Abstract:

Objective To investigate the relation between serum miR-140-5p and peroxisome-proliferator-activated receptor gamma coactivator-1 alpha（PGC-1α） mRNA expression and vascular cognitive impairment（VCI） in acute ischemic stroke（AIS）patients. Methods Totally，163 patients with AIS in Shijiazhuang People's Hospital from January 2020 to January 2022（AIS group） were enrolled and classified into VCI group and non-VCI group according to whether they had VCI after 3 months or not. Totally，57 healthy subjects were enrolled as control group. The general data of all the subjects were collected，and serum miR-140-5p and PGC-1α mRNA expression were determined. Logistic regression analysis was used to analyze the factors on VCI in AIS patients，and receiver operating characteristic（ROC） curve was used to analyze the predictive value of serum miR-140-5p and PGC-1α mRNA expression on VCI in AIS patients. Results Serum miR-140-5p expression in AIS group was higher than that in control group（P<0.001），and PGC-1α mRNA expression was lower than that in control group（P<0.001）. The miR-140-5p expression was higher in VCI group than that in non-VCI group（P<0.05），and PGC-1α mRNA expression was lower than that in non-VCI group（P<0.05）. Increased the National Institute of Health Stroke Scale（NIHSS） score [odds ratio（OR）=3.298，95% confidence interval（CI）1.983-5.485]，increased miR-140-5p expression（OR=1.438，95%CI 1.147-1.801） and decreased PGC-1α mRNA expression（OR=0.716，95%CI 0.585-0.876） were risk factors for VCI in AIS patients. The areas under curves（AUC） of miR-140-5p and PGC-1α mRNA expression single and combined determinations for VCI in AIS patients were 0.790，0.780 and 0.905，respectively. Conclusions Increased serum miR-140-5p expression and decreased PGC-1α mRNA expression are related with VCI in AIS patients and may be predictors of VCI in AIS patients.

Key words: MicroRNA-140-5p, Peroxisome-proliferator-activated receptor gamma coactivator-1 alpha, Acute ischemic stroke, Vascular cognitive impairment