Abstract: Objective To establish the genotyping method of the Duffy blood group.Methods According to the sequence of cDNA coding for FY glycoprotein,the Fya/Fyb primers were synthesized,and the genotyping method with PCR-SSP was established, The Duffy blood group of 102 unrelated donors in Zhejiang have been investigated by this method.Results Complete correlation between Fy phenotypes and genotypes was obtained in all samples studied. In all of 102 Zhejiang Han populations, 91 were Fya/Fya homozygotes(89.2%), 10 were Fya/Fyb heterozygotes(8.8%),and 1 was Fyb/Fyb homozygote(0.98%).The gene frequency of Fya was 0.9412,compared with that 0.0588 of Fyb.Conclution The genotyping method of Duffy blood group with PCR-SSP was simple and rapid, and suitable for clinical application. The Fya gene was the major gene of Duffy blood group in Zhejiang Han population.