Abstract:

Objective To investigate the role of severe acute respiratory syndrome coronavirus 2（SARS-CoV-2） IgM and IgG antibody detections in the diagnosis of corona virus disease 2019（COVID-19）. Methods A total of 173 patients diagnosed with COVID-19 were enrolled. There were 140 cases of being positive for the first time of SARS-CoV-2 nucleic acid test and 33 cases of being eventually diagnosed for several times of SARS-CoV-2 nucleic acid test. Totally,101 patients without COVID-19 were enrolled as control group. Colloidal gold immunochromatography assay（CGIA） was used to detect SARS-CoV-2 IgM and IgG antibodies,and fluorescence quantitative polymerase chain reaction（PCR） was used to detect SARS-CoV-2 nucleic acid. Taking clinical diagnosis as gold standard,the efficiency of SARS-CoV-2 IgM and IgG antibody detections in the diagnosis of COVID-19 was analyzed. Results The sensitivities of SARS-CoV-2 IgM and IgG antibody detections were 76.9% and 74.6%,the specificities were 94.0% and 95.1%,and the consistency rates were 83.2% and 82.1%,which were well consistent with the clinical diagnosis（kappa values were 0.663 and 0.644）,respectively. Receiver operating characteristic（ROC） curve analysis showed that the areas under curves（AUC） of SARS-CoV-2 IgM and IgG antibody detections for the diagnosis of COVID-19 were 0.85. Conclusions The detections of SARS-CoV-2 antibodies have high sensitivities,specificities and consistency rates,which can be used for the auxiliary diagnosis of COVID-19.

Key words: Severe acute respiratory syndrome coronavirus 2, Antibody, Corona virus disease 2019