Role of Xpert MTB/RIF Ultra in the detection of Mycobacterium tuberculosis and rifampicin resistance

doi:10.3969/j.issn.1673-8640.2019.08.0015

Abstract

Abstract:

Objective To evaluate the role of Xpert MTB/RIF Ultra in the detection of Mycobacterium tuberculosis and rifampicin resistance by comparing with Xpert MTB/RIF. Methods Patients with pulmonary tuberculosis symptoms were enrolled and classified into case detection group（CDG） and multidrug-resistance risk group（DRG）. The sputum samples of the 2 groups were detected by Xpert MTB/RIF and Xpert MTB/RIF Ultra,respectively. The MGIT960 liquid culturing was used as a reference method to compare the sensitivities and specificities in the detection of Mycobacterium tuberculosis and rifampicin resistance. Results The sensitivity of Xpert MTB/RIF Ultra in the detection of Mycobacterium tuberculosis was 96.1%,which was higher than that of Xpert MTB/RIF（85.2%）. The specificities were the same. Xpert MTB/RIF Ultra was sensitive in the detection of tuberculosis with negative acid-fast staining smear,and the sensitivity（75.5%） was higher than that of Xpert MTB/RIF（22.6%）. Conclusions Xpert MTB/RIF Ultra is superior to Xpert MTB/RIF in the detection of tuberculosis,especially in patients with negative acid-fast staining smear tuberculosis.

Key words: Xpert MTB/RIF Ultra, Xpert MTB/RIF, Mycobacterium tuberculosis, Rifampicin, Drug resistance

CLC Number:

R446.1

WANG Wei, LI Jing, LI Zheng, YANG Jing, YUAN Xing, ZHANG Guolong, CHEN Huihui. Role of Xpert MTB/RIF Ultra in the detection of Mycobacterium tuberculosis and rifampicin resistance[J]. Laboratory Medicine, 2019, 34(8): 742-745.

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