Abstract:

Objective To investigate the clinical roles of relative expression levels of serum miR-150-5p and latent transforming growth factor-beta binding protein（LTBP）-2 in evaluating the condition of acute cerebral infarction（ACI）. Methods Totally，121 ACI patients（ACI group）from Yiwu Fuyuan Private Hospital from May 2018 to February 2021 were enrolled and classified into small infarction group，medium infarction group and large infarction group based on the volume of cerebral infarction within 48-72 h of onset. According to the National Institute of Health Stroke Scale（NIHSS） score，ACI patients were classified into mild group，moderate group and severe group. A total of 128 healthy subjects were enrolled as healthy control group. The relative expression levels of miR-150-5p and LTBP-2 mRNA in serum were determined. Pearson correlation analysis was used to evaluate the correlation between miR-150-5p and LTBP-2 mRNA in ACI patients. Logistic regression analysis was used to evaluate the risk factors of ACI occurrence. Results Compared with healthy control group，the relative expression level of miR-150-5p in serum of ACI group was decreased（P<0.001），while the relative expression level of LTBP-2 mRNA was increased（P<0.001）. The relative expression levels of miR-150-5p in serum of small infarction group，medium infarction group and large infarction group were decreased in turn（P<0.001），while the relative expression levels of LTBP-2 mRNA were increased（P<0.001）. The relative expression levels of miR-150-5p in serum of mild，moderate and severe groups were decreased in turn（P<0.001），while the relative expression levels of LTBP-2 mRNA were increased（P<0.001）. The prediction results of Targetscan website showed that LTBP-2 was a potential target gene for miR-150-5p. Pearson correlation analysis showed a negative correlation between serum miR-150-5p and LTBP-2 mRNA expression levels in ACI group（r=-0.346，P<0.001）. The results of multivariate Logistic regression analysis showed that decreased expression level of miR-150-5p and increased expression level of LTBP-2 mRNA were risk factors for the occurrence of ACI [odds ratios（OR） were 0.563 and 1.698，95% confidence intervals（CI）were 0.445-0.712 and 1.342-2.148，respectively]. Conclusions The relative expression levels of miR-150-5p and LTBP-2 in serum of ACI patients are related to disease severity，and they can be used as the indicators for evaluating the condition of ACI.

Key words: MicroRNA-150-5p, Latent transforming growth factor-beta binding protein-2, Acute cerebral infarction