Regulation of miR-338-3p on proliferation,invasion,migration and epithelial-mesenchymal transformation of thyroid papillary carcinoma cell line TPC-1

doi:10.3969/j.issn.1673-8640.2022.06.016

Abstract

Abstract:

Objective To investigate the effect of microRNA（miR）-338-3p overexpression of sirtuin （SIRT）6 on proliferation,invasion,migration and epithelial-mesenchymal transformation（EMT） of human thyroid papillary carcinoma cell line TPC-1. Methods Luciferase reporting assay was used to detect the targeting relationship between miR-338-3p and SIRT6. SIRT6 pcDNA vector was constructed to overexpress SIRT6,and miR-338-3p mimic and pcDNA-SIRT6 were transfected into TPC-1 cells either alone or in combination. The transfected plasmids were classified into 6 groups,including control group（no treatment）,mimic-NC group（transfected mimic-NC） and miR-338-3p mimic group（transfected miR-338-3p mimic）,pcDNA-SIRT6 group（transfected pcDNA-SIRT6）,mimic-NC+pcDNA-SIRT6 group（co-transfected mimic-NC and pcDNA-SIRT6） and miR-338-3p mimic+pcDNA-SIRT6 group（co-transfected with miR-338-3p mimic and pcDNA-SIRT6）. The clone genesis rate,invasive cell number,scratch healing rate of TPC-1 cells and the expression levels of E-cadherin（E-cad）,N-cadherin（N-cad） and Vimentin in epithelial cells were determined. Results The target gene of miR-338-3p was SIRT6. Compared with the control group,clone genesis rate,invasive cell number and scratch healing rate in miR-338-3p mimic group were decreased（P<0.05）. The protein expression of E-cad was increased（P<0.05）,while the protein expressions of N-cad and Vimentin were decreased（P<0.05）. The clone genesis rate,invasive cell number and scratch healing rate were increased in pcDNA+SIRT6 group. The protein expression level of E-cad was decreased（P<0.05）,while the protein expressions of N-cad and Vimentin were increased（P<0.05）. Compared with pcDNA-SIRT6 group,clone genesis rate,invasive cell number and scratch healing rate in miR-338-3p mimic+pcDNA-SIRT6 group were decreased（P<0.05）,while E-cad protein expression was increased（P<0.05）. The protein expressions of N-cad and Vimentin were decreased（P<0.05）. Conclusions The miR-338-3p overexpression targets SIRT6 to inhibit TPC-1 cell proliferation,invasion,migration and EMT.

Key words: MicroRNA-338-3p, Sirtuin 6, Thyroid papillary carcinoma

CLC Number:

R446.7

LI Tao, LI Jia, PAN Jing, LIANG Xiao, YU Lina. Regulation of miR-338-3p on proliferation,invasion,migration and epithelial-mesenchymal transformation of thyroid papillary carcinoma cell line TPC-1[J]. Laboratory Medicine, 2022, 37(6): 583-589.

Figures/Tables 7

References 18

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