm when other four M-H media were used. All test results in NCCLS limits when using E.coli (ATCC 25922) and S.aureus(ATCC 25923) to test SMZco on six M-H media. When 10 test strains were used to test SMZco zone diameters, other four M-H media possess different degree double ring phenomena except for Difco and Oxoid company M-H media. Conclusion Difco and Oxoid company M-H media are qualified, other four M-H media are not qualified.
Coagglutination test for detecting V. Cholerae serotype O1
CHEN Zhaopen;YUAN Yinghua;Liu Zhongming
2001, 16(03): 136-137.
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Objective To develop a simple and rapid method for detecting V. cholerae O1. Methods Staphylococcus aureus Cowan I was coated with anti-V. cholerae O1 monoclonal antibodies and a coagglutination test(COA) detecting V. cholerae O1 in 6-h fecal enrichment cultures was tested. Results The detect limit of COA was 4×106 CFU/ml, and detect time was 5 min. When 92 samples were tested, the diagnostic specificity, sensitivity and accuracy of the test, compared with standard culture methods, were 100%、95.5%、97.8%,respectively. Conclusion The COA is simple and less time-consuming, and can be used as a new method for rapid detection of V. Cholerae.
临床检验与血液学论著
The relationship of erythrocyte membrane cholesterol and erythrocyte deformability in patients with great area burn
2001, 16(03): 138-139.
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Objective To study the significance of cholesterol levels on erythrocyte membrane and erythrocyte deformability in patient with great area burn. Method The cholesterol level on erythrocyte membrane and the index of erythrocyte deformability were measured by methods of chemically modified electrode and percolation respectively. Results The amount of cholesterol on erythrocyte membrane in patients with great area burn was higher than that in normal controls and the index of erythrocyte deformability in patients with great area burn was lower than that in normal controls. There was highly negative correlation between patients and controls (r=-0.801). Conclusion Erythrocyte membrane cholestrol may be used as a new index for erythrocyte deformability and the state of microcirculation.
Genotyping of the Duffy blood group
2001, 16(03): 140-141.
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Objective To establish the genotyping method of the Duffy blood group.Methods According to the sequence of cDNA coding for FY glycoprotein,the Fya/Fyb primers were synthesized,and the genotyping method with PCR-SSP was established, The Duffy blood group of 102 unrelated donors in Zhejiang have been investigated by this method.Results Complete correlation between Fy phenotypes and genotypes was obtained in all samples studied. In all of 102 Zhejiang Han populations, 91 were Fya/Fya homozygotes(89.2%), 10 were Fya/Fyb heterozygotes(8.8%),and 1 was Fyb/Fyb homozygote(0.98%).The gene frequency of Fya was 0.9412,compared with that 0.0588 of Fyb.Conclution The genotyping method of Duffy blood group with PCR-SSP was simple and rapid, and suitable for clinical application. The Fya gene was the major gene of Duffy blood group in Zhejiang Han population.
生物化学检验论著
The direct assay for zinc in serum with 5-Br-PADAP
CAO Jianming;WANG Zhayue;ZHOU Tieli
2001, 16(03): 142-143.
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Objective To establish a simple. sensitive and stable method of determination of zinc in serum by UV-spectrophotometry. Methods Determination of serum zinc with 2-(5-bromo-2-pyridylazo)-5-diethylaminophenol(5-Br-PADAP) in the presence of surfactant Triton X-100. Results The maximum absorption of the complex compound was at 558 nm, the linearity of test was up to 51 μmol/L. The molar absorption coefficient is 1.05×105L/mol-1*cm-1.The recoveries ranged from 98.1%to 103%. The within run and between run CV are 2.1%and 3.6%. Comparing this method (Y) with AAS(X), we obtained the regression equation.Y=1.02X-0.41,r=0.9862,P>0.05. The reference interval (±2s) was (9.5~24.3)μmol/L (n=48). Conclusions This method is simple, sensitive, stable, rapid and accurate for clinical application.
Quantitative detection of HBV-DNA on gel documentation systems
WU Xiaoman;GUO Haibo
2001, 16(03): 144-146.
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Objective To establish polymerase chain reaction-gel documentation systems (PCR-GDS) technology for detecting nucleic acid amplification products. Methods 1.PCR amplification curve was observed with fluorescence quantitative PCR (FQ-PCR), and suitable cycle numbers were selected. 2.Standard HBV-DNA sera (101-106 copies/μl) were amplified 40, 35 and 30 cycles separately. The PCR products were gel electrophoried and EB stained, then analyzed with GDS. Results When cycles were 40 and 35, linearity was good in standard sera 101-104 copies/μl, but line was nearly flat in 104-106 copies/μl; when cycles were 32, 10 copies/μl can not be detected, and linearity was good in 102-106 copies/μl (r=0.98). Conclusion 32 cycles were suitable cycle number for PCR-GDS. Among quantitative analysis of GDS, parameters of volume and volume percentage were better than others.
微生物学检验论著
Screening urinary tract infections by nitroblue tetrazolium reduction test
2001, 16(03): 147-149.
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Evaluation of CHROMagar Candida medium for identi-fication of candida species
YING Chunmei;YANG Haihui;Zhang Huifang
2001, 16(03): 150-152.
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Haemophilus spp. in 204 children with respiratory tract infection: isolation, identification and drug resistance anylysis
2001, 16(03): 151-152.
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临床检验与血液学论著
The detection of p16 gene pure detection in leukemia cells and its clinical significance
2001, 16(03): 158-159.
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Measuring DNA fragments by diphenylamine to analys isleukemic cell apoptosis
2001, 16(03): 160-162.
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生物化学检验论著
A kinetic cplasimetric method for the determination of lipase activity in serum and its clinical application
2001, 16(03): 164-165.
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Protective effect of trehalose on serum enzymes in the process of freeze-drying and rehydration
2001, 16(03): 166-167.
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The Procedure for screening microalbumin in urine easily and rapidiy with bromphenol blue
NI Fangrong;DENG Yibin
2001, 16(03): 168-171.
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免疫学检验论著
Results analysis of fluorescence quantitative PCR application in detection of Neisseria Gonococci Chlamydia Trach- omatis and Ureaplasma Urealyticum
SUN Jimei;Zhang Zhijie;Shao Yang
2001, 16(03): 172-173.
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