Abstract: Objective To establish polymerase chain reaction-gel documentation systems (PCR-GDS) technology for detecting nucleic acid amplification products. Methods 1.PCR amplification curve was observed with fluorescence quantitative PCR (FQ-PCR), and suitable cycle numbers were selected. 2.Standard HBV-DNA sera (101-106 copies/μl) were amplified 40, 35 and 30 cycles separately. The PCR products were gel electrophoried and EB stained, then analyzed with GDS. Results When cycles were 40 and 35, linearity was good in standard sera 101-104 copies/μl, but line was nearly flat in 104-106 copies/μl; when cycles were 32, 10 copies/μl can not be detected, and linearity was good in 102-106 copies/μl (r=0.98). Conclusion 32 cycles were suitable cycle number for PCR-GDS. Among quantitative analysis of GDS, parameters of volume and volume percentage were better than others.