Role of berberine on anti-platelet aggregation in children with Kawasaki disease in vitro

doi:10.3969/j.issn.1673-8640.2020.11.017

Abstract

Abstract:

Objective To investigate the feasibility of berberine（BB） in the treatment of Kawasaki disease（KD） in children,and to provide a reference for KD treatment. Methods A total of 71 ethylene diamine tetraacetic acid anticoagulant blood samples were collected from healthy children and children with KD,respectively. Platelet（PLT） rich plasma（PRP） was separated,which was washed with 0.9% NaCl and added with 5 mg/mL BB. Adenosine diphosphate was used to induce aggregation in vitro. The aggregation rate was determined. Results After BB was added,the PLT aggregation rate decreased with statistical significance（P<0.05）. Conclusions BB has a good ability of anti-PLT aggregation in vitro. It can be considered to further investigate the application of BB in anti-thrombosis treatment of KD patients.

Key words: Berberine, Kawasaki disease, Platelet aggregation, Children

CLC Number:

R446.1

LIU Yunfeng, ZHENG Hao, CHE Di, PI Lei, FU Lanyan, GU Xiaoqiong. Role of berberine on anti-platelet aggregation in children with Kawasaki disease in vitro[J]. Laboratory Medicine, 2020, 35(11): 1158-1160.

Figures/Tables 2

References 11

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时间/min	90×g	110×g	130×g
5	181.6 （167.0~224.4）	228.5 （192.8~253.0）	211.5 （197.0~224.1）
8	201.7 （187.0~209.3）	211.3 （200.1~223.3）	207.2 （193.0~211.2）
10	215.3 （197.0~227.5）	195.1 （179.8~215.3）	193.7 （178.0~203.4）
15	204.1 （191.0~213.4）	190.3 （178.7~205.7）	185.1 （169.0~204.5）

时间/min	90×g	110×g	130×g
5	181.6 （167.0~224.4）	228.5 （192.8~253.0）	211.5 （197.0~224.1）
8	201.7 （187.0~209.3）	211.3 （200.1~223.3）	207.2 （193.0~211.2）
10	215.3 （197.0~227.5）	195.1 （179.8~215.3）	193.7 （178.0~203.4）
15	204.1 （191.0~213.4）	190.3 （178.7~205.7）	185.1 （169.0~204.5）

组别	例数	加BB前	加BB后	t值	P值
对照组	71	26.25±3.32	10.32±2.11^*	9.523	<0.05
KD组	71	18.32±2.51	6.23±1.59^*	10.987	<0.05

组别	例数	加BB前	加BB后	t值	P值
对照组	71	26.25±3.32	10.32±2.11^*	9.523	<0.05
KD组	71	18.32±2.51	6.23±1.59^*	10.987	<0.05

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