Abstract:

Objective To know the status of serum cholinesterase（CHE） determination in Shanghai through investigation,and to provide a reference for the external quality assessment of CHE determination in Shanghai.Methods Quality control samples with 4 different levels basing on human serum matrix were delivered to 69 laboratories with different grades and types in Shanghai. All samples were determined within prescribed time,and determination results were reported in unified time. The results of laboratories were classified according to determination methods and reagent brands. Results The 69 laboratories used 3 determination methods,and were classified into 4 determination method groups,German Society for Clinical Chemistry（Deutsche Gesellschaft für Klinische Chemie,DGKC） colorimetric method with butyrylthiocholine/potassium hexacyanochromate（Ⅲ） as substrate,colorimetric method with butyrylthiocholine /5,5'-dithiobis（2-nitrobenzoic acid）（DTNB） as substrate,enzyme coupling method recommended by Japan Society of Clinical Chemistry（JSCC） and dry chemical DGKC method. The intra-group variation of the 4 groups was good [coefficients of variation（CV）<10%]. The results of JSCC method group were different from those of the other 3 groups,and the results of DGKC method and dry chemical DGKC method group had difference,but the results' difference between DGKC method and DTNB method groups was small,and the bias was <3%. CHE determination in Shanghai mainly（about 85%） was based on DGKC method and DTNB method as reaction principle,the numbers of reagent brands of the 2 methods was equal,however,the number of laboratories using DGKC method reagents（38） was more than the number of laboratories using DTNB method reagents（14）.Conclusions The results of this investigation provide a reference for the external quality assessment of CHE determination in Shanghai.

Key words: Cholinesterase, Determination method, External quality assessment