Abstract: Objective To develop a simple and rapid method for detecting V. cholerae O1. Methods Staphylococcus aureus Cowan I was coated with anti-V. cholerae O1 monoclonal antibodies and a coagglutination test(COA) detecting V. cholerae O1 in 6-h fecal enrichment cultures was tested. Results The detect limit of COA was 4×106 CFU/ml, and detect time was 5 min. When 92 samples were tested, the diagnostic specificity, sensitivity and accuracy of the test, compared with standard culture methods, were 100%、95.5%、97.8%，respectively. Conclusion The COA is simple and less time-consuming, and can be used as a new method for rapid detection of V. Cholerae.