Abstract:

Objective To determine the expressions of CD55,CD59 and fluorescent aerolysin（FLAER） of anemia patients,and to evaluate the role of CD55,CD59 and FLAER combined determination for the diagnosis of paroxysmal nocturnal hemoglobinuria（PNH）. Methods A total of 157 inpatients,including 38 patients with iron deficiency anemia（IDA）,23 patients with megaloblastic anemia（MA）,27 patients with myelodysplastic syndrome（MDS）,25 patients with aplastic anemia（AA）,31 patients with autoimmune hemolytic anemia（AIAH） and 13 patients with PNH,were enrolled. Totally,20 healthy subjects were enrolled as control group. Erythrocyte and granulocyte CD55,CD59 and granulocyte and monocyte FLAER were determined. Results Erythrocyte and granulocyte CD55^- and CD59^- percentages in PNH group had statistical significance compared with control group（P<0.05）. In PNH group,granulocyte and monocyte FLAER^- percentages were >50%,which were higher than erythrocyte and granulocyte CD55^- and CD59^- percentages（P<0.05）. The consistency rate of CD55,CD59 and FLAER determinations was 100%. There were 3 AA patients and 2 MDS patients with FLAER deletion and normal CD55 and CD59 expressions. There were 2 AIHA patients with partial erythrocyte CD55 and CD59 deletion and normal granulocyte CD55 and CD59 and FLAER expression. Conclusions CD55 and CD59 expression defects are the main characteristics of PNH,and FLAER determination combined with CD55 and CD59 plays a role in the diagnosis and differential diagnosis of PNH.

Key words: CD55, CD59, Fluorescent aerolysin, Anemia, Paroxysmal nocturnal hemoglobinuria