Changes of neutrophil oxidative phagocytosis and related receptors in sputum samples of COPD patients

doi:10.3969/j.issn.1673-8640.2020.06.012

Abstract

Abstract:

Objective To investigate the roles of neutrophil oxidative phagocytosis and related receptors [chemokine C-C receptor -1（CCR1）,Toll-like receptor（TLR） 2 and TLR4] in patients with chronic obstructive pulmonary disease（COPD）. Methods A flow cytometry method was established to determine neutrophil oxidative phagocytosis and related receptors in sputum samples of COPD patients. By flow cytometry,neutrophil oxidative phagocytosis in peripheral blood and sputum samples of 30 COPD patients（COPD group）,30 pneumonia patients（pneumonia group） and 30 healthy subjects（healthy control group） was determined. Neutrophil oxidative phagocytosis and the expression levels of CCR1,TLR2 and TLR4 on the surface were determined. Results The neutrophil oxidative phagocytosis of peripheral blood in pneumonia group and COPD group was lower than that in healthy control group（P<0.05）,and there was no statistical significance between pneumonia and COPD groups（P>0.05）. There was statistical significance in the expression of CCR1 on the surface of peripheral blood neutrophils among COPD,pneumonia and healthy control groups（P<0.05）,but there was no statistical significance for TLR2 and TLR4 expression levels（P>0.05）. The neutrophil oxidative phagocytosis in sputum samples in COPD group was lower than that in pneumonia group（P<0.05）,the expression level of CCR1 was higher than that in pneumonia group（P<0.05）,and there was no statistical significance for the expression levels of TLR2 and TLR4（P>0.05）. Pearson correlation analysis showed that the positive rate of neutrophil oxidative phagocytosis in sputum samples in COPD group was negatively correlated with the expression level of CCR1（r=-0.548,P<0.01）,and it had no correlation with the expression levels of TLR2 and TLR4（r=-0.206 and -0.219,P>0.05）. Conclusions Neutrophil oxidative phagocytosis in sputum samples of COPD patients is decreased,which can increase CCR1 expression level on the surface,suggesting that COPD patients'respiratory neutrophil oxidative phagocytosis injury may be one of the mechanisms of COPD pathogenesis.

Key words: Chemokine C-C receptor-1, Toll-like receptor, Neutrophil, Oxidative phagocytosis, Chronic obstructive pulmonary disease

CLC Number:

R446.1

YAN Peiyi, ZHANG Ji, TU Huanping, ZHANG Li, QIU Yue, ZHANG Fengying, HU Yiwen, JIN Shu. Changes of neutrophil oxidative phagocytosis and related receptors in sputum samples of COPD patients[J]. Laboratory Medicine, 2020, 35(6): 570-577.

Figures/Tables 8

References 21

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组别	例数	氧化吞噬百分率	TLR2	TLR4	CCR1
正常对照组	30	98.27± 2.58	59.62±19.32	48.70±27.57	61.77±25.89
肺炎组	30	93.05±9.24^*	54.33±32.86	40.62±22.93	29.04±28.16^*
COPD组	30	96.22± 3.28^*	45.58±34.31	42.45±28.62	40.97±34.58^#

组别	例数	氧化吞噬百分率	TLR2	TLR4	CCR1
正常对照组	30	98.27± 2.58	59.62±19.32	48.70±27.57	61.77±25.89
肺炎组	30	93.05±9.24^*	54.33±32.86	40.62±22.93	29.04±28.16^*
COPD组	30	96.22± 3.28^*	45.58±34.31	42.45±28.62	40.97±34.58^#

组别	例数	氧化吞噬百分率	TLR2	TLR4	CCR1
肺炎组	30	51.57±36.50	10.26±9.24	20.86±18.39	15.08±15.03
COPD组	30	33.60±31.73^*	12.64±12.13	20.30±19.15	44.99±34.11^*

组别	例数	氧化吞噬百分率	TLR2	TLR4	CCR1
肺炎组	30	51.57±36.50	10.26±9.24	20.86±18.39	15.08±15.03
COPD组	30	33.60±31.73^*	12.64±12.13	20.30±19.15	44.99±34.11^*